ID   CHLH_ORYSJ              Reviewed;        1387 AA.
AC   Q10M50; Q0DSA2;
DT   05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT   22-AUG-2006, sequence version 1.
DT   03-AUG-2022, entry version 77.
DE   RecName: Full=Magnesium-chelatase subunit ChlH, chloroplastic;
DE            Short=Mg-chelatase subunit H;
DE            EC=6.6.1.1;
DE   AltName: Full=Mg-protoporphyrin IX chelatase subunit ChlH;
DE   Flags: Precursor;
GN   Name=CHLH; OrderedLocusNames=Os03g0323200, LOC_Os03g20700;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16109971; DOI=10.1101/gr.3869505;
RG   The rice chromosome 3 sequencing consortium;
RA   Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA   Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA   Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA   Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA   Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA   Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA   Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA   Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA   Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA   Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA   O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA   Jin W., Lee H.R., Jiang J., Jackson S.;
RT   "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT   and diverged grass species.";
RL   Genome Res. 15:1284-1291(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   FUNCTION, AND SUBUNIT.
RX   PubMed=22226678; DOI=10.1016/j.febslet.2011.12.026;
RA   Zhou S., Sawicki A., Willows R.D., Luo M.;
RT   "C-terminal residues of oryza sativa GUN4 are required for the activation
RT   of the ChlH subunit of magnesium chelatase in chlorophyll synthesis.";
RL   FEBS Lett. 586:205-210(2012).
CC   -!- FUNCTION: Involved in chlorophyll biosynthesis. Catalyzes the insertion
CC       of magnesium ion into protoporphyrin IX to yield Mg-protoporphyrin IX.
CC       The reaction takes place in two steps, with an ATP-dependent activation
CC       followed by an ATP-dependent chelation step. May be involved in the
CC       plastid-to-nucleus retrograde signaling. {ECO:0000269|PubMed:22226678}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O + Mg(2+) + protoporphyrin IX = ADP + 3 H(+) + Mg-
CC         protoporphyrin IX + phosphate; Xref=Rhea:RHEA:13961,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:18420,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:57306,
CC         ChEBI:CHEBI:60492, ChEBI:CHEBI:456216; EC=6.6.1.1;
CC   -!- PATHWAY: Porphyrin-containing compound metabolism; chlorophyll
CC       biosynthesis.
CC   -!- SUBUNIT: The magnesium chelatase complex is a heterotrimer consisting
CC       of subunits CHLI, CHLD, AND CHLH. {ECO:0000269|PubMed:22226678}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma {ECO:0000250}.
CC       Plastid, chloroplast membrane {ECO:0000250}; Peripheral membrane
CC       protein {ECO:0000250}; Stromal side {ECO:0000250}. Plastid, chloroplast
CC       membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250};
CC       Cytoplasmic side {ECO:0000250}. Note=Predominantly associated with the
CC       chloroplast envelope. Spans the chloroplast envelope and its N- and C-
CC       termini are exposed to the cytosol (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mg-chelatase subunit H family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAF11886.2; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; DP000009; ABF95686.1; -; Genomic_DNA.
DR   EMBL; DP000009; ABF95687.1; -; Genomic_DNA.
DR   EMBL; AP008209; BAF11886.2; ALT_INIT; Genomic_DNA.
DR   EMBL; AP014959; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   RefSeq; XP_015631165.1; XM_015775679.1.
DR   RefSeq; XP_015631166.1; XM_015775680.1.
DR   AlphaFoldDB; Q10M50; -.
DR   SMR; Q10M50; -.
DR   STRING; 4530.OS03T0323200-02; -.
DR   PaxDb; Q10M50; -.
DR   PRIDE; Q10M50; -.
DR   KEGG; osa:4332690; -.
DR   eggNOG; ENOG502QT3B; Eukaryota.
DR   HOGENOM; CLU_002017_1_1_1; -.
DR   InParanoid; Q10M50; -.
DR   OrthoDB; 64867at2759; -.
DR   UniPathway; UPA00668; -.
DR   Proteomes; UP000000763; Chromosome 3.
DR   Proteomes; UP000059680; Chromosome 3.
DR   Genevisible; Q10M50; OS.
DR   GO; GO:0009507; C:chloroplast; IBA:GO_Central.
DR   GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0009570; C:chloroplast stroma; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016851; F:magnesium chelatase activity; IEA:UniProtKB-EC.
