CHLI_ORYSJ
ID CHLI_ORYSJ Reviewed; 415 AA.
AC Q53RM0; A0A0P0VZB0;
DT 05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Magnesium-chelatase subunit ChlI, chloroplastic;
DE Short=Mg-chelatase subunit I;
DE EC=6.6.1.1;
DE AltName: Full=Mg-protoporphyrin IX chelatase subunit ChlI;
DE AltName: Full=Protein CHLORINA 9;
DE Flags: Precursor;
GN Name=CHLI; Synonyms=CHL9; OrderedLocusNames=Os03g0563300, LOC_Os03g36540;
GN ORFNames=OsJ_11451;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, SUBCELLULAR LOCATION,
RP SUBUNIT, AND MUTAGENESIS OF ARG-307.
RX PubMed=16915519; DOI=10.1007/s11103-006-9024-z;
RA Zhang H., Li J., Yoo J.H., Yoo S.C., Cho S.H., Koh H.J., Seo H.S.,
RA Paek N.C.;
RT "Rice Chlorina-1 and Chlorina-9 encode ChlD and ChlI subunits of Mg-
RT chelatase, a key enzyme for chlorophyll synthesis and chloroplast
RT development.";
RL Plant Mol. Biol. 62:325-337(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [8]
RP FUNCTION.
RX PubMed=22226678; DOI=10.1016/j.febslet.2011.12.026;
RA Zhou S., Sawicki A., Willows R.D., Luo M.;
RT "C-terminal residues of oryza sativa GUN4 are required for the activation
RT of the ChlH subunit of magnesium chelatase in chlorophyll synthesis.";
RL FEBS Lett. 586:205-210(2012).
CC -!- FUNCTION: Involved in chlorophyll biosynthesis. Catalyzes the insertion
CC of magnesium ion into protoporphyrin IX to yield Mg-protoporphyrin IX.
CC The reaction takes place in two steps, with an ATP-dependent activation
CC followed by an ATP-dependent chelation step.
CC {ECO:0000269|PubMed:16915519, ECO:0000269|PubMed:22226678}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O + Mg(2+) + protoporphyrin IX = ADP + 3 H(+) + Mg-
CC protoporphyrin IX + phosphate; Xref=Rhea:RHEA:13961,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:18420,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:57306,
CC ChEBI:CHEBI:60492, ChEBI:CHEBI:456216; EC=6.6.1.1;
CC -!- ACTIVITY REGULATION: Redox regulation; active in reducing conditions,
CC inactive in oxidizing conditions. Thioredoxins f and m mediate the
CC reversible reductive activation of oxidized CHLI (By similarity).
CC {ECO:0000250}.
CC -!- PATHWAY: Porphyrin-containing compound metabolism; chlorophyll
CC biosynthesis.
CC -!- SUBUNIT: The magnesium chelatase complex is a heterotrimer consisting
CC of subunits CHLI, CHLD, AND CHLH. {ECO:0000269|PubMed:16915519}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000305|PubMed:16915519}.
CC -!- SIMILARITY: Belongs to the Mg-chelatase subunits D/I family.
CC {ECO:0000305}.
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DR EMBL; EF065606; ABK58608.1; -; Genomic_DNA.
DR EMBL; EF065607; ABK58609.1; -; mRNA.
DR EMBL; AC084767; AAX95610.1; -; Genomic_DNA.
DR EMBL; DP000009; ABF97128.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF12407.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS84940.1; -; Genomic_DNA.
DR EMBL; CM000140; EAZ27502.1; -; Genomic_DNA.
DR EMBL; AK070720; BAG92109.1; -; mRNA.
DR EMBL; AK104435; BAG96680.1; -; mRNA.
DR RefSeq; XP_015627981.1; XM_015772495.1.
DR AlphaFoldDB; Q53RM0; -.
DR SMR; Q53RM0; -.
DR BioGRID; 802296; 1.
DR STRING; 4530.OS03T0563300-01; -.
DR PaxDb; Q53RM0; -.
DR PRIDE; Q53RM0; -.
DR EnsemblPlants; Os03t0563300-01; Os03t0563300-01; Os03g0563300.
DR GeneID; 4333259; -.
DR Gramene; Os03t0563300-01; Os03t0563300-01; Os03g0563300.
DR KEGG; osa:4333259; -.
DR eggNOG; ENOG502QRUY; Eukaryota.
DR HOGENOM; CLU_016684_0_0_1; -.
DR InParanoid; Q53RM0; -.
DR OMA; RISAVCG; -.
DR OrthoDB; 918663at2759; -.
DR UniPathway; UPA00668; -.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000007752; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR GO; GO:0009570; C:chloroplast stroma; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016851; F:magnesium chelatase activity; IEA:UniProtKB-EC.
DR GO; GO:0015995; P:chlorophyll biosynthetic process; IBA:GO_Central.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR045006; CHLI-like.
DR InterPro; IPR041628; ChlI/MoxR_AAA_lid.
DR InterPro; IPR011775; Mg_chelatase_ATPase-isu.
DR InterPro; IPR000523; Mg_chelatse_chII-like_cat_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR32039; PTHR32039; 1.
DR Pfam; PF17863; AAA_lid_2; 1.
DR Pfam; PF01078; Mg_chelatase; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR02030; BchI-ChlI; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Chlorophyll biosynthesis; Chloroplast; Disulfide bond; Ligase;
KW Nucleotide-binding; Photosynthesis; Plastid; Reference proteome;
KW Transit peptide.
FT TRANSIT 1..67
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 68..415
FT /note="Magnesium-chelatase subunit ChlI, chloroplastic"
FT /id="PRO_0000418771"
FT DISULFID 93..184
FT /evidence="ECO:0000250"
FT DISULFID 345..387
FT /note="Inhibitory under oxidizing conditions"
FT /evidence="ECO:0000250"
FT MUTAGEN 307
FT /note="R->C: In chl9; yellowish-green leaf phenotype."
FT /evidence="ECO:0000269|PubMed:16915519"
SQ SEQUENCE 415 AA; 44865 MW; DEAB42A21C5877EE CRC64;
MASAFSPATA APAASPALFS ASTSRPLSLT AAAAAVSARI PSRRGFRRGR FTVCNVAAPS
ATQQEAKAAG AKESQRPVYP FAAIVGQDEM KLCLLLNVID PKIGGVMIMG DRGTGKSTTV
RSLVDLLPDI RVVVGDPFNS DPDDPEVMGP EVRERVLEGE KLPVVTAKIT MVDLPLGATE
DRVCGTIDIE KALTDGVKAF EPGLLAKANR GILYVDEVNL LDDHLVDVLL DSAASGWNTV
EREGISISHP ARFILIGSGN PEEGELRPQL LDRFGMHAQV GTVRDAELRV KIVEERARFD
RDPKAFRESY LEEQDKLQQQ ISSARSNLGA VQIDHDLRVK ISKVCAELNV DGLRGDIVTN
RAAKALAALK GRDTVTVEDI ATVIPNCLRH RLRKDPLESI DSGLLVVEKF YEVFT
