CHIT_ACTCC
ID CHIT_ACTCC Reviewed; 322 AA.
AC P86473; A0A2R6RNH9;
DT 20-APR-2010, integrated into UniProtKB/Swiss-Prot.
DT 13-NOV-2019, sequence version 2.
DT 25-MAY-2022, entry version 20.
DE RecName: Full=Endochitinase {ECO:0000250|UniProtKB:P06215};
DE EC=3.2.1.14;
DE Flags: Precursor;
GN ORFNames=CEY00_Acc04863 {ECO:0000312|EMBL:PSS31588.1};
OS Actinidia chinensis var. chinensis (Chinese soft-hair kiwi).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; Ericales; Actinidiaceae; Actinidia.
OX NCBI_TaxID=1590841;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Red5;
RX PubMed=29661190; DOI=10.1186/s12864-018-4656-3;
RA Pilkington S.M., Crowhurst R., Hilario E., Nardozza S., Fraser L., Peng Y.,
RA Gunaseelan K., Simpson R., Tahir J., Deroles S.C., Templeton K., Luo Z.,
RA Davy M., Cheng C., McNeilage M., Scaglione D., Liu Y., Zhang Q., Datson P.,
RA De Silva N., Gardiner S.E., Bassett H., Chagne D., McCallum J.,
RA Dzierzon H., Deng C., Wang Y.Y., Barron L., Manako K., Bowen J.,
RA Foster T.M., Erridge Z.A., Tiffin H., Waite C.N., Davies K.M.,
RA Grierson E.P., Laing W.A., Kirk R., Chen X., Wood M., Montefiori M.,
RA Brummell D.A., Schwinn K.E., Catanach A., Fullerton C., Li D.,
RA Meiyalaghan S., Nieuwenhuizen N., Read N., Prakash R., Hunter D., Zhang H.,
RA McKenzie M., Knabel M., Harris A., Allan A.C., Gleave A., Chen A.,
RA Janssen B.J., Plunkett B., Ampomah-Dwamena C., Voogd C., Leif D.,
RA Lafferty D., Souleyre E.J.F., Varkonyi-Gasic E., Gambi F., Hanley J.,
RA Yao J.L., Cheung J., David K.M., Warren B., Marsh K., Snowden K.C.,
RA Lin-Wang K., Brian L., Martinez-Sanchez M., Wang M., Ileperuma N.,
RA Macnee N., Campin R., McAtee P., Drummond R.S.M., Espley R.V.,
RA Ireland H.S., Wu R., Atkinson R.G., Karunairetnam S., Bulley S.,
RA Chunkath S., Hanley Z., Storey R., Thrimawithana A.H., Thomson S.,
RA David C., Testolin R., Huang H., Hellens R.P., Schaffer R.J.;
RT "A manually annotated Actinidia chinensis var. chinensis (kiwifruit) genome
RT highlights the challenges associated with draft genomes and gene prediction
RT in plants.";
RL BMC Genomics 19:257-257(2018).
RN [2] {ECO:0000305}
RP PROTEIN SEQUENCE OF 22-29, AND ALLERGEN.
RC STRAIN=cv. Hort 16A {ECO:0000269|PubMed:15536427};
RC TISSUE=Fruit {ECO:0000269|PubMed:15536427};
RX PubMed=15536427; DOI=10.1016/j.jaci.2004.07.016;
RA Bublin M., Mari A., Ebner C., Knulst A., Scheiner O.,
RA Hoffmann-Sommergruber K., Breiteneder H., Radauer C.;
RT "IgE sensitization profiles toward green and gold kiwifruits differ among
RT patients allergic to kiwifruit from 3 European countries.";
RL J. Allergy Clin. Immunol. 114:1169-1175(2004).
CC -!- FUNCTION: Defense against chitin-containing fungal pathogens.
