ID   CHIT_STRVO              Reviewed;          10 AA.
AC   P86977;
DT   01-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2014, sequence version 1.
DT   25-MAY-2022, entry version 12.
DE   RecName: Full=Chitinase {ECO:0000303|PubMed:22915152};
DE            EC=3.2.1.14 {ECO:0000269|PubMed:22915152};
DE   Flags: Fragment;
OS   Streptomyces violaceusniger.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces; Streptomyces violaceusniger group.
OX   NCBI_TaxID=68280;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, ACTIVITY REGULATION,
RP   BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY.
RC   STRAIN=MTCC 3959 {ECO:0000269|PubMed:22915152};
RX   PubMed=22915152; DOI=10.1002/jobm.201100648;
RA   Nagpure A., Gupta R.K.;
RT   "Purification and characterization of an extracellular chitinase from
RT   antagonistic Streptomyces violaceusniger.";
RL   J. Basic Microbiol. 53:429-439(2013).
CC   -!- FUNCTION: Has antifungal activity. Inhibits mycelial growth of wood
CC       rotting fungi P.chrysosporium MTCC 787, S.commune ITCC 3751, G.trabeum
CC       MTCC 355, P.agaricans ITCC 761, C.versicolor MTCC 138, P.versicolor
CC       ITCC 13, P.placenta MTCC 144 and P.friabilis ITCC 335.
CC       {ECO:0000269|PubMed:22915152}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide
CC         (1->4)-beta-linkages in chitin and chitodextrins.; EC=3.2.1.14;
CC         Evidence={ECO:0000269|PubMed:22915152};
CC   -!- ACTIVITY REGULATION: Inhibited by Hg(2+) and, to a lesser extent, by
CC       Ag(+). Hardly or not at all affected by Fe(3+), Mg(2+), Co(2+), Zn(2+),
CC       Ca(2+), K(+), EDTA, beta-mercaptoethanol and N-acetylglucosamine.
CC       {ECO:0000269|PubMed:22915152}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=0.143 mM for p-nitrophenyl-N-acetyl-beta-D-N,N'-diacetylchitobiose
CC         {ECO:0000269|PubMed:22915152};
CC         Vmax=6.6 umol/h/mg enzyme with p-nitrophenyl-N-acetyl-beta-D-N,N'-
CC         diacetylchitobiose as substrate {ECO:0000269|PubMed:22915152};
CC       pH dependence:
CC         Optimum pH is 5 at 37 degrees Celsius. Retains >80% activity after
CC         incubation at pH 3-10 for 1 hour. {ECO:0000269|PubMed:22915152};
CC       Temperature dependence:
CC         Optimum temperature is 50 degrees Celsius. Retains only 60% and 25%
CC         activity after incubation at 30 degrees Celsius and 60 degrees
CC         Celsius for 1 hour, respectively. {ECO:0000269|PubMed:22915152};
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:22915152}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004568; F:chitinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006032; P:chitin catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR   GO; GO:0000272; P:polysaccharide catabolic process; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Antimicrobial; Carbohydrate metabolism; Chitin degradation;
KW   Direct protein sequencing; Fungicide; Glycosidase; Hydrolase;
KW   Polysaccharide degradation; Secreted.
FT   CHAIN           1..>10
FT                   /note="Chitinase"
FT                   /id="PRO_0000430498"
FT   NON_TER         10
FT                   /evidence="ECO:0000303|PubMed:22915152"
SQ   SEQUENCE   10 AA;  811 MW;  386BFC68676861A8 CRC64;
     GDGTGPGPGP