CHNMO_DROME
ID CHNMO_DROME Reviewed; 604 AA.
AC Q9VQ30; Q8IGM8; Q9VQ31;
DT 30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 3.
DT 03-AUG-2022, entry version 183.
DE RecName: Full=Zinc finger protein chinmo {ECO:0000305};
DE AltName: Full=Protein chronologically inappropriate morphogenesis {ECO:0000303|PubMed:17055440};
GN Name=chinmo {ECO:0000303|PubMed:17055440, ECO:0000312|FlyBase:FBgn0086758};
GN ORFNames=CG31666 {ECO:0000312|FlyBase:FBgn0086758};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAL25503.1, ECO:0000312|EMBL:AAN71452.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAL25503.1, ECO:0000312|EMBL:AAN71452.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:AAL25503.1, ECO:0000312|EMBL:AAN71452.1};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000312|EMBL:ACI88731.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:ACI88731.1};
RA Carlson J., Booth B., Frise E., Park S., Wan K., Yu C., Celniker S.;
RL Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP DISRUPTION PHENOTYPE, AND MUTAGENESIS OF PHE-88.
RX PubMed=17055440; DOI=10.1016/j.cell.2006.08.045;
RA Zhu S., Lin S., Kao C.F., Awasaki T., Chiang A.S., Lee T.;
RT "Gradients of the Drosophila Chinmo BTB-zinc finger protein govern neuronal
RT temporal identity.";
RL Cell 127:409-422(2006).
RN [6] {ECO:0000305}
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=20412771; DOI=10.1016/j.devcel.2010.02.006;
RA Flaherty M.S., Salis P., Evans C.J., Ekas L.A., Marouf A., Zavadil J.,
RA Banerjee U., Bach E.A.;
RT "chinmo is a functional effector of the JAK/STAT pathway that regulates eye
RT development, tumor formation, and stem cell self-renewal in Drosophila.";
RL Dev. Cell 18:556-568(2010).
RN [7] {ECO:0000305}
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=25453558; DOI=10.1016/j.devcel.2014.10.004;
RA Ma Q., Wawersik M., Matunis E.L.;
RT "The Jak-STAT target Chinmo prevents sex transformation of adult stem cells
RT in the Drosophila testis niche.";
RL Dev. Cell 31:474-486(2014).
RN [8] {ECO:0000305}
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=26021350; DOI=10.1242/jcs.169441;
RA Dopie J., Rajakylae E.K., Joensuu M.S., Huet G., Ferrantelli E., Xie T.,
RA Jaeaelinoja H., Jokitalo E., Vartiainen M.K.;
RT "Genome-wide RNAi screen for nuclear actin reveals a network of cofilin
RT regulators.";
RL J. Cell Sci. 128:2388-2400(2015).
RN [9] {ECO:0000305}
RP DISRUPTION PHENOTYPE.
RX PubMed=26811385; DOI=10.1242/dev.129627;
RA Ma Q., de Cuevas M., Matunis E.L.;
RT "Chinmo is sufficient to induce male fate in somatic cells of the adult
RT Drosophila ovary.";
RL Development 143:754-763(2016).
RN [10]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=29389999; DOI=10.1371/journal.pgen.1007203;
RA Grmai L., Hudry B., Miguel-Aliaga I., Bach E.A.;
RT "Chinmo prevents transformer alternative splicing to maintain male sex
RT identity.";
RL PLoS Genet. 14:e1007203-e1007203(2018).
CC -!- FUNCTION: Required for morphological differentiation of postmitotic
CC neurons during postembryonic brain development (PubMed:17055440).
CC Ensures production of appropriate neuron subtypes within a lineage by
CC preventing precocious generation of late neuronal types of that lineage
CC (PubMed:17055440). Acts as a downstream mediator of the transcriptional
CC activator Stat92e and is required for the development of the eye-
CC antennal disk which gives rise to the adult eye, antenna and head
CC capsule, for transcriptional repression of the Notch receptor ligand
CC Ser and for the self-renewal of cyst stem cells in the testis
CC (PubMed:20412771). In the adult testis, maintains the male identify of
CC adult somatic cyst stem cells (PubMed:25453558, PubMed:29389999).
CC Represses expression and alternative splicing of transformer pre-mRNA,
CC resulting in the production of the male-specific isoform of
CC transcription factor dsx which ensures male-specific transcription of
CC target genes (PubMed:25453558, PubMed:29389999). Plays a role in actin
CC nuclear localization through its involvement in repressing the
CC expression of the kinase Cdi (PubMed:26021350). This maintains the
CC cofilin/actin-depolymerizing factor homolog tsr in its unphosphorylated
CC state which is required for actin nuclear import (PubMed:26021350).
