CHN_DROME
ID CHN_DROME Reviewed; 1214 AA.
AC Q7YU81; A4UZH6; Q0E968; Q5BHY4; Q8T065; Q95RE7; Q9V788;
DT 12-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 13-NOV-2007, sequence version 3.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Protein charlatan;
GN Name=chn; ORFNames=CG11798;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS A AND C).
RC STRAIN=Berkeley; TISSUE=Embryo, and Head;
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A.,
RA Gonzalez M., Guarin H., Kapadia B., Kronmiller B., Li P.W., Liao G.,
RA Miranda A., Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S.,
RA Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M.,
RA Celniker S.E.;
RL Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 135-1214 (ISOFORM A).
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=15703278; DOI=10.1242/dev.01691;
RA Escudero L.M., Caminero E., Schulze K.L., Bellen H.J., Modolell J.;
RT "Charlatan, a Zn-finger transcription factor, establishes a novel level of
RT regulation of the proneural achaete/scute genes of Drosophila.";
RL Development 132:1211-1222(2005).
CC -!- FUNCTION: Probable transcription factor involved in the development of
CC the adult pattern of macrochaetae. Required for accumulation of achaete
CC (ac) and scute (sc) in proneural clusters. Probably acts by binding to
CC the proneural cluster-specific enhancers of the ac/sc complex and
CC increasing enhancer efficiency, thereby acting as a stimulator of ac/sc
CC expression in proneural clusters. Also required for correct development
CC of the embryonic/larval peripheral nervous system (PNS).
CC {ECO:0000269|PubMed:15703278}.
CC -!- INTERACTION:
CC Q7YU81; Q94527: Rel; NbExp=3; IntAct=EBI-164494, EBI-869024;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=C;
CC IsoId=Q7YU81-1; Sequence=Displayed;
CC Name=A; Synonyms=B;
CC IsoId=Q7YU81-2; Sequence=VSP_029165;
CC Name=D;
CC IsoId=Q7YU81-3; Sequence=VSP_029166, VSP_029167;
CC -!- TISSUE SPECIFICITY: Expressed in the PNS and CNS. In early blastoderm
CC stages, it is ubiquitously expressed, then, before stage 5, it
CC disappears from the poles of the embryo and faint stripes are visible.
CC At stage 5, it also accumulates in the dorsal region, cephalic furrow
CC ectodermal patches between the tracheal pits, where neurons of the PNS
CC appear. In older embryos (stage 15) a strong expression is mostly
CC restricted to the central nervous system (CNS) and PNS. In PNS, the
CC pattern suggests that expression occur in many of the neurons of the
CC ventral, lateral and dorsal clusters of neurons. In third instar wing
CC disks, it is expressed in rows of cells on either side of the
CC prospective anterior wing margin and in groups of cells that coincide
CC with proneural clusters of ac/sc expression. Also expressed
CC independently of ac/sc in certain areas of the disk, such as the
CC postnotum and posterior dorsal proximal wing. Expressed in the
CC proneural clusters of the leg disks and in the eye/antenna disk.
CC {ECO:0000269|PubMed:15703278}.
CC -!- INDUCTION: Up-regulated by ac/sc in proneural clusters of the wing
CC disk. {ECO:0000269|PubMed:15703278}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL28984.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAL39669.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AE013599; AAF58171.1; -; Genomic_DNA.
DR EMBL; AE013599; AAS64853.1; -; Genomic_DNA.
DR EMBL; AE013599; ABI31095.1; -; Genomic_DNA.
DR EMBL; AE013599; ABI31096.1; -; Genomic_DNA.
DR EMBL; BT009939; AAQ22408.1; -; mRNA.
DR EMBL; BT021440; AAX33588.1; -; mRNA.
DR EMBL; AY061436; AAL28984.1; ALT_INIT; mRNA.
DR EMBL; AY069524; AAL39669.1; ALT_INIT; mRNA.
DR RefSeq; NP_001036546.1; NM_001043081.2. [Q7YU81-3]
DR RefSeq; NP_001036547.1; NM_001043082.2. [Q7YU81-1]
DR RefSeq; NP_611013.1; NM_137169.4. [Q7YU81-2]
DR RefSeq; NP_995841.1; NM_206119.2. [Q7YU81-2]
DR AlphaFoldDB; Q7YU81; -.
DR BioGRID; 62418; 37.
DR IntAct; Q7YU81; 16.
DR STRING; 7227.FBpp0293744; -.
DR PaxDb; Q7YU81; -.
DR PRIDE; Q7YU81; -.
DR DNASU; 36678; -.
DR EnsemblMetazoa; FBtr0087415; FBpp0086546; FBgn0015371. [Q7YU81-2]
DR EnsemblMetazoa; FBtr0087416; FBpp0086547; FBgn0015371. [Q7YU81-2]
DR EnsemblMetazoa; FBtr0111006; FBpp0110305; FBgn0015371. [Q7YU81-1]
DR EnsemblMetazoa; FBtr0111007; FBpp0110306; FBgn0015371. [Q7YU81-3]
DR GeneID; 36678; -.
DR KEGG; dme:Dmel_CG11798; -.
DR CTD; 36678; -.
DR FlyBase; FBgn0015371; chn.
DR VEuPathDB; VectorBase:FBgn0015371; -.
DR eggNOG; KOG1721; Eukaryota.
DR InParanoid; Q7YU81; -.
DR OMA; HNAGNAF; -.
DR SignaLink; Q7YU81; -.
DR BioGRID-ORCS; 36678; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 36678; -.
DR PRO; PR:Q7YU81; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0015371; Expressed in brain and 79 other tissues.
DR ExpressionAtlas; Q7YU81; baseline and differential.
