ID   CHO_ECO57               Reviewed;         295 AA.
AC   Q8XDZ7;
DT   16-APR-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   03-AUG-2022, entry version 104.
DE   RecName: Full=Excinuclease cho;
DE            EC=3.1.25.-;
DE   AltName: Full=Endonuclease cho;
DE   AltName: Full=UvrC homolog protein;
GN   Name=cho; OrderedLocusNames=Z2771, ECs2447;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Incises the DