CHIT_ZIZMA
ID CHIT_ZIZMA Reviewed; 330 AA.
AC Q2VST0;
DT 26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT 10-JAN-2006, sequence version 1.
DT 25-MAY-2022, entry version 52.
DE RecName: Full=Endochitinase Ziz m 1.0101 {ECO:0000305};
DE EC=3.2.1.14 {ECO:0000269|PubMed:16122801};
DE AltName: Full=Allergen Ziz m 1 {ECO:0000303|PubMed:16122801, ECO:0000303|PubMed:18435802, ECO:0000312|EMBL:AAX40948.1};
DE AltName: Allergen=Ziz m 1.0101 {ECO:0000305};
DE Flags: Precursor;
OS Ziziphus mauritiana (Indian jujube).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Rosales; Rhamnaceae; Paliureae; Ziziphus.
OX NCBI_TaxID=157914;
RN [1] {ECO:0000312|EMBL:AAX40948.1}
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 146-154, CATALYTIC
RP ACTIVITY, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY, AND
RP ALLERGEN.
RC TISSUE=Flower bud {ECO:0000303|PubMed:16122801};
RX PubMed=16122801; DOI=10.1016/j.molimm.2005.07.021;
RA Lee M.F., Hwang G.Y., Chen Y.H., Lin H.C., Wu C.H.;
RT "Molecular cloning of Indian jujube (Zizyphus mauritiana) allergen Ziz m 1
RT with sequence similarity to plant class III chitinases.";
RL Mol. Immunol. 43:1144-1151(2006).
RN [2]
RP ALLERGEN, AND REGIONS.
RX PubMed=18435802; DOI=10.1111/j.1365-2249.2008.03661.x;
RA Lee M.F., Tsai J.J., Hwang G.Y., Lin S.J., Chen Y.H.;
RT "Identification of immunoglobulin E (IgE)-binding epitopes and recombinant
RT IgE reactivities of a latex cross-reacting Indian jujube Ziz m 1
RT allergen.";
RL Clin. Exp. Immunol. 152:464-471(2008).
RN [3]
RP ALLERGEN.
RX PubMed=22289730; DOI=10.1016/j.anai.2011.10.011;
RA Lee M.F., Lin S.J., Wang N.M., Wu H.J., Chen Y.H.;
RT "Plant chitinase III Ziz m 1 stimulates multiple cytokines, most
RT predominantly interleukin-13, from peripheral blood mononuclear cells of
RT latex-fruit allergic patients.";
RL Ann. Allergy Asthma Immunol. 108:113-116(2012).
CC -!- FUNCTION: Defense against chitin containing fungal pathogens
CC (Probable). {ECO:0000305}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide
CC (1->4)-beta-linkages in chitin and chitodextrins.; EC=3.2.1.14;
CC Evidence={ECO:0000269|PubMed:16122801};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:16122801}.
CC -!- ALLERGEN: Causes an allergic reaction in human. Binds to IgE of
CC patients allergic to both the Indian jujube and latex (PubMed:16122801,
CC PubMed:18435802, PubMed:22289730). Natural protein binds to IgE of
CC patients allergic to the fruit of the Indian jujube. Recombinant
CC protein binds to IgE in 87.5% of the 8 latex- and Indian jujube-
CC allergic patients tested (PubMed:16122801). May stimulate allergic
CC inflammation by inducing T-helper type 2 (Th2)-specific immune
CC responses including increased production of cytokines, most
CC predominantly IL13, from peripheral blood mononuclear cells (PBMC) of
CC patients allergic to both latex and the fruit of the Indian jujube.
CC Chitinase activity is required for this function (PubMed:22289730).
CC {ECO:0000269|PubMed:16122801, ECO:0000269|PubMed:18435802,
CC ECO:0000269|PubMed:22289730}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 18 family. Chitinase
CC class II subfamily. {ECO:0000305}.
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DR EMBL; AY839230; AAX40948.1; -; mRNA.
DR AlphaFoldDB; Q2VST0; -.
DR SMR; Q2VST0; -.
DR Allergome; 2487; Ziz m 1.
DR Allergome; 3537; Ziz m 1.0101.
DR CAZy; GH18; Glycoside Hydrolase Family 18.
DR GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR GO; GO:0008843; F:endochitinase activity; IDA:UniProtKB.
DR GO; GO:0006040; P:amino sugar metabolic process; IDA:UniProtKB.
DR GO; GO:0006032; P:chitin catabolic process; IDA:UniProtKB.
DR GO; GO:0050832; P:defense response to fungus; IC:UniProtKB.
DR GO; GO:0000272; P:polysaccharide catabolic process; IEA:UniProtKB-KW.
DR CDD; cd02877; GH18_hevamine_XipI_class_III; 1.
DR InterPro; IPR045321; Cts1-like.
DR InterPro; IPR001223; Glyco_hydro18_cat.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR Pfam; PF00704; Glyco_hydro_18; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
DR PROSITE; PS51910; GH18_2; 1.
PE 1: Evidence at protein level;
KW Allergen; Carbohydrate metabolism; Chitin degradation;
KW Direct protein sequencing; Disulfide bond; Glycosidase; Hydrolase;
KW Plant defense; Polysaccharide degradation; Secreted; Signal.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..330
FT /note="Endochitinase Ziz m 1.0101"
FT /evidence="ECO:0000255"
FT /id="PRO_5004217981"
FT DOMAIN 26..330
FT /note="GH18"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01258"
FT REGION 72..86
FT /note="Binds to IgE in 70% of the 10 patients tested
FT allergic to Indian jujube and latex"
FT /evidence="ECO:0000269|PubMed:18435802"
FT REGION 292..301
FT /note="Binds to IgE in 100% of the 10 patients tested
FT allergic to Indian jujube and latex; sufficient for
FT prediction of the presence of allergic reactions in these
FT patients"
FT /evidence="ECO:0000269|PubMed:18435802"
FT REGION 300..311
FT /note="Binds to IgE in 70% of the 10 patients tested
FT allergic to Indian jujube and latex"
FT /evidence="ECO:0000269|PubMed:18435802"
FT REGION 309..320
FT /note="Binds to IgE in 70% of the 10 patients tested
FT allergic to Indian jujube and latex"
FT /evidence="ECO:0000269|PubMed:18435802"
FT DISULFID 47..90
FT /evidence="ECO:0000250|UniProtKB:P23472"
FT DISULFID 77..80
FT /evidence="ECO:0000250|UniProtKB:P23472"
FT DISULFID 187..219
FT /evidence="ECO:0000250|UniProtKB:P23472"
SQ SEQUENCE 330 AA; 36424 MW; C920E91F25A98C19 CRC64;
MVPQAKLVVA SLILTSALIQ TSEAVGGIAT YWGQYTETEE GSLAEACASN LYSYINIAYL
NIFGEGRYLS LNISGHCSDC TFLGEEIKAC QSQGVKIFLS LGGPYGDYHL TTDGDADRVA
EQLWSSFLGG SKSTGVYQPL LGDVELDGID LDIQIGPPEE YDVLARNLKD LTKDRTRPFY
LSAAPKCSAY NDSDAYLWTA VETGLFDFVW VKFYNDTSCQ YNNDTAAGLD AFYRSWYDWT
VSLAEGNKLL IGIPASNETD NSPLGGYIPS DVLNDQIVSV IMTSSKFGGV NVWNRYYDLK
TNYSSSIILE YVNSGTKYLP LRTKFMYQNA