DR Genevisible; Q7YU81; DM.
DR GO; GO:0005634; C:nucleus; HDA:FlyBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IDA:UniProtKB.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; IDA:FlyBase.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:FlyBase.
DR GO; GO:0048813; P:dendrite morphogenesis; IMP:FlyBase.
DR GO; GO:0001700; P:embryonic development via the syncytial blastoderm; IMP:UniProtKB.
DR GO; GO:0001654; P:eye development; IMP:FlyBase.
DR GO; GO:0007526; P:larval somatic muscle development; IMP:FlyBase.
DR GO; GO:0042683; P:negative regulation of compound eye cone cell fate specification; IMP:FlyBase.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:FlyBase.
DR GO; GO:0007422; P:peripheral nervous system development; IMP:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:UniProtKB.
DR GO; GO:0007458; P:progression of morphogenetic furrow involved in compound eye morphogenesis; IMP:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0007423; P:sensory organ development; IMP:UniProtKB.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR SMART; SM00355; ZnF_C2H2; 6.
DR SUPFAM; SSF57667; SSF57667; 2.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 1.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 2.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; DNA-binding; Metal-binding; Nucleus;
KW Reference proteome; Repeat; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..1214
FT /note="Protein charlatan"
FT /id="PRO_0000046916"
FT ZN_FING 305..327
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 333..356
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 496..518
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 522..545
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 213..238
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 250..284
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 367..397
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 463..488
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 741..790
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 848..946
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1062..1084
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 213..227
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 250..281
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 463..481
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 741..756
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 766..790
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 859..901
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 907..921
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 572..677
FT /note="Missing (in isoform A)"
FT /evidence="ECO:0000303|PubMed:12537569, ECO:0000303|Ref.3"
FT /id="VSP_029165"
FT VAR_SEQ 572..640
FT /note="GNVGNANGGGGAVTIYTTTTNEGVAGGGGGGGGGISGNISGGGPLQEIIVNP
FT SSMVGWRLSANGSLIPP -> PHDNRPARSGNGNQSGGSSSGNSNGNGGGNGNNNANAG
FT GHSQNLNVLRTIGNSLVANNQLNTYAGNAFG (in isoform D)"
FT /evidence="ECO:0000305"
FT /id="VSP_029166"
FT VAR_SEQ 641..1214
FT /note="Missing (in isoform D)"
FT /evidence="ECO:0000305"
FT /id="VSP_029167"
FT CONFLICT 204
FT /note="S -> T (in Ref. 3; AAQ22408)"
FT /evidence="ECO:0000305"
FT CONFLICT 743
FT /note="S -> G (in Ref. 3; AAQ22408)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1214 AA; 130303 MW; 5B08638C0712CB13 CRC64;
MATLIPVNGG HPAASGQSSN VEATYEDMFK EITRKLYGEE TGNGLHTLGT PVAQVATSGP
TAVPEGEQRS FTNLQQLDRS AAPSIEYESS AAGASGNNVA TTQANVIQQQ QQQQQQAESG
NSVVVTASSG ATVVPAPSVA AVGGFKSEDH LSTAFGLAAL MQNGFAAGQA GLLKAGEQQQ
RWAQDGSGLV AAAAAEPQLV QWTSGGKLQS YAHVNQQQQQ QQQPHQSTPK SKKHRQEHAA
ELIYASPSTS ANAAQNLAQS TPTSAPSNSS GGSTSSSGGG GGRKKAAQAA AAAAAANGVH
IQKRYACTHC PYSTDRRDLY TRHENIHKDE KPFQCYACLK QFNRADHVKK HFLRMHRELQ
YDINKTRRHV SAGSGSSGSG SSGSGSHHSG GRGNVTINSA GVNIDNAFLE AQRHPTSSSM
SIVETIEAVA SATDMPLAQL KQEKMDDGAG VVLPLHVGVM QQPVASSSSG SSGSHGGNGN
GGSGSGLLKP KREKRFTCCY CPWSGADKWG LKRHLNTHTK PFVCLLCDYK AARSERLATH
VLKVHNKRAC SKCSYLADTQ EEYQAHMSDV HGNVGNANGG GGAVTIYTTT TNEGVAGGGG
GGGGGISGNI SGGGPLQEII VNPSSMVGWR LSANGSLIPP HDLLTGGLPN AATQKRGSER
LFQYLEAEGS DPEDYARLLK MDAISRNTAS VAQDFHKAGG VHELKIPANH QLLFNNKLPS
QWTTREAAAL LYSLSNMGGG SASSVSGSQR QKFGMRARQH STGEDDENTP SSASSSSFSG
DEFNMSATSP LKLSRHAIKL EKMDEMDAKD MGPTKAMMAT AFLEAANYEQ TAIELLASKR
KIKVENDNDE DQENQQHQPH QQHHSQQQQQ QRLQLIKSSP AYKLNNNNNN NSNNNNYYKD
KTSHRNAVHH HRQDDKENNK TKSPGTAAVS VAAAAATSPP SISGPSNQTP FLTQMEYQNL
NRIGTQFQNY VKDIINKYYA AETPLMLAAA AAALPTATTT GQQQQPELDI ENLSPSKRRR
LLSETEEYIE YLRNKEDITL TIAPKVQPPA PVTSLLKRQL DLSTPRRSPK KAAPAHSNSA
SNASRKSLNQ LATLLPLLAD AASQQEYLAA PLDFSKKSSS RKQAQPKKIR LTPEAVVTLL
RDKYLNRMVR QRLGCLKCNQ LRKNSSISFN YHTLGSLALH KYWRHGRLGS SSTRREKLQA
ALQKRISRGQ ADKC
