CHLP_TOBAC
ID CHLP_TOBAC Reviewed; 464 AA.
AC Q9ZS34;
DT 13-OCT-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=Geranylgeranyl diphosphate reductase, chloroplastic;
DE EC=1.3.1.83;
DE AltName: Full=Geranylgeranyl reductase;
DE Flags: Precursor;
GN Name=CHLP;
OS Nicotiana tabacum (Common tobacco).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC Nicotiana.
OX NCBI_TaxID=4097;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=10398704; DOI=10.1104/pp.120.3.695;
RA Tanaka R., Oster U., Kruse E., Ruediger W., Grimm B.;
RT "Reduced activity of geranylgeranyl reductase leads to loss of chlorophyll
RT and tocopherol and to partially geranylgeranylated chlorophyll in
RT transgenic tobacco plants expressing antisense RNA for geranylgeranyl
RT reductase.";
RL Plant Physiol. 120:695-704(1999).
CC -!- FUNCTION: Catalyzes the reduction of geranylgeranyl diphosphate to
CC phytyl diphosphate, providing phytol for both tocopherol and
CC chlorophyll synthesis. {ECO:0000269|PubMed:10398704}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3 NADP(+) + phytyl diphosphate = geranylgeranyl diphosphate +
CC 3 H(+) + 3 NADPH; Xref=Rhea:RHEA:26229, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57533, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349,
CC ChEBI:CHEBI:75434; EC=1.3.1.83;
CC -!- PATHWAY: Porphyrin-containing compound metabolism; chlorophyll
CC biosynthesis.
CC -!- PATHWAY: Cofactor biosynthesis; tocopherol biosynthesis.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Constant levels during the development of leaves.
CC {ECO:0000269|PubMed:10398704}.
CC -!- SIMILARITY: Belongs to the geranylgeranyl reductase family. ChlP
CC subfamily. {ECO:0000305}.
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DR EMBL; AJ007789; CAA07683.1; -; mRNA.
DR RefSeq; NP_001312288.1; NM_001325359.1.
DR AlphaFoldDB; Q9ZS34; -.
DR SMR; Q9ZS34; -.
DR STRING; 4097.Q9ZS34; -.
DR PRIDE; Q9ZS34; -.
DR GeneID; 107783257; -.
DR KEGG; ag:CAA07683; -.
DR KEGG; nta:107783257; -.
DR OMA; GMCRREV; -.
DR BioCyc; MetaCyc:MON-14870; -.
DR BRENDA; 1.3.1.83; 3645.
DR UniPathway; UPA00160; -.
DR UniPathway; UPA00668; -.
DR Proteomes; UP000084051; Unplaced.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0071949; F:FAD binding; IEA:InterPro.
DR GO; GO:0102067; F:geranylgeranyl diphosphate reductase activity; IEA:UniProtKB-EC.
DR GO; GO:0045550; F:geranylgeranyl reductase activity; IBA:GO_Central.
DR GO; GO:0015995; P:chlorophyll biosynthetic process; IMP:UniProtKB.
DR GO; GO:0033385; P:geranylgeranyl diphosphate metabolic process; IMP:UniProtKB.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR GO; GO:0033521; P:phytyl diphosphate biosynthetic process; IMP:UniProtKB.
DR GO; GO:0010189; P:vitamin E biosynthetic process; IMP:UniProtKB.
DR Gene3D; 3.50.50.60; -; 1.
DR InterPro; IPR010253; BchP_ChlP_pln/prok.
DR InterPro; IPR002938; FAD-bd.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR011777; Geranylgeranyl_Rdtase_fam.
DR InterPro; IPR011774; Geranylgeranyl_Rdtase_pln/cyn.
DR Pfam; PF01494; FAD_binding_3; 1.
DR SUPFAM; SSF51905; SSF51905; 1.
DR TIGRFAMs; TIGR02023; BchP-ChlP; 1.
DR TIGRFAMs; TIGR02028; ChlP; 1.
DR TIGRFAMs; TIGR02032; GG-red-SF; 1.
PE 2: Evidence at transcript level;
KW Chlorophyll biosynthesis; Chloroplast; NADP; Oxidoreductase;
KW Photosynthesis; Plastid; Reference proteome; Transit peptide.
FT TRANSIT 1..54
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 55..464
FT /note="Geranylgeranyl diphosphate reductase, chloroplastic"
FT /id="PRO_0000386544"
SQ SEQUENCE 464 AA; 51301 MW; 8F99F85EDDBFB765 CRC64;
MASIALKTFT GLRQSSPENN SITLSKSLPF TQTHRRLRIN ASKSSPRVNG RNLRVAVVGG
GPAGGAAAET LAKGGIETFL IERKMDNCKP CGGAIPLCMV GEFDLPLDII DRKVTKMKMI
SPSNVAVDIG QTLKPHEYIG MVRREVLDAY LRDRAAEAGA SVLNGLFLKM DMPKAPNAPY
VLHYTAYDSK TNGAGEKRTL EVDAVIGADG ANSRVAKSIN AGDYEYAIAF QERIKISDDK
MKYYENLAEM YVGDDVSPDF YGWVFPKCDH VAVGTGTVTH KADIKKFQLA TRLRADSKIT
GGKIIRVEAH PIPEHPRPRR LQDRVALVGD AAGYVTKCSG EGIYFAAKSG RMCAEAIVEG
SEMGKRMVDE SDLRKYLEKW DKTYWPTYKV LDILQKVFYR SNPAREAFVE MCADEYVQKM
TFDSYLYKKV APGNPIEDLK LAVNTIGSLV RANALRREMD KLSV