ID   CHLI_EUGGR              Reviewed;         348 AA.
AC   P31205;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Magnesium-chelatase subunit ChlI;
DE            EC=6.6.1.1;
DE   AltName: Full=Mg-protoporphyrin IX chelatase;
GN   Name=chlI; Synonyms=ccsA;
OS   Euglena gracilis.
OG   Plastid; Chloroplast.
OC   Eukaryota; Discoba; Euglenozoa; Euglenida; Spirocuta; Euglenophyceae;
OC   Euglenales; Euglenaceae; Euglena.
OX   NCBI_TaxID=3039;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Z / UTEX 753;
RX   PubMed=1607017; DOI=10.1016/0014-5793(92)80514-h;
RA   Orsat B., Monfort A., Chatellard P., Stutz E.;
RT   "Mapping and sequencing of an actively transcribed Euglena gracilis
RT   chloroplast gene (ccsA) homologous to the Arabidopsis thaliana nuclear gene
RT   cs(ch-42).";
RL   FEBS Lett. 303:181-184(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Z / UTEX 753;
RX   PubMed=8346031; DOI=10.1093/nar/21.15.3537;
RA   Hallick R.B., Hong L., Drager R.G., Favreau M.R., Monfort A., Orsat B.,
RA   Spielmann A., Stutz E.;
RT   "Complete sequence of Euglena gracilis chloroplast DNA.";
RL   Nucleic Acids Res. 21:3537-3544(1993).
CC   -!- FUNCTION: Involved in chlorophyll biosynthesis; introduces a magnesium
CC       ion into protoporphyrin IX to yield Mg-protoporphyrin IX.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O + Mg(2+) + protoporphyrin IX = ADP + 3 H(+) + Mg-
CC         protoporphyrin IX + phosphate; Xref=Rhea:RHEA:13961,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:18420,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:57306,
CC         ChEBI:CHEBI:60492, ChEBI:CHEBI:456216; EC=6.6.1.1;
CC   -!- PATHWAY: Porphyrin-containing compound metabolism; chlorophyll
CC       biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the Mg-chelatase subunits D/I family.
CC       {ECO:0000305}.
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DR   EMBL; Z11874; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; X65484; CAA46470.1; -; Genomic_DNA.
DR   EMBL; X70810; CAA50075.1; -; Genomic_DNA.
DR   PIR; S34494; S34494.
DR   RefSeq; NP_041888.1; NC_001603.2.
DR   AlphaFoldDB; P31205; -.
DR   SMR; P31205; -.
DR   GeneID; 807485; -.
DR   UniPathway; UPA00668; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016851; F:magnesium chelatase activity; IEA:UniProtKB-EC.
DR   GO; GO:0015995; P:chlorophyll biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR045006; CHLI-like.
DR   InterPro; IPR041628; ChlI/MoxR_AAA_lid.
DR   InterPro; IPR011775; Mg_chelatase_ATPase-isu.
DR   InterPro; IPR000523; Mg_chelatse_chII-like_cat_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR32039; PTHR32039; 1.
DR   Pfam; PF17863; AAA_lid_2; 1.
DR   Pfam; PF01078; Mg_chelatase; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR02030; BchI-ChlI; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Chlorophyll biosynthesis; Chloroplast; Ligase;
KW   Nucleotide-binding; Photosynthesis; Plastid.
FT   CHAIN           1..348
FT                   /note="Magnesium-chelatase subunit ChlI"
FT                   /id="PRO_0000206867"
FT   BINDING         42..49
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   348 AA;  39308 MW;  550CEE88E263D438 CRC64;
     MNKKTNERPV FPFTSIVGQE EMKLSLILNV IDPKIGGVMI MGDRGTGKST IVRALVDLLP
     PIDVIENDPY NSDPYDTELM SDDVLEKIKK NEKVSIIQVK TPMVDLPLGG TEDRVCGTID
     IEKAISEGKK AFEPGLLAQA NRGILYVDEV NLLDDHLVDV LLDSAASGWN TVEREGVSIC
     HPARFILVGS GNPEEGELRP QLLDRFGMHA QIKTLKEPAL RVKIVQQREL FEKSPKEFKE
     KYKEEQNKLM EKIINARKKL KNIIIKYELL EKISQICSEL NVDGLRGDMV TSRAAKALVA
     FEDRTEVTPK DIFTVITLCL RHRLRKDPLE SIDSGYKVQE TFKKVFNY