ID   CHLEP_MYCTO             Reviewed;         413 AA.
AC   P9WJX6; L0T4M5; O07435; Q7DAA8;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 30.
DE   RecName: Full=Chloramphenicol efflux pump MT0201 {ECO:0000250|UniProtKB:P9WJX7};
GN   OrderedLocusNames=MT0201;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Active efflux pump that plays an important role in
CC       chloramphenicol resistance. {ECO:0000250|UniProtKB:P9WJX7}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:P9WJX7};
CC       Multi-pass membrane protein {ECO:0000255}.
CC   -!- INDUCTION: Expression is negatively regulated by the transcriptional
CC       repressor MT1396. {ECO:0000250|UniProtKB:P9WJX7}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC       {ECO:0000305}.
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DR   EMBL; AE000516; AAK44421.1; -; Genomic_DNA.
DR   PIR; B70907; B70907.
DR   RefSeq; WP_010924196.1; NC_002755.2.
DR   AlphaFoldDB; P9WJX6; -.
DR   SMR; P9WJX6; -.
DR   EnsemblBacteria; AAK44421; AAK44421; MT0201.
DR   KEGG; mtc:MT0201; -.
DR   HOGENOM; CLU_001265_61_1_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.1250.20; -; 2.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; Cell membrane; Membrane; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..413
FT                   /note="Chloramphenicol efflux pump MT0201"
FT                   /id="PRO_0000427753"
FT   TRANSMEM        23..43
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        55..75
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        89..109
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        110..130
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        150..170
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        176..196
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        226..246
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        256..276
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        286..306
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        312..332
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        353..373
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        378..398
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   413 AA;  42871 MW;  75CC3CB69FB06CA3 CRC64;
     MTAPTGTSAT TTRPWTPRIA TQLSVLACAA FIYVTAEILP VGALSAIARN LRVSVVLVGT
     LLSWYALVAA VTTVPLVRWT AHWPRRRALV VSLVCLTVSQ LVSALAPNFA VLAAGRVLCA
     VTHGLLWAVI APIATRLVPP SHAGRATTSI YIGTSLALVV GSPLTAAMSL MWGWRLAAVC
     VTGAAAAVAL AARLALPEMV LRADQLEHVG RRARHPRNPR LVKVSVLTMI AVTGHFVSYT
     YIVVIIRDVV GVRGPNLAWL LAAYGVAGLV SVPLVARPLD RWPKGAVIVG MTGLTAAFTL
     LTALAFGERH TAATALLGTG AIVLWGALAT AVSPMLQSAA MRSGGDDPDG ASGLYVTAFQ
     IGIMAGALLG GLLYERSLAM MLTASAGLMG VALFGMTVSQ HLFENPTLSP GDG