CHL1_ARATH
ID CHL1_ARATH Reviewed; 459 AA.
AC Q9LUJ8; Q4V3A4;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Disease resistance protein CHL1 {ECO:0000303|PubMed:23617639};
DE EC=3.2.2.6 {ECO:0000255|PROSITE-ProRule:PRU00204};
DE AltName: Full=Protein CHILLING SENSITIVE 1-LIKE 1 {ECO:0000303|PubMed:23617639};
DE Short=CHS1-LIKE 1 {ECO:0000303|PubMed:23617639};
GN Name=CHL1 {ECO:0000303|PubMed:23617639};
GN OrderedLocusNames=At5g40090 {ECO:0000312|Araport:AT5G40090};
GN ORFNames=MUD12.6 {ECO:0000312|EMBL:BAA97356.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 50-459.
RC STRAIN=cv. Columbia;
RA Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF Clones.";
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY.
RX PubMed=12366802; DOI=10.1046/j.1365-313x.2002.01404.x;
RA Meyers B.C., Morgante M., Michelmore R.W.;
RT "TIR-X and TIR-NBS proteins: two new families related to disease resistance
RT TIR-NBS-LRR proteins encoded in Arabidopsis and other plant genomes.";
RL Plant J. 32:77-92(2002).
RN [5]
RP FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=23617639; DOI=10.1111/tpj.12219;
RA Zbierzak A.M., Porfirova S., Griebel T., Melzer M., Parker J.E.,
RA Doermann P.;
RT "A TIR-NBS protein encoded by Arabidopsis CHILLING SENSITIVE 1 (CHS1)
RT limits chloroplast damage and cell death at low temperature.";
RL Plant J. 75:539-552(2013).
CC -!- FUNCTION: Confers resistance to low temperatures by limiting
CC chloroplast damage and cell death, thus maintaining growth homeostasis.
CC {ECO:0000269|PubMed:23617639}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + NAD(+) = ADP-D-ribose + H(+) + nicotinamide;
CC Xref=Rhea:RHEA:16301, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17154, ChEBI:CHEBI:57540, ChEBI:CHEBI:57967; EC=3.2.2.6;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00204};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16302;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00204};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:F4I902}.
CC -!- TISSUE SPECIFICITY: Mostly expressed in leaves, stems and roots, and,
CC to a lower extent, in flowers and siliques.
CC {ECO:0000269|PubMed:23617639}.
CC -!- DOMAIN: The TIR domain mediates NAD(+) hydrolase (NADase) activity.
CC Self-association of TIR domains is required for NADase activity.
CC {ECO:0000255|PROSITE-ProRule:PRU00204}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAY56443.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AB022222; BAA97356.1; -; Genomic_DNA.
DR EMBL; CP002688; AED94509.1; -; Genomic_DNA.
DR EMBL; BT023452; AAY56443.1; ALT_INIT; mRNA.
DR EMBL; BT029233; ABJ98565.1; -; mRNA.
DR RefSeq; NP_198825.3; NM_123372.4.
DR AlphaFoldDB; Q9LUJ8; -.
DR SMR; Q9LUJ8; -.
DR STRING; 3702.AT5G40090.1; -.
DR PaxDb; Q9LUJ8; -.
DR PRIDE; Q9LUJ8; -.
DR ProteomicsDB; 246995; -.
DR EnsemblPlants; AT5G40090.1; AT5G40090.1; AT5G40090.
DR GeneID; 834006; -.
DR Gramene; AT5G40090.1; AT5G40090.1; AT5G40090.
DR KEGG; ath:AT5G40090; -.
DR Araport; AT5G40090; -.
DR TAIR; locus:2174915; AT5G40090.
DR HOGENOM; CLU_001561_2_3_1; -.
DR InParanoid; Q9LUJ8; -.
DR OMA; SFCCETS; -.
DR OrthoDB; 1041391at2759; -.
DR PhylomeDB; Q9LUJ8; -.
DR PRO; PR:Q9LUJ8; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LUJ8; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0043531; F:ADP binding; IEA:InterPro.
DR GO; GO:0050135; F:NAD(P)+ nucleosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0061809; F:NAD+ nucleotidase, cyclic ADP-ribose generating; IEA:UniProtKB-EC.
DR GO; GO:0006952; P:defense response; IEA:InterPro.
DR GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR Gene3D; 1.10.8.430; -; 1.
DR Gene3D; 3.40.50.10140; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR042197; Apaf_helical.
DR InterPro; IPR044974; Disease_R_plants.
DR InterPro; IPR002182; NB-ARC.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000157; TIR_dom.
DR InterPro; IPR035897; Toll_tir_struct_dom_sf.
DR PANTHER; PTHR11017; PTHR11017; 1.
DR Pfam; PF00931; NB-ARC; 1.
DR Pfam; PF01582; TIR; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52200; SSF52200; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS50104; TIR; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Hydrolase; NAD; Reference proteome.
FT CHAIN 1..459
FT /note="Disease resistance protein CHL1"
FT /id="PRO_0000438138"
FT DOMAIN 16..170
FT /note="TIR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00204"
FT DOMAIN 191..401
FT /note="NB-ARC"
FT /evidence="ECO:0000255"
FT REGION 429..459
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 89
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00204"
FT CONFLICT 51
FT /note="L -> S (in Ref. 3; AAY56443)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 459 AA; 51655 MW; 3FF27BB006EC3ACF CRC64;
MSSASSSSAA SLLLGREVDV FLSFCCQTSH GYFQNILIRN GIRTFLSYRC LEKRFGPIGQ
RTLKALEESR VAVVMTSTTK PCSVGFLEEL LVILEFQEKG SLMVIPIFLT DLSFNVEEIC
RQHPEKAPSW RTALTKLTNL AAEYPLSQNL AGMDQSDLLN QIARDISLVV FYSGSNDSNA
LVAMDRHMKV VYDLLALEVN KEVRTIGIWG SAGVGKTTLA RYIYAEIFVN FQTHVFLDNV
ENMKDKLLKF EGEEDPTVII SSYHDGHEIT EARRKHRKIL LIADDVNNME QGKWIIEYAN
WFAPGSRVIL ISQNKNLLVD AGVMDVYEVR SLRYDEALQV FSHFAFKQPY PPSDFEELAV
RAVHLAGFLP LGLRLLGSFL AGKGREEWVA ALLKLKAKQG GHIMEVWKLM EATDDKGLEE
WETAADIVER KESSQDKSQQ ESEVAADILI GKESSQDKQ
