CHLG_ARATH
ID CHLG_ARATH Reviewed; 387 AA.
AC Q38833;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Chlorophyll synthase, chloroplastic;
DE EC=2.5.1.62;
DE AltName: Full=Polyprenyl transferase;
DE AltName: Full=Protein G4;
DE Short=AtG4;
DE Flags: Precursor;
GN Name=CHLG; Synonyms=G4; OrderedLocusNames=At3g51820; ORFNames=ATEM1.7;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=8552034; DOI=10.1007/bf00290236;
RA Gaubier P., Wu H.-J., Laudie M., Delseny M., Grellet F.;
RT "A chlorophyll synthetase gene from Arabidopsis thaliana.";
RL Mol. Gen. Genet. 249:58-64(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10645728; DOI=10.1023/a:1006395324818;
RA Comella P., Wu H.-J., Laudie M., Berger C., Cooke R., Delseny M.,
RA Grellet F.;
RT "Fine sequence analysis of 60 kb around the Arabidopsis thaliana AtEm1
RT locus on chromosome III.";
RL Plant Mol. Biol. 41:687-700(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP FUNCTION.
RA Oster U., Ruediger W.;
RT "The G4 gene of Arabidopsis thaliana encodes a chlorophyll synthase of
RT etiolated plants.";
RL Bot. Acta 110:420-423(1997).
CC -!- FUNCTION: Involved in one of the last steps of the biosynthesis of
CC chlorophyll a. Catalyzes the esterification of chlorophillide a or b
CC with a preference for geranylgeranyldiphosphate (GGPP) rather than for
CC phytyldiphosphate (PhyPP). {ECO:0000269|Ref.5}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=chlorophyllide a + H(+) + phytyl diphosphate = chlorophyll a +
CC diphosphate; Xref=Rhea:RHEA:17317, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:58416, ChEBI:CHEBI:75434,
CC ChEBI:CHEBI:83348; EC=2.5.1.62;
CC -!- PATHWAY: Porphyrin-containing compound metabolism; chlorophyll
CC biosynthesis.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000305};
CC Multi-pass membrane protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Low level in flower buds, flowers, stems, leaves,
CC greening cotyledons and immature siliques, but not in mature siliques
CC or seeds. {ECO:0000269|PubMed:8552034}.
CC -!- SIMILARITY: Belongs to the UbiA prenyltransferase family. Chlorophyll
CC synthase subfamily. {ECO:0000305}.
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DR EMBL; U19382; AAA96740.1; -; Genomic_DNA.
DR EMBL; AF049236; AAC14409.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE78848.1; -; Genomic_DNA.
DR EMBL; AY081481; AAM10043.1; -; mRNA.
DR EMBL; AY042821; AAK68761.1; -; mRNA.
DR PIR; S60222; S60222.
DR RefSeq; NP_190750.1; NM_115041.4.
DR AlphaFoldDB; Q38833; -.
DR SMR; Q38833; -.
DR BioGRID; 9663; 2.
DR STRING; 3702.AT3G51820.1; -.
DR PaxDb; Q38833; -.
DR PRIDE; Q38833; -.
DR ProteomicsDB; 245177; -.
DR EnsemblPlants; AT3G51820.1; AT3G51820.1; AT3G51820.
DR GeneID; 824345; -.
DR Gramene; AT3G51820.1; AT3G51820.1; AT3G51820.
DR KEGG; ath:AT3G51820; -.
DR Araport; AT3G51820; -.
DR TAIR; locus:2074393; AT3G51820.
DR eggNOG; ENOG502QQMP; Eukaryota.
DR HOGENOM; CLU_042598_1_0_1; -.
DR InParanoid; Q38833; -.
DR OMA; QDMYFLR; -.
DR OrthoDB; 1231215at2759; -.
DR PhylomeDB; Q38833; -.
DR BioCyc; ARA:AT3G51820-MON; -.
DR BioCyc; MetaCyc:AT3G51820-MON; -.
DR BRENDA; 2.5.1.62; 399.
DR UniPathway; UPA00668; -.
DR PRO; PR:Q38833; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q38833; baseline and differential.
DR Genevisible; Q38833; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009534; C:chloroplast thylakoid; HDA:TAIR.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0046408; F:chlorophyll synthetase activity; IEA:UniProtKB-EC.
DR GO; GO:0015995; P:chlorophyll biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd13958; PT_UbiA_chlorophyll; 1.
DR Gene3D; 1.10.357.140; -; 1.
DR InterPro; IPR006372; Chl_synth.
DR InterPro; IPR011799; ChlG.
DR InterPro; IPR000537; UbiA_prenyltransferase.
DR InterPro; IPR044878; UbiA_sf.
DR Pfam; PF01040; UbiA; 1.
DR TIGRFAMs; TIGR02056; ChlG; 1.
DR TIGRFAMs; TIGR01476; chlor_syn_BchG; 1.
PE 2: Evidence at transcript level;
KW Chlorophyll biosynthesis; Chloroplast; Membrane; Plastid;
KW Reference proteome; Transferase; Transit peptide; Transmembrane;
KW Transmembrane helix.
FT TRANSIT 1..57
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 58..387
FT /note="Chlorophyll synthase, chloroplastic"
FT /id="PRO_0000285115"
FT TRANSMEM 182..202
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 210..230
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 241..261
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 266..286
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 311..331
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 336..356
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 364..384
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 62..81
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 387 AA; 41881 MW; 518080F2BE018D29 CRC64;
MTSILNTVST IHSSRVTSVD RVGVLSLRNS DSVEFTRRRS GFSTLIYESP GRRFVVRAAE
TDTDKVKSQT PDKAPAGGSS INQLLGIKGA SQETNKWKIR LQLTKPVTWP PLVWGVVCGA
AASGNFHWTP EDVAKSILCM MMSGPCLTGY TQTINDWYDR DIDAINEPYR PIPSGAISEP
EVITQVWVLL LGGLGIAGIL DVWAGHTTPT VFYLALGGSL LSYIYSAPPL KLKQNGWVGN
FALGASYISL PWWAGQALFG TLTPDVVVLT LLYSIAGLGI AIVNDFKSVE GDRALGLQSL
PVAFGTETAK WICVGAIDIT QLSVAGYLLA SGKPYYALAL VALIIPQIVF QFKYFLKDPV
KYDVKYQASA QPFLVLGIFV TALASQH