CC {ECO:0000269|PubMed:17055440, ECO:0000269|PubMed:20412771,
CC ECO:0000269|PubMed:25453558, ECO:0000269|PubMed:26021350,
CC ECO:0000269|PubMed:29389999}.
CC -!- INTERACTION:
CC Q9VQ30; M9PFG6: Sox21a; NbExp=2; IntAct=EBI-93669, EBI-22059119;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:17055440}.
CC -!- TISSUE SPECIFICITY: Broadly expressed in the developing larval central
CC nervous system (at protein level) (PubMed:17055440). Expressed in the
CC larval lymph gland and circulating hemocytes (at protein level)
CC (PubMed:20412771). Expressed in all cell types of the adult testis stem
CC cell niche but not detected in somatic cells of the adult ovary (at
CC protein level) (PubMed:29389999). In the testis, expressed at high
CC levels in cyst stem cells and early cyst cells and, at lower levels, in
CC germline stem cells (at protein level) (PubMed:20412771).
CC {ECO:0000269|PubMed:17055440, ECO:0000269|PubMed:20412771,
CC ECO:0000269|PubMed:29389999}.
CC -!- DEVELOPMENTAL STAGE: In the mushroom body, abundant at early larval
CC stages, becomes much reduced in wandering larvae and is undetectable in
CC young pupae. Expressed at higher levels in postmitotic neurons born at
CC early postembryonic developmental stages than in later-born neurons of
CC the same lineage. {ECO:0000269|PubMed:17055440}.
CC -!- DISRUPTION PHENOTYPE: Inappropriate morphogenesis of larval mushroom
CC body neurons with early-born neurons adopting the fate of late-born
CC neurons from the same lineage (PubMed:17055440). Malformed eyes and
CC head capsules due to defects in eye progenitor cells (PubMed:20412771).
CC RNAi-mediated knockdown in adult cyst stem cells and early cyst cells
CC results in the appearance of cells resembling ovarian follicle cells
CC throughout the testis (PubMed:25453558). RNAi-mediated knockdown in
CC adult cyst stem cells results in loss of expression of the male-
CC specific isoform of transcription factor dsx and leads to male
CC sterility (PubMed:29389999). RNAi-mediated knockdown in imaginal wing
CC disks results in decreased levels of nuclear actin (PubMed:26021350).
CC RNAi-mediated knockdown in adult ovary somatic stem cells results in
CC ovaries which are morphologically indistinguishable from the wild-type
CC while ectopic expression in the adult ovary is sufficient to induce
CC male fate in somatic cells (PubMed:26811385).
CC {ECO:0000269|PubMed:17055440, ECO:0000269|PubMed:20412771,
CC ECO:0000269|PubMed:25453558, ECO:0000269|PubMed:26021350,
CC ECO:0000269|PubMed:26811385, ECO:0000269|PubMed:29389999}.
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DR EMBL; AE014134; AAF51350.2; -; Genomic_DNA.
DR EMBL; AE014134; AAF51352.3; -; Genomic_DNA.
DR EMBL; AE014134; AAN10454.1; -; Genomic_DNA.
DR EMBL; AE014134; AFH03516.1; -; Genomic_DNA.
DR EMBL; AY060464; AAL25503.1; -; mRNA.
DR EMBL; BT001697; AAN71452.1; -; mRNA.
DR EMBL; BT050405; ACI88731.1; -; mRNA.
DR RefSeq; NP_001245839.1; NM_001258910.2.
DR RefSeq; NP_608610.1; NM_134766.6.
DR RefSeq; NP_722718.1; NM_164429.3.
DR RefSeq; NP_722719.1; NM_164430.4.
DR AlphaFoldDB; Q9VQ30; -.
DR SMR; Q9VQ30; -.
DR IntAct; Q9VQ30; 14.
DR STRING; 7227.FBpp0297890; -.
DR DNASU; 33343; -.
DR EnsemblMetazoa; FBtr0077877; FBpp0077545; FBgn0086758.
DR EnsemblMetazoa; FBtr0077878; FBpp0077546; FBgn0086758.
DR EnsemblMetazoa; FBtr0303935; FBpp0292934; FBgn0086758.
DR EnsemblMetazoa; FBtr0307047; FBpp0297890; FBgn0086758.
DR GeneID; 33343; -.
DR KEGG; dme:Dmel_CG31666; -.
