CHNA_RHOS6
ID CHNA_RHOS6 Reviewed; 270 AA.
AC Q6TMA3;
DT 29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 68.
DE RecName: Full=Cyclohexanol dehydrogenase {ECO:0000303|Ref.2};
DE EC=1.1.1.245 {ECO:0000269|Ref.1, ECO:0000269|Ref.2};
DE AltName: Full=Cyclohexanol dehydrogenase II {ECO:0000303|Ref.2};
DE Short=CHD II {ECO:0000303|Ref.2};
GN Name=chnA {ECO:0000303|Ref.1};
OS Rhodococcus sp. (strain TK6).
OC Bacteria; Actinobacteria; Corynebacteriales; Nocardiaceae; Rhodococcus.
OX NCBI_TaxID=249095;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, AND
RP BIOPHYSICOCHEMICAL PROPERTIES.
RA Choi J.-H., Kim T.-K., Kim Y.-M., Kim W.-C., Joo G.-J., Lee K.Y.,
RA Rhee I.-K.;
RT "Cloning and characterization of cyclohexanol dehydrogenase gene from
RT Rhodococcus sp. TK6.";
RL J. Microbiol. Biotechnol. 15:1189-1196(2005).
RN [2]
RP PROTEIN SEQUENCE OF 10-24, FUNCTION, CATALYTIC ACTIVITY, ACTIVITY
RP REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION,
RP AND INDUCTION.
RA Kim T.-K., Choi J.-H., Rhee I.-K.;
RT "Purification and characterization of a cyclohexanol dehydrogenase from
RT Rhodococcus sp. TK6.";
RL J. Microbiol. Biotechnol. 12:39-45(2002).
CC -!- FUNCTION: Catalyzes the oxidation of cyclohexanol to cyclohexanone. Can
CC also use a broad range of other alcohols, including trans-cyclohexane-
CC 1,2-diol, trans-cyclopentane-1,2-diol, cyclopentanol, hexane-1,2-diol,
CC ethanol, 1-propanol, 1-butanol, 1-pentanol and 1-hexanol.
CC {ECO:0000269|Ref.1, ECO:0000269|Ref.2}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=cyclohexanol + NAD(+) = cyclohexanone + H(+) + NADH;
CC Xref=Rhea:RHEA:10044, ChEBI:CHEBI:15378, ChEBI:CHEBI:17854,
CC ChEBI:CHEBI:18099, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945;
CC EC=1.1.1.245; Evidence={ECO:0000269|Ref.1, ECO:0000269|Ref.2};
CC -!- ACTIVITY REGULATION: Activity is enhanced by the addition of Ba(2+) and
CC Mg(2+), but inhibited by the addition of Al(3+), Ca(2+), Co(2+),
CC Cu(2+), Mn(2+) and Zn(2+). {ECO:0000269|Ref.2}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=1.7 mM for cyclohexanol {ECO:0000269|Ref.2};
CC KM=2.8 mM for trans-cyclohexane-1,2-diol {ECO:0000269|Ref.2};
CC KM=14.2 mM for cyclopentanol {ECO:0000269|Ref.2};
CC KM=13.7 mM for trans-cyclopentane-1,2-diol {ECO:0000269|Ref.2};
CC KM=13.5 mM for hexane-1,2-diol {ECO:0000269|Ref.2};
CC pH dependence:
CC Optimum pH is 8.0 (Ref.1). Stable between pH 7.5 and 8.5 (Ref.2).
CC {ECO:0000269|Ref.1, ECO:0000269|Ref.2};
CC Temperature dependence:
CC Rapidly inactivated at temperatures above 40 degrees Celsius.
CC {ECO:0000269|Ref.2};
CC -!- SUBUNIT: Homodimer. {ECO:0000269|Ref.2}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305|Ref.2}.
CC -!- INDUCTION: Induced by cyclohexanol. {ECO:0000269|Ref.2}.
CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC family. {ECO:0000305}.
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DR EMBL; AY394000; AAR27575.1; -; Genomic_DNA.
DR SMR; Q6TMA3; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR002347; SDR_fam.
DR PRINTS; PR00081; GDHRDH.
DR PRINTS; PR00080; SDRFAMILY.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Direct protein sequencing; NAD; Oxidoreductase.
FT CHAIN 1..270
FT /note="Cyclohexanol dehydrogenase"
FT /id="PRO_0000453523"
FT ACT_SITE 176
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:P9WGT1"
FT BINDING 19..21
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250|UniProtKB:P9WGT1"
FT BINDING 40
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250|UniProtKB:P9WGT1"
FT BINDING 78..79
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250|UniProtKB:P9WGT1"
FT BINDING 105
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250|UniProtKB:P9WGT1"
FT BINDING 176
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250|UniProtKB:P9WGT1"
FT BINDING 180
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250|UniProtKB:P9WGT1"
FT CONFLICT 10
FT /note="K -> T (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 13
FT /note="L -> H (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 270 AA; 28376 MW; 862540D116FC718B CRC64;
MTDNLPLRGK VALVTGAARG IGRAYALRLA KRGADVAVVD FDLHSYKDYQ LEAASMRGDT
VVDEIREIGM RALGFQADVT DATTLNEAVQ QIVGEWGRLD IAICNAGGGV GSPEETRASI
VEKDLVDVVV ARNLTGTIHT CQAVAVPMKE QRSGKIVTVG SQAGHRIEDN GGYAHYGAAK
AAVAKYTQYL ARDLGPFGVT VNCVAPGYIS TGRLAPILSA MGDAQLLDDV PLGRYGTPED
CAGVIEFLSS DLSDYVTGAI IPVDGGLTYS