DR   GO; GO:0015995; P:chlorophyll biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR   CDD; cd10150; CobN_like; 1.
DR   InterPro; IPR011771; BchH.
DR   InterPro; IPR003672; CobN/Mg_chltase.
DR   InterPro; IPR022571; Mg_chelatase_H_N.
DR   PANTHER; PTHR44119; PTHR44119; 1.
DR   Pfam; PF02514; CobN-Mg_chel; 1.
DR   Pfam; PF11965; DUF3479; 1.
DR   TIGRFAMs; TIGR02025; BchH; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Chlorophyll biosynthesis; Chloroplast; Ligase; Membrane;
KW   Nucleotide-binding; Photosynthesis; Plastid; Reference proteome;
KW   Transit peptide.
FT   TRANSIT         1..50
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           51..1387
FT                   /note="Magnesium-chelatase subunit ChlH, chloroplastic"
FT                   /id="PRO_0000418766"
SQ   SEQUENCE   1387 AA;  153470 MW;  E453AE49A130FA5F CRC64;
     MSSLVSTPFT TATGVQKKLG APVPLHSFLL SRRQPAAGAG RGRAAAAAIR CAVAGNGLFT
     QTKPEVRRVV PPEGDASRRG VPRVKVVYVV LEAQYQSSVT AAVRELNADP RRAAGFEVVG
     YLVEELRDEE TYKTFCADLA DANVFIGSLI FVEELALKVK DAVEKERDRM DAVLVFPSMP
     EVMRLNKLGS FSMSQLGQSK SPFFQLFKRK KNSGGFADSM LKLVRTLPKV LKYLPSDKAQ
     DARLYILSLQ FWLGGSPDNL QNFLKMIAVS YVPALKGADI KYDDPVLFLD AGIWHPLAPT
     MYDDVKEYLN WYGTRRDTND KLKDPNAPVI GLVLQRSHIV TGDDGHYVAV IMELEAKGAK
     VIPIFAGGLD FSGPTQRYLV DPITGKPFVN AVVSLTGFAL VGGPARQDHP KAIAALQKLD
     VPYIVALPLV FQTTEEWLNS TLGLHPIQVA LQVALPELDG GMEPIVFAGR DPRTGKSHAL
     HKRVEQLCTR AIRWAELKRK TKEEKKLAIT VFSFPPDKGN VGTAAYLNVF NSIYSVLQDL
     KKDGYNVEGL PDTAEALIEE VIHDKEAQFN SPNLNVAYRM NVREYQSLTS YASLLEENWG
     KPPGNLNSDG ENLLVYGKQY GNVFIGVQPT FGYEGDPMRL LFSKSASPHH GFAAYYTFVE
     KIFQADAVLH FGTHGSLEFM PGKQVGMSDA CYPDSLIGNI PNIYYYAANN PSEATVAKRR
     SYANTISYLT PPAENAGLYK GLKQLSELIS SYQSLKDTGR GPQIVSSIIS TAKQCNLDKD
     VPLPEEGVEL PPNERDLIVG KVYAKIMEIE SRLLPCGLHV IGEPPSAIEA VATLVNIASL
     DRPEDEIYSL PNILAQTVGR NIEDVYRGSD KGILADVELL RQITEASRGA ITTFVERTTN
     NKGQVVDVTN KLSTMLGFGL SEPWVQHLSK TKFIRADREK LRTLFTFLGE CLKLIVADNE
     LGSLKLALEG SYVEPGPGGD PIRNPKVLPT GKNIHALDPQ AIPTTAALKS AKIIVDRLLE
     RQKVDNGGKY PETIALVLWG TDNIKTYGES LAQVLWMIGV RPVADTFGRV NRVEPVSLEE
     LGRPRIDVVI NCSGVFRDLF INQMNLLDRA VKMVAELDEP EEMNYVRKHA QEQARELGVS
     LREAATRVFS NASGSYSSNV NLAVENASWT DEKQLQDMYL SRKSFAFDCD APGAGMREQR
     KTFELALATA DATFQNLDSS EISLTDVSHY FDSDPTKLVQ GLRKDGRAPS SYIADTTTAN
     AQVRTLSETV RLDARTKLLN PKWYEGMMKS GYEGVREIEK RLTNTVGWSA TSGQVDNWVY
     EEANATFIED EAMRKRLMDT NPNSFRKLVQ TFLEASGRGY WETSEENLEK LRELYSEVED
     KIEGIDR