CC {ECO:0000250|UniProtKB:P06215}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide
CC (1->4)-beta-linkages in chitin and chitodextrins.; EC=3.2.1.14;
CC Evidence={ECO:0000250|UniProtKB:P06215};
CC -!- ALLERGEN: Causes an allergic reaction in human. Binds IgE.
CC {ECO:0000269|PubMed:15536427}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 19 family. Chitinase
CC class I subfamily. {ECO:0000255}.
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DR EMBL; NKQK01000004; PSS31588.1; -; Genomic_DNA.
DR AlphaFoldDB; P86473; -.
DR SMR; P86473; -.
DR STRING; 1590841.P86473; -.
DR Allergome; 2408; Act c Chitinase_I.
DR EnsemblPlants; PSS31588; PSS31588; CEY00_Acc04863.
DR Gramene; PSS31588; PSS31588; CEY00_Acc04863.
DR OMA; CEPSATW; -.
DR Proteomes; UP000241394; Chromosome lg4.
DR GO; GO:0008061; F:chitin binding; IEA:UniProtKB-KW.
DR GO; GO:0004568; F:chitinase activity; IEA:UniProtKB-EC.
DR GO; GO:0016998; P:cell wall macromolecule catabolic process; IEA:InterPro.
DR GO; GO:0006032; P:chitin catabolic process; IEA:UniProtKB-KW.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0000272; P:polysaccharide catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 3.30.60.10; -; 1.
DR InterPro; IPR001002; Chitin-bd_1.
DR InterPro; IPR036861; Endochitinase-like_sf.
DR InterPro; IPR016283; Glyco_hydro_19.
DR InterPro; IPR000726; Glyco_hydro_19_cat.
DR InterPro; IPR023346; Lysozyme-like_dom_sf.
DR Pfam; PF00187; Chitin_bind_1; 1.
DR Pfam; PF00182; Glyco_hydro_19; 1.
DR PIRSF; PIRSF001060; Endochitinase; 1.
DR PRINTS; PR00451; CHITINBINDNG.
DR SMART; SM00270; ChtBD1; 1.
DR SUPFAM; SSF53955; SSF53955; 1.
DR SUPFAM; SSF57016; SSF57016; 1.
DR PROSITE; PS50941; CHIT_BIND_I_2; 1.
DR PROSITE; PS00773; CHITINASE_19_1; 1.
DR PROSITE; PS00774; CHITINASE_19_2; 1.
PE 1: Evidence at protein level;
KW Allergen; Carbohydrate metabolism; Chitin degradation; Chitin-binding;
KW Direct protein sequencing; Disulfide bond; Glycosidase; Hydrolase;
KW Plant defense; Polysaccharide degradation; Reference proteome; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000269|PubMed:15536427"
FT CHAIN 22..322
FT /note="Endochitinase"
FT /id="PRO_0000393867"
FT DOMAIN 22..62
FT /note="Chitin-binding type-1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00261"
FT DISULFID 24..39
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00261"
FT DISULFID 33..45
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00261"
FT DISULFID 38..52
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00261"
FT DISULFID 56..60
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00261"
SQ SEQUENCE 322 AA; 34459 MW; DDB619209291C285 CRC64;
MKMRYCVLVS VLAILVIRGS AENCGRQAGG ALCPGGQCCS KWGWCGTTPD HCGTDCQSQC
GGTPSPGPSG SDITNLIPRS TFDRMLLHRN DGACPGKNFY TYDGFIAAAR SFGAFATTGD
TDTRKREIAA FLAQTSHETT GGWASAPDGP YSWGYCFLRE QGNPGAYCTP SAQWPCAPGR
KYFGRGPIQI THNYNYGPAG KALGVDLLNN PDLVATDPAI SFKTALWFWM TPQSPKPSCH
DVITGRWTPS AADRSAGRLP GFGVITNIIN GGLECGHGSD SRVQDRIGFY KRYCQIFGIG
TGDNLDCGNQ RSFGSGRLVD TM
