ID   CHM2B_PONAB             Reviewed;         213 AA.
AC   Q5RAV2;
DT   30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   21-DEC-2004, sequence version 1.
DT   03-AUG-2022, entry version 63.
DE   RecName: Full=Charged multivesicular body protein 2b;
DE   AltName: Full=Chromatin-modifying protein 2b;
DE            Short=CHMP2b;
GN   Name=CHMP2B;
OS   Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Pongo.
OX   NCBI_TaxID=9601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Heart;
RG   The German cDNA consortium;
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Probable core component of the endosomal sorting required for
CC       transport complex III (ESCRT-III) which is involved in multivesicular
CC       bodies (MVBs) formation and sorting of endosomal cargo proteins into
CC       MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by
CC       invagination and scission from the limiting membrane of the endosome
CC       and mostly are delivered to lysosomes enabling degradation of membrane
CC       proteins, such as stimulated growth factor receptors, lysosomal enzymes
CC       and lipids. The MVB pathway appears to require the sequential function
CC       of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly
CC       dissociate from the invaginating membrane before the ILV is released.
CC       The ESCRT machinery also functions in topologically equivalent membrane
CC       fission events, such as the terminal stages of cytokinesis and the
CC       budding of enveloped viruses (lentiviruses). ESCRT-III proteins are
CC       believed to mediate the necessary vesicle extrusion and/or membrane
CC       fission activities, possibly in conjunction with the AAA ATPase VPS4
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Probable core component of the endosomal sorting required for
CC       transport complex III (ESCRT-III). ESCRT-III components are thought to
CC       multimerize to form a flat lattice on the perimeter membrane of the
CC       endosome. Several assembly forms of ESCRT-III may exist that interact
CC       and act sequentially. Interacts with CHMP2A. Interacts with VPS4A.
CC       Interacts with VPS4B; the interaction is direct (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000250}. Late endosome
CC       membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the SNF7 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CR858910; CAH91108.1; -; mRNA.
DR   RefSeq; NP_001125647.1; NM_001132175.1.
DR   AlphaFoldDB; Q5RAV2; -.
DR   SMR; Q5RAV2; -.
DR   GeneID; 100172566; -.
DR   KEGG; pon:100172566; -.
DR   CTD; 25978; -.
DR   eggNOG; KOG3231; Eukaryota.
DR   InParanoid; Q5RAV2; -.
DR   OrthoDB; 1480977at2759; -.
DR   Proteomes; UP000001595; Unplaced.
DR   GO; GO:1904930; C:amphisome membrane; IEA:UniProt.
DR   GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR   GO; GO:0000776; C:kinetochore; IEA:UniProt.
DR   GO; GO:0005828; C:kinetochore microtubule; IEA:UniProt.
DR   GO; GO:0005765; C:lysosomal membrane; IEA:UniProt.
DR   GO; GO:0030496; C:midbody; IEA:UniProt.
DR   GO; GO:0032585; C:multivesicular body membrane; IEA:UniProt.
DR   GO; GO:0005643; C:nuclear pore; IEA:UniProt.
DR   GO; GO:0097352; P:autophagosome maturation; IEA:UniProt.
DR   GO; GO:1902774; P:late endosome to lysosome transport; IEA:UniProt.
DR   GO; GO:0061952; P:midbody abscission; IEA:UniProt.
DR   GO; GO:0007080; P:mitotic metaphase plate congression; IEA:UniProt.
DR   GO; GO:0071985; P:multivesicular body sorting pathway; IEA:UniProt.
DR   GO; GO:0060548; P:negative regulation of cell death; IEA:UniProt.
DR   GO; GO:0031468; P:nuclear membrane reassembly; IEA:UniProt.
DR   GO; GO:0001778; P:plasma membrane repair; IEA:UniProt.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   GO; GO:1901673; P:regulation of mitotic spindle assembly; IEA:UniProt.
DR   GO; GO:0043162; P:ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; IEA:UniProt.
DR   GO; GO:0046761; P:viral budding from plasma membrane; IEA:UniProt.
DR   GO; GO:0039702; P:viral budding via host ESCRT complex; IEA:UniProt.
DR   InterPro; IPR005024; Snf7_fam.
DR   PANTHER; PTHR10476; PTHR10476; 1.
DR   Pfam; PF03357; Snf7; 1.
PE   2: Evidence at transcript level;
KW   Acetylation; Coiled coil; Cytoplasm; Endosome; Membrane; Phosphoprotein;
KW   Protein transport; Reference proteome; Transport.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250|UniProtKB:Q9UQN3"
FT   CHAIN           2..213
FT                   /note="Charged multivesicular body protein 2b"
FT                   /id="PRO_0000211471"
FT   REGION          179..199
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          25..55
FT                   /evidence="ECO:0000255"
FT   MOTIF           201..211
FT                   /note="MIT-interacting motif"
FT   MOD_RES         2
FT                   /note="N-acetylalanine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9UQN3"
FT   MOD_RES         199
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9UQN3"
SQ   SEQUENCE   213 AA;  23907 MW;  BA192A0EAC45C19B CRC64;
     MASLFKKKTV DDVIKEQNRE LRGTQRAIIR DRAALEKQEK QLELEIKKMA KIGNKEACKV
     LAKQLVHLRK QKTRTFAVSS KVTSMSTQTK VMNSQMKMAG AMSTTAKTMQ AVNKKMDPQK
     TLQTMQNFQK ENMKMEMTEE MINDTLDDIF DGSDDEEESQ DIVNQVLDEI GIEISGKMAK
     APSAARSLPS ASTSKATISD EEIERQLKAL GVD