ID   CHLI_TOBAC              Reviewed;         426 AA.
AC   O22436;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Magnesium-chelatase subunit ChlI, chloroplastic;
DE            Short=Mg-chelatase subunit I-1;
DE            EC=6.6.1.1;
DE   AltName: Full=Mg-protoporphyrin IX chelatase subunit ChlI;
DE   Flags: Precursor;
GN   Name=CHLI;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. SR1;
RX   PubMed=9426628; DOI=10.1023/a:1005913315905;
RA   Kruse E., Mock H.-P., Grimm B.;
RT   "Isolation and characterisation of tobacco (Nicotiana tabacum) cDNA clones
RT   encoding proteins involved in magnesium chelation into protoporphyrin IX.";
RL   Plant Mol. Biol. 35:1053-1056(1997).
CC   -!- FUNCTION: Involved in chlorophyll biosynthesis. Catalyzes the insertion
CC       of magnesium ion into protoporphyrin IX to yield Mg-protoporphyrin IX.
CC       The magnesium-chelatase is a complex of three subunits, CHLI, CHLD and
CC       CHLH. The reaction takes place in two steps, with an ATP-dependent
CC       activation followed by an ATP-dependent chelation step (By similarity).
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O + Mg(2+) + protoporphyrin IX = ADP + 3 H(+) + Mg-
CC         protoporphyrin IX + phosphate; Xref=Rhea:RHEA:13961,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:18420,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:57306,
CC         ChEBI:CHEBI:60492, ChEBI:CHEBI:456216; EC=6.6.1.1;
CC   -!- ACTIVITY REGULATION: Redox regulation; active in reducing conditions,
CC       inactive in oxidizing conditions. Thioredoxins f and m mediate the
CC       reversible reductive activation of oxidized CHLI (By similarity).
CC       {ECO:0000250}.
CC   -!- PATHWAY: Porphyrin-containing compound metabolism; chlorophyll
CC       biosynthesis.
CC   -!- SUBUNIT: The magnesium chelatase complex is a heterotrimer consisting
CC       of subunits CHLI, CHLD, AND CHLH.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Strongly expressed in young leaves and to a lesser
CC       extent in mature leaves.
CC   -!- SIMILARITY: Belongs to the Mg-chelatase subunits D/I family.
CC       {ECO:0000305}.
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DR   EMBL; AF014053; AAB97153.1; -; mRNA.
DR   PIR; T01790; T01790.
DR   AlphaFoldDB; O22436; -.
DR   SMR; O22436; -.
DR   STRING; 4097.O22436; -.
DR   BioCyc; MetaCyc:MON-11779; -.
DR   UniPathway; UPA00668; -.
DR   Proteomes; UP000084051; Unplaced.
DR   GO; GO:0009570; C:chloroplast stroma; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016851; F:magnesium chelatase activity; IEA:UniProtKB-EC.
DR   GO; GO:0015995; P:chlorophyll biosynthetic process; IBA:GO_Central.
DR   GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR045006; CHLI-like.
DR   InterPro; IPR041628; ChlI/MoxR_AAA_lid.
DR   InterPro; IPR011775; Mg_chelatase_ATPase-isu.
DR   InterPro; IPR000523; Mg_chelatse_chII-like_cat_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR32039; PTHR32039; 1.
DR   Pfam; PF17863; AAA_lid_2; 1.
DR   Pfam; PF01078; Mg_chelatase; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR02030; BchI-ChlI; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Chlorophyll biosynthesis; Chloroplast; Disulfide bond; Ligase;
KW   Nucleotide-binding; Photosynthesis; Plastid; Reference proteome;
KW   Transit peptide.
FT   TRANSIT         1..50
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           51..426
FT                   /note="Magnesium-chelatase subunit ChlI, chloroplastic"
FT                   /id="PRO_0000002804"
FT   BINDING         121..128
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   DISULFID        104..195
FT                   /evidence="ECO:0000250"
FT   DISULFID        356..398
FT                   /note="Inhibitory under oxidizing conditions"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   426 AA;  46627 MW;  42B7A6F04E1C3274 CRC64;
     MASLLGTSSS AAAAILASTP LSSRSCKPAV FSLFPSSGQS QGRKFYGGIR VPVKKGRSQF
     HVAISNVATE INLLKNRVRN LLEESQRPVY PFAAIVGQDE MKLCLLLNVI DPKIGGVMIM
     GDRGTGKSTT VRSLVDLLPE IKVISGDPFN SDPDDQEVMS AEVRDKLRSG QQLPISRTKI
     NMVDLPLGAT EDRVCGTIDI EKALTEGVKA FEPGLLAKAN RGILYVDEVN LLDDHLVDVL
     LDSAASGWNT VEREGISISH PARFILIGSG NPEEGELRPQ LLDRFGMHAQ VGTVRDAELR
     VKIVEERARF DKNPKEFRES YKAEQEKLQN QIDSARNALS AVTIDHDLRV KISKVCAELN
     VDGLRGDIVT NRAARALAAL KGRDKVTPED IATVIPNCLR HRLRKDPLES IDSGVLVVEK
     FYEVFA