ID   CHLH_ORYSI              Reviewed;        1387 AA.
AC   B8ANF1;
DT   05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 1.
DT   25-MAY-2022, entry version 52.
DE   RecName: Full=Magnesium-chelatase subunit ChlH, chloroplastic;
DE            Short=Mg-chelatase subunit H;
DE            EC=6.6.1.1;
DE   AltName: Full=Mg-protoporphyrin IX chelatase subunit ChlH;
DE   Flags: Precursor;
GN   Name=CHLH; ORFNames=OsI_11362;
OS   Oryza sativa subsp. indica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39946;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. 93-11;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
CC   -!- FUNCTION: Involved in chlorophyll biosynthesis. Catalyzes the insertion
CC       of magnesium ion into protoporphyrin IX to yield Mg-protoporphyrin IX.
CC       The reaction takes place in two steps, with an ATP-dependent activation
CC       followed by an ATP-dependent chelation step. May be involved in the
CC       plastid-to-nucleus retrograde signaling (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O + Mg(2+) + protoporphyrin IX = ADP + 3 H(+) + Mg-
CC         protoporphyrin IX + phosphate; Xref=Rhea:RHEA:13961,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:18420,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:57306,
CC         ChEBI:CHEBI:60492, ChEBI:CHEBI:456216; EC=6.6.1.1;
CC   -!- PATHWAY: Porphyrin-containing compound metabolism; chlorophyll
CC       biosynthesis.
CC   -!- SUBUNIT: The magnesium chelatase complex is a heterotrimer consisting
CC       of subunits CHLI, CHLD, AND CHLH. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma {ECO:0000250}.
CC       Plastid, chloroplast membrane {ECO:0000250}; Peripheral membrane
CC       protein {ECO:0000250}; Stromal side {ECO:0000250}. Plastid, chloroplast
CC       membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250};
CC       Cytoplasmic side {ECO:0000250}. Note=Predominantly associated with the
CC       chloroplast envelope. Spans the chloroplast envelope and its N- and C-
CC       termini are exposed to the cytosol (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mg-chelatase subunit H family.
CC       {ECO:0000305}.
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DR   EMBL; CM000128; EEC75155.1; -; Genomic_DNA.
DR   AlphaFoldDB; B8ANF1; -.
DR   SMR; B8ANF1; -.
DR   STRING; 39946.B8ANF1; -.
DR   EnsemblPlants; BGIOSGA012530-TA; BGIOSGA012530-PA; BGIOSGA012530.
DR   Gramene; BGIOSGA012530-TA; BGIOSGA012530-PA; BGIOSGA012530.
DR   HOGENOM; CLU_002017_1_2_1; -.
DR   OMA; WETGQAM; -.
DR   UniPathway; UPA00668; -.
DR   Proteomes; UP000007015; Chromosome 3.
DR   GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0009570; C:chloroplast stroma; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016851; F:magnesium chelatase activity; IEA:UniProtKB-EC.
DR   GO; GO:0015995; P:chlorophyll biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR   CDD; cd10150; CobN_like; 1.
DR   InterPro; IPR011771; BchH.
DR   InterPro; IPR003672; CobN/Mg_chltase.
DR   InterPro; IPR022571; Mg_chelatase_H_N.
DR   PANTHER; PTHR44119; PTHR44119; 1.
DR   Pfam; PF02514; CobN-Mg_chel; 1.
DR   Pfam; PF11965; DUF3479; 1.
DR   TIGRFAMs; TIGR02025; BchH; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Chlorophyll biosynthesis; Chloroplast; Ligase; Membrane;
KW   Nucleotide-binding; Photosynthesis; Plastid; Reference proteome;
KW   Transit peptide.
FT   TRANSIT         1..50
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           51..1387
FT                   /note="Magnesium-chelatase subunit ChlH, chloroplastic"
FT                   /id="PRO_0000418767"
SQ   SEQUENCE   1387 AA;  153412 MW;  7037904BB4895B89 CRC64;
     MSSLVSTPFT TATGVQKKLG APVPLHSFLL SRRQPAAGAG RGRAAAAAIR CAVAGNGLFT
     QTKPEVRRVV PPEGDASRRG VPRVKVVYVV LEAQYQSSVT AAVRELNADP RRAAGFEVVG
     YLVEELRDEE TYKTFCADLA DANVFIGSLI FVEELALKVK DAVEKERDRM DAVLVFPSMP
     EVMRLNKLGS FSMSQLGQSK SPFFQLFKRK KNSGGFADSM LKLVRTLPKV LKYLPSDKAQ
     DARLYILSLQ FWLGGSPDNL QNFLKMIAVS YVPALKGADI KYDDPVLFLD AGIWHPLAPT
     MYDDVKEYLN WYGTRRDTND KLKDPNAPVI GLVLQRSHIV TGDDGHYVAV IMELEAKGAK
     VIPIFAGGLD FSGPTQRYLV DPITGKPFVN AVVSLTGFAL VGGPARQDHP KAIAALQKLD
     VPYIVALPLV FQTTEEWLNS TLGLHPIQVA LQVALPELDG GMEPIVFAGR DPRTGKSHAL
     HKRVEQLCTR AIRWAELKRK TKEEKKLAIT VFSFPPDKGN VGTAAYLNVF NSIYSVLQDL
     KKDGYNVEGL PDTAEALIEE VIHDKEAQFN SPNLNVAYRM NVREYQSLTS YASLLEENWG
     KPPGNLNSDG ENLLVYGKQY GNVFIGVQPT FGYEGDPMRL LFSKSASPHH GFAAYYTFVE
     KIFQADAVLH FGTHGSLEFM PGKQVGMSDA CYPDSLIGNI PNIYYYAANN PSEATVAKRR
     SYANTISYLT PPAENAGLYK GLKQLSELIS SYQSLKDTGR GPQIVSSIIS TAKQCNLDKD
     VPLPEEGVEL PPNERDLIVG KVYAKIMEIE SRLLPCGLHV IGEPPSAIEA VATLVNIASL
     DRPEDEIYSL PNILAQTVGR NIEDVYRGSD KGILADVELL RQITEASRGA ITAFVERTTN
     NKGQVVDVTN KLSTMLGFGL SEPWVQHLSK TKFIRADREK LRTLFTFLGE CLKLIVADNE
     LGSLKLALEG SYVEPGPGGD PIRNPKVLPT GKNIHALDPQ AIPTTAALKS AKIVVDRLLE
     RQKVDNGGKY PETIALVLWG TDNIKTYGES LAQVLWMIGV RPVADTFGRV NRVEPVSLEE
     LGRPRIDVVV NCSGVFRDLF INQMNLLDRA VKMVAELDEP EEMNYVRKHA QEQARELGVS
     LREAATRVFS NASGSYSSNV NLAVENASWT DEKQLQDMYL SRKSFAFDCD APGAGMREQR
     KTFELALATA DATFQNLDSS EISLTDVSHY FDSDPTKLVQ GLRKDGRAPS SYIADTTTAN
     AQVRTLSETV RLDARTKLLN PKWYEGMMKS GYEGVREIEK RLTNTVGWSA TSGQVDNWVY
     EEANATFIED EAMRKRLMDT NPNSFRKLVQ TFLEASGRGY WETSEENLEK LRELYSEVED
     KIEGIDR