ID   CHLE2_BRALA             Reviewed;         337 AA.
AC   Q95001;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=Cholinesterase 2;
DE            EC=3.1.1.8;
DE   Flags: Fragment;
GN   Name=CHE2;
OS   Branchiostoma lanceolatum (Common lancelet) (Amphioxus lanceolatum).
OC   Eukaryota; Metazoa; Chordata; Cephalochordata; Leptocardii; Amphioxiformes;
OC   Branchiostomidae; Branchiostoma.
OX   NCBI_TaxID=7740;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9062997;
RX   DOI=10.1002/(sici)1097-010x(19970215)277:3<213::aid-jez3>3.0.co;2-r;
RA   Sutherland D., McClellan J.S., Milner D., Soong W., Axon N., Sanders M.,
RA   Hester A., Kao Y.H., Poczatek T., Routt S., Pezzementi L.;
RT   "Two cholinesterase activities and genes are present in amphioxus.";
RL   J. Exp. Zool. 277:213-229(1997).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an acylcholine + H2O = a carboxylate + choline + H(+);
CC         Xref=Rhea:RHEA:21964, ChEBI:CHEBI:15354, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:29067, ChEBI:CHEBI:35287; EC=3.1.1.8;
CC   -!- SIMILARITY: Belongs to the type-B carboxylesterase/lipase family.
CC       {ECO:0000305}.
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DR   EMBL; U74379; AAB18263.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q95001; -.
DR   SMR; Q95001; -.
DR   MEROPS; S09.980; -.
DR   GO; GO:0004104; F:cholinesterase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR002018; CarbesteraseB.
DR   InterPro; IPR019826; Carboxylesterase_B_AS.
DR   InterPro; IPR000997; Cholinesterase.
DR   Pfam; PF00135; COesterase; 1.
DR   PRINTS; PR00878; CHOLNESTRASE.
DR   SUPFAM; SSF53474; SSF53474; 1.
DR   PROSITE; PS00122; CARBOXYLESTERASE_B_1; 1.
PE   3: Inferred from homology;
KW   Disulfide bond; Glycoprotein; Hydrolase; Serine esterase.
FT   CHAIN           <1..>337
FT                   /note="Cholinesterase 2"
FT                   /id="PRO_0000070290"
FT   ACT_SITE        99
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10039"
FT   ACT_SITE        224
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        290
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        153..165
FT                   /evidence="ECO:0000250"
FT   NON_TER         1
FT   NON_TER         337
SQ   SEQUENCE   337 AA;  36738 MW;  C9DE20A316C71258 CRC64;
     QPSPVPVGAT VMVWIYGGGF MSGTASLDVY DGRYIAATQG VIVASMNYRT GAMGFLSLGN
     SEAPGNAGLM DQNLALTWIK ENVASFGGDQ SKVSIFGESA GAASVSYHLL SPMSKNLFQR
     AIMESASALS PWALLPDAEA HRRGVELAKA VGCSTDSDIE ETIECMRGVP ALTISENEWV
     VWGLCQFPFA PVVDGNFIRE HPTVSLQTGN LKQTDVMVGF NNDEGVYFLL YGAPGFSKDT
     QSLITRDQYL EGIKMSVMGI NDISVDALSF QYIDWVNFDQ PSMYRDAIDN LSGDYNFICP
     ALSFGKAMAS FMGRKTYQYK FVHQASNFPW PKWTGVM