CHLE_BOVIN
ID CHLE_BOVIN Reviewed; 602 AA.
AC P32749; Q08DR6;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 03-APR-2007, sequence version 2.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Cholinesterase;
DE EC=3.1.1.8;
DE AltName: Full=Acylcholine acylhydrolase;
DE AltName: Full=Butyrylcholine esterase;
DE AltName: Full=Choline esterase II;
DE AltName: Full=Pseudocholinesterase;
DE Flags: Precursor;
GN Name=BCHE;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Hereford; TISSUE=Fetal cerebellum;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 96-236.
RC TISSUE=Lung;
RX PubMed=2016308; DOI=10.1016/s0021-9258(20)89597-0;
RA Arpagaus M., Chatonnet A., Masson P., Newton M., Vaughan T.A.,
RA Bartels C.F., Nogueira C.P., la Du B.N., Lockridge O.;
RT "Use of the polymerase chain reaction for homology probing of
RT butyrylcholinesterase from several vertebrates.";
RL J. Biol. Chem. 266:6966-6974(1991).
CC -!- FUNCTION: Esterase with broad substrate specificity. Contributes to the
CC inactivation of the neurotransmitter acetylcholine. Can degrade
CC neurotoxic organophosphate esters (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an acylcholine + H2O = a carboxylate + choline + H(+);
CC Xref=Rhea:RHEA:21964, ChEBI:CHEBI:15354, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:29067, ChEBI:CHEBI:35287; EC=3.1.1.8;
CC -!- SUBUNIT: Homotetramer; disulfide-linked. Dimer of dimers (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Present in most cells except erythrocytes.
CC -!- SIMILARITY: Belongs to the type-B carboxylesterase/lipase family.
CC {ECO:0000305}.
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DR EMBL; BC123600; AAI23601.1; -; mRNA.
DR EMBL; M62410; AAA51412.1; -; Genomic_DNA.
DR PIR; F39768; F39768.
DR RefSeq; NP_001070374.1; NM_001076906.1.
DR AlphaFoldDB; P32749; -.
DR SMR; P32749; -.
DR STRING; 9913.ENSBTAP00000014794; -.
DR ESTHER; bovin-BCHE; BCHE.
DR MEROPS; S09.980; -.
DR PaxDb; P32749; -.
DR PRIDE; P32749; -.
DR Ensembl; ENSBTAT00000068356; ENSBTAP00000068076; ENSBTAG00000011139.
DR GeneID; 534616; -.
DR KEGG; bta:534616; -.
DR CTD; 590; -.
DR VEuPathDB; HostDB:ENSBTAG00000011139; -.
DR VGNC; VGNC:26443; BCHE.
DR eggNOG; KOG4389; Eukaryota.
DR GeneTree; ENSGT00940000157023; -.
DR HOGENOM; CLU_006586_13_0_1; -.
DR InParanoid; P32749; -.
DR OMA; EMRYICP; -.
DR OrthoDB; 754103at2759; -.
DR TreeFam; TF315470; -.
DR BRENDA; 3.1.1.8; 908.
DR Proteomes; UP000009136; Chromosome 1.
DR Bgee; ENSBTAG00000011139; Expressed in duodenum and 97 other tissues.
DR ExpressionAtlas; P32749; baseline and differential.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0003990; F:acetylcholinesterase activity; ISS:UniProtKB.
DR GO; GO:0052689; F:carboxylic ester hydrolase activity; IBA:GO_Central.
DR GO; GO:0004104; F:cholinesterase activity; ISS:UniProtKB.
DR GO; GO:0006581; P:acetylcholine catabolic process; IBA:GO_Central.
DR GO; GO:0019695; P:choline metabolic process; IBA:GO_Central.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR014788; AChE_tetra.
DR InterPro; IPR002018; CarbesteraseB.
DR InterPro; IPR019826; Carboxylesterase_B_AS.
DR InterPro; IPR019819; Carboxylesterase_B_CS.
DR InterPro; IPR000997; Cholinesterase.
DR Pfam; PF08674; AChE_tetra; 1.
DR Pfam; PF00135; COesterase; 1.
DR PRINTS; PR00878; CHOLNESTRASE.
DR SUPFAM; SSF53474; SSF53474; 1.
DR PROSITE; PS00122; CARBOXYLESTERASE_B_1; 1.
DR PROSITE; PS00941; CARBOXYLESTERASE_B_2; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Glycoprotein; Hydrolase; Phosphoprotein;
KW Reference proteome; Secreted; Serine esterase; Signal.
FT SIGNAL 1..28
FT /evidence="ECO:0000255"
FT CHAIN 29..602
FT /note="Cholinesterase"
FT /id="PRO_0000070283"
FT ACT_SITE 226
FT /note="Acyl-ester intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10039"
FT ACT_SITE 353
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 466
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT BINDING 144..145
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT MOD_RES 226
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P06276"
FT CARBOHYD 85
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 134
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 269
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 284
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 369
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 483
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 509
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 513
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 514
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 602 AA; 68867 MW; CDFB404E64C46935 CRC64;
MQSRSTVIYI RFVLWFLLLW VLFEKSHTEE DIIITTKNGK VRGMHLPVLG GTVTAFLGIP
YAQPPLGRLR FKKPQSLTKW PDIWNATKYA NSCYQNTDQS FPGFLGSEMW NPNTDLSEDC
LYLNVWIPTP KPKNATVMIW IYGGSFQTGT SSLHVYDGKF LARVERVIVV SMNYRVGALG
FLALPGNPEA PGNVGLFDQQ LALQWVQKNI AAFGGNPKSV TLFGESAGAA SVSLHLLSPE
SHPLFTRAIL QSGSSNAPWA VTSRYEARNR TLTLAKFIGC SRENDTEIIK CLRNKDPQEI
LRHEVFVVPY GTLLSVNFGP TVDGDFLTDM PDTLLQLGQF KKTQILVGVN KDEGTAFLVY
GAPGFSKDNN SIITRKEFQE GLKIFFPGVS EFGKESILFH YMDWLDDQRA EKYREALDDV
VGDYNIICPA LEFTKKFSDM GNNAFFYYFE HRSSKLPWPE WMGVMHGYEI EFVFGLPLER
RVNYTKAEEI FSRSIMKRWA NFAKYGNPNG TQNNSTRWPV FKSNEQKYFT LNTESPKVNT
KLRAQQCRFW TLFFPKVLEI TGNIDEVERE WKAGFHRWNN YMMDWKNQFN DYTSKKESCA
GL