DR UCSC; CG31666-RA; d. melanogaster.
DR CTD; 33343; -.
DR FlyBase; FBgn0086758; chinmo.
DR VEuPathDB; VectorBase:FBgn0086758; -.
DR eggNOG; ENOG502RRBM; Eukaryota.
DR HOGENOM; CLU_011721_3_0_1; -.
DR OMA; KNSSECP; -.
DR OrthoDB; 1229475at2759; -.
DR SignaLink; Q9VQ30; -.
DR BioGRID-ORCS; 33343; 1 hit in 1 CRISPR screen.
DR ChiTaRS; chinmo; fly.
DR GenomeRNAi; 33343; -.
DR PRO; PR:Q9VQ30; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0086758; Expressed in brain and 33 other tissues.
DR ExpressionAtlas; Q9VQ30; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0035214; P:eye-antennal disc development; IMP:UniProtKB.
DR GO; GO:0051170; P:import into nucleus; IMP:UniProtKB.
DR GO; GO:0019102; P:male somatic sex determination; IMP:UniProtKB.
DR GO; GO:0016319; P:mushroom body development; IMP:FlyBase.
DR GO; GO:0001933; P:negative regulation of protein phosphorylation; IMP:UniProtKB.
DR GO; GO:0048666; P:neuron development; IMP:UniProtKB.
DR GO; GO:0030838; P:positive regulation of actin filament polymerization; IMP:UniProtKB.
DR GO; GO:2000035; P:regulation of stem cell division; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0007548; P:sex differentiation; IEA:UniProtKB-KW.
DR Gene3D; 3.30.710.10; -; 1.
DR InterPro; IPR000210; BTB/POZ_dom.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00651; BTB; 1.
DR Pfam; PF00096; zf-C2H2; 1.
DR SMART; SM00225; BTB; 1.
DR SMART; SM00355; ZnF_C2H2; 2.
DR SUPFAM; SSF54695; SSF54695; 1.
DR SUPFAM; SSF57667; SSF57667; 1.
DR PROSITE; PS50097; BTB; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 2.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 2.
PE 1: Evidence at protein level;
KW Developmental protein; Differentiation; Metal-binding; Neurogenesis;
KW Nucleus; Reference proteome; Repeat; Sexual differentiation; Zinc;
KW Zinc-finger.
FT CHAIN 1..604
FT /note="Zinc finger protein chinmo"
FT /id="PRO_0000438593"
FT DOMAIN 32..98
FT /note="BTB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT ZN_FING 517..540
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 545..568
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 122..155
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 291..310
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 330..437
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 450..470
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 334..354
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 361..420
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 88
FT /note="F->I: In chinmo-M33; mushroom body neuroblast clones
FT contain increased numbers of late-born alpha/beta type
FT neurons and fewer early-born gamma type neurons."
FT /evidence="ECO:0000269|PubMed:17055440"
FT CONFLICT 497
FT /note="V -> A (in Ref. 3; AAN71452)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 604 AA; 62230 MW; 126AF0159DC41BF5 CRC64;
MDPQQQFCLK WNSFSSNLAI TFSNLFKSDL LADVILSCDG VVFKAHKLIL AACSKKFADL
FENTPTNGQC VIILEATTPD NMAALLEFMY KGEVHVSQEA LNSFLKSAES LQVKGLSTET
GRLAAQQAQQ HMGDLSPLDS PTGRRSVRNS LSGGSSSIVP GGVGIGLGGG ATGANSMSGM
GIGNGLSLAG MAAGGGMAAA ANAAASSLST LAASANIVDR CGSAGANIIS GSAAGIGGSH
SGGAGNGSGT VGIGGNGVGS GGGNNGPISL GSGAGAAHHL GGSTGILKQE CDSLMHPGGS
SSSSGMGYTH VPPIYRPINY EPPRKRAIVR SPYSEQEQRG SVLRDGSKSS ECPSPINKPP
YHRPSSSASS TAPTEADTMH SERASPQSSR YENHSPSTTA GNGNATSSLE RIVKSERNNG
SANEANDDDR ELMDESTDNG AEDLRVKLEN LKYSPPPPPN SNTSSTTPNT LLENLKADGT
LSSNLAASIA PADMLNVWNA TKMNNKNSVN TADGKKLKCL YCDRLYGYET NLRAHIRQRH
QGIRVPCPFC ERTFTRNNTV RRHIAREHKQ EIGLAAGATI APAHLAAAAA ASAAATAAAS
NHSP
