ID   CHMP4_DICDI             Reviewed;         215 AA.
AC   Q86H98; Q553K9;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=Charged multivesicular body protein 4;
DE   AltName: Full=Vacuolar protein-sorting-associated protein 32;
DE            Short=Dd-Vps32;
GN   Name=chmp4; Synonyms=snf7, vps32; ORFNames=DDB_G0275573;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=12097910; DOI=10.1038/nature00847;
RA   Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA   Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA   Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA   Noegel A.A.;
RT   "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL   Nature 418:79-85(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [3]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=15708561; DOI=10.1016/j.ydbio.2004.12.004;
RA   Mattei S., Ryves W.J., Blot B., Sadoul R., Harwood A.J., Satre M.,
RA   Klein G., Aubry L.;
RT   "Dd-Alix, a conserved endosome-associated protein, controls Dictyostelium
RT   development.";
RL   Dev. Biol. 279:99-113(2005).
CC   -!- FUNCTION: Probable core component of the endosomal sorting required for
CC       transport complex III (ESCRT-III) which is involved in multivesicular
CC       bodies (MVBs) formation and sorting of endosomal cargo proteins into
CC       MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by
CC       invagination and scission from the limiting membrane of the endosome
CC       and mostly are delivered to lysosomes enabling degradation of membrane
CC       proteins. {ECO:0000269|PubMed:15708561}.
CC   -!- SUBUNIT: Probable core component of the endosomal sorting required for
CC       transport complex III (ESCRT-III). ESCRT-III components are thought to
CC       multimerize to form a flat lattice on the perimeter membrane of the
CC       endosome (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane
CC       {ECO:0000269|PubMed:15708561}. Late endosome membrane {ECO:0000250};
CC       Peripheral membrane protein {ECO:0000250}. Note=Membrane-associated.
CC       Colocalizes to large vesicle-like structures with PDCD6IP/Alix.
CC   -!- SIMILARITY: Belongs to the SNF7 family. {ECO:0000305}.
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DR   EMBL; AAFI02000013; EAL69537.1; -; Genomic_DNA.
DR   RefSeq; XP_643404.1; XM_638312.1.
DR   AlphaFoldDB; Q86H98; -.
DR   SMR; Q86H98; -.
DR   STRING; 44689.DDB0231531; -.
DR   PaxDb; Q86H98; -.
DR   EnsemblProtists; EAL69537; EAL69537; DDB_G0275573.
DR   GeneID; 8619990; -.
DR   KEGG; ddi:DDB_G0275573; -.
DR   dictyBase; DDB_G0275573; vps32.
DR   eggNOG; KOG1656; Eukaryota.
DR   HOGENOM; CLU_071097_1_1_1; -.
DR   InParanoid; Q86H98; -.
DR   OMA; WSWFGGA; -.
DR   PhylomeDB; Q86H98; -.
DR   Reactome; R-DDI-1632852; Macroautophagy.
DR   Reactome; R-DDI-917729; Endosomal Sorting Complex Required For Transport (ESCRT).
DR   Reactome; R-DDI-9668328; Sealing of the nuclear envelope (NE) by ESCRT-III.
DR   PRO; PR:Q86H98; -.
DR   Proteomes; UP000002195; Chromosome 2.
DR   GO; GO:0009898; C:cytoplasmic side of plasma membrane; IBA:GO_Central.
DR   GO; GO:0005768; C:endosome; IDA:dictyBase.
DR   GO; GO:0000815; C:ESCRT III complex; ISS:dictyBase.
DR   GO; GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005771; C:multivesicular body; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR   GO; GO:0140220; C:pathogen-containing vacuole; IDA:dictyBase.
DR   GO; GO:0032010; C:phagolysosome; IDA:dictyBase.
DR   GO; GO:0008333; P:endosome to lysosome transport; IMP:UniProtKB.
DR   GO; GO:0045324; P:late endosome to vacuole transport; ISS:dictyBase.
DR   GO; GO:0032511; P:late endosome to vacuole transport via multivesicular body sorting pathway; IBA:GO_Central.
DR   GO; GO:0007040; P:lysosome organization; IMP:UniProtKB.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   GO; GO:0001919; P:regulation of receptor recycling; IMP:UniProtKB.
DR   GO; GO:0006900; P:vesicle budding from membrane; IBA:GO_Central.
DR   InterPro; IPR005024; Snf7_fam.
DR   PANTHER; PTHR22761; PTHR22761; 1.
DR   Pfam; PF03357; Snf7; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Cytoplasmic vesicle; Endosome; Membrane; Protein transport;
KW   Reference proteome; Transport.
FT   CHAIN           1..215
FT                   /note="Charged multivesicular body protein 4"
FT                   /id="PRO_0000328395"
FT   COILED          10..173
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   215 AA;  25045 MW;  CE32BD29747FB9CA CRC64;
     MKLFGKPKPK QDTQETIGKL RLTLDMLEKR QTFLQDKLEK EQEKAKVLVS QKRKREALLC
     LKKRNNFQNE VTKLQGSYDT LNQQIFALEN AKMNMEIMNS MRDGARSLRE LHGHLTIDKV
     DDVIEEIQEQ MEIHEEISNA ISQPLGNQVE DEEELLRELA EYEQEELDAQ LLNIKAPSRE
     LPQEIQVNFP TISKTVPKLS KEEEEIRALE ESLAM