CHMP6_MOUSE
ID CHMP6_MOUSE Reviewed; 200 AA.
AC P0C0A3;
DT 30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Charged multivesicular body protein 6;
DE AltName: Full=Chromatin-modifying protein 6;
GN Name=Chmp6;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Mammary gland;
RG The MGC Project Team;
RL Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-119, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Probable core component of the endosomal sorting required for
CC transport complex III (ESCRT-III) which is involved in multivesicular
CC bodies (MVBs) formation and sorting of endosomal cargo proteins into
CC MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by
CC invagination and scission from the limiting membrane of the endosome
CC and mostly are delivered to lysosomes enabling degradation of membrane
CC proteins, such as stimulated growth factor receptors, lysosomal enzymes
CC and lipids. The MVB pathway appears to require the sequential function
CC of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly
CC dissociate from the invaginating membrane before the ILV is released.
CC The ESCRT machinery also functions in topologically equivalent membrane
CC fission events, such as the terminal stages of cytokinesis. ESCRT-III
CC proteins are believed to mediate the necessary vesicle extrusion and/or
CC membrane fission activities, possibly in conjunction with the AAA
CC ATPase VPS4. In the ESCRT-III complex, it probably serves as an
CC acceptor for the ESCRT-II complex on endosomal membranes (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Probable core component of the endosomal sorting required for
CC transport complex III (ESCRT-III). ESCRT-III components are thought to
CC multimerize to form a flat lattice on the perimeter membrane of the
CC endosome. Several assembly forms of ESCRT-III may exist that interact
CC and act sequentially. Interacts with VPS4A; the interaction is direct.
CC Interacts with VPS4B; the interaction is direct. Interacts with CHMP4A,
CC CHMP4B and CHMP4C. Interacts with SNF8, VPS25 and VPS36 (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Endomembrane system {ECO:0000250}. Endosome
CC membrane {ECO:0000250}; Lipid-anchor {ECO:0000250}. Late endosome
CC membrane {ECO:0000250}. Membrane {ECO:0000250|UniProtKB:Q96FZ7}; Lipid-
CC anchor {ECO:0000250|UniProtKB:Q96FZ7}. Note=Localizes to endosomal
CC membranes. {ECO:0000250}.
CC -!- DOMAIN: The acidic C-terminus and the basic N-termminus are thought to
CC render the protein in a closed, soluble and inactive conformation
CC through an autoinhibitory intramolecular interaction. The open and
CC active conformation, which enables membrane binding and
CC oligomerization, is achieved by interaction with other cellular binding
CC partners, probably including other ESCRT components (By similarity).
CC {ECO:0000250}.
CC -!- PTM: ISGylated in a CHMP5-dependent manner. Isgylation weakens its
CC interaction with VPS4A (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the SNF7 family. {ECO:0000305}.
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DR EMBL; BI157958; -; NOT_ANNOTATED_CDS; mRNA.
DR CCDS; CCDS36385.1; -.
DR RefSeq; NP_001078967.1; NM_001085498.2.
DR AlphaFoldDB; P0C0A3; -.
DR SMR; P0C0A3; -.
DR BioGRID; 228946; 18.
DR ComplexPortal; CPX-332; ESCRT-III complex, variant Chmp1b1.
DR ComplexPortal; CPX-333; ESCRT-III complex, variant Chmp1b2.
DR IntAct; P0C0A3; 18.
DR STRING; 10090.ENSMUSP00000026434; -.
DR iPTMnet; P0C0A3; -.
DR PhosphoSitePlus; P0C0A3; -.
DR EPD; P0C0A3; -.
DR MaxQB; P0C0A3; -.
DR PaxDb; P0C0A3; -.
DR PRIDE; P0C0A3; -.
DR ProteomicsDB; 281618; -.
DR Antibodypedia; 19789; 163 antibodies from 24 providers.
DR Ensembl; ENSMUST00000026434; ENSMUSP00000026434; ENSMUSG00000025371.
DR GeneID; 208092; -.
DR KEGG; mmu:208092; -.
DR UCSC; uc007mrd.2; mouse.
DR CTD; 79643; -.
DR MGI; MGI:3583942; Chmp6.
DR VEuPathDB; HostDB:ENSMUSG00000025371; -.
DR eggNOG; KOG2910; Eukaryota.
DR GeneTree; ENSGT00720000108863; -.
DR HOGENOM; CLU_086201_3_0_1; -.
DR InParanoid; P0C0A3; -.
DR OMA; GTIEFKL; -.
DR OrthoDB; 1287094at2759; -.
DR PhylomeDB; P0C0A3; -.
DR TreeFam; TF105929; -.
DR Reactome; R-MMU-1632852; Macroautophagy.
DR Reactome; R-MMU-5620971; Pyroptosis.
DR Reactome; R-MMU-917729; Endosomal Sorting Complex Required For Transport (ESCRT).
DR Reactome; R-MMU-9668328; Sealing of the nuclear envelope (NE) by ESCRT-III.
DR BioGRID-ORCS; 208092; 32 hits in 74 CRISPR screens.
DR PRO; PR:P0C0A3; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; P0C0A3; protein.
DR Bgee; ENSMUSG00000025371; Expressed in right kidney and 219 other tissues.
DR ExpressionAtlas; P0C0A3; baseline and differential.
DR Genevisible; P0C0A3; MM.
DR GO; GO:1904930; C:amphisome membrane; ISO:MGI.
DR GO; GO:0000421; C:autophagosome membrane; ISO:MGI.
DR GO; GO:0010008; C:endosome membrane; ISO:MGI.
DR GO; GO:0000815; C:ESCRT III complex; ISO:MGI.
DR GO; GO:0000776; C:kinetochore; ISO:MGI.
DR GO; GO:0005828; C:kinetochore microtubule; ISO:MGI.
DR GO; GO:0005765; C:lysosomal membrane; ISO:MGI.
DR GO; GO:0030496; C:midbody; ISO:MGI.
DR GO; GO:0005771; C:multivesicular body; IBA:GO_Central.
DR GO; GO:0032585; C:multivesicular body membrane; ISO:MGI.
DR GO; GO:0005643; C:nuclear pore; ISO:MGI.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0047485; F:protein N-terminus binding; ISO:MGI.
DR GO; GO:0044877; F:protein-containing complex binding; ISO:MGI.
DR GO; GO:0097352; P:autophagosome maturation; ISO:MGI.
DR GO; GO:0006914; P:autophagy; ISO:MGI.
DR GO; GO:1902774; P:late endosome to lysosome transport; ISO:MGI.
DR GO; GO:0032511; P:late endosome to vacuole transport via multivesicular body sorting pathway; IBA:GO_Central.
DR GO; GO:0090148; P:membrane fission; IC:ComplexPortal.
DR GO; GO:0061952; P:midbody abscission; ISO:MGI.
DR GO; GO:0007080; P:mitotic metaphase plate congression; ISO:MGI.
DR GO; GO:0036258; P:multivesicular body assembly; IC:ComplexPortal.
DR GO; GO:0071985; P:multivesicular body sorting pathway; ISO:MGI.
DR GO; GO:0061763; P:multivesicular body-lysosome fusion; IC:ComplexPortal.
DR GO; GO:0060548; P:negative regulation of cell death; ISO:MGI.
DR GO; GO:0007175; P:negative regulation of epidermal growth factor-activated receptor activity; ISO:MGI.
DR GO; GO:0031468; P:nuclear membrane reassembly; ISO:MGI.
DR GO; GO:0006997; P:nucleus organization; ISO:MGI.
DR GO; GO:0001778; P:plasma membrane repair; ISO:MGI.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:1901673; P:regulation of mitotic spindle assembly; ISO:MGI.
DR GO; GO:0042176; P:regulation of protein catabolic process; ISO:MGI.
DR GO; GO:0043162; P:ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; ISO:MGI.
DR GO; GO:0006900; P:vesicle budding from membrane; IBA:GO_Central.
DR GO; GO:0051469; P:vesicle fusion with vacuole; IC:ComplexPortal.
DR GO; GO:0046761; P:viral budding from plasma membrane; ISO:MGI.
DR GO; GO:0039702; P:viral budding via host ESCRT complex; ISO:MGI.
DR InterPro; IPR005024; Snf7_fam.
DR PANTHER; PTHR22761; PTHR22761; 1.
DR Pfam; PF03357; Snf7; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Endosome; Lipoprotein; Membrane; Myristate; Phosphoprotein;
KW Protein transport; Reference proteome; Transport; Ubl conjugation.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q96FZ7"
FT CHAIN 2..200
FT /note="Charged multivesicular body protein 6"
FT /id="PRO_0000211509"
FT REGION 168..200
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 10..145
FT /evidence="ECO:0000255"
FT MOTIF 168..179
FT /note="Type-2 MIT-interacting motif"
FT /evidence="ECO:0000250"
FT MOD_RES 119
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 130
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q96FZ7"
FT LIPID 2
FT /note="N-myristoyl glycine"
FT /evidence="ECO:0000250|UniProtKB:Q96FZ7"
SQ SEQUENCE 200 AA; 23416 MW; 13EE6683997D46C6 CRC64;
MGNLFGRKKQ SRVTEQDRAI LQLKQQRDKL RQYQKRVTQQ LERERALARQ LLRDGRKERA
KLLLKKKRYR EQLLDRTENQ ISSLEAMVQS IEFTQIEMKV MEGLQVGNEC LNKMHQVMSI
EEVERILDET QEAVEYQRQI DELLAGNFTQ EDEDAILEEL NAITQEQMEL PEVPSEPLPD
RNPEAPAKAR SRQAELVAAS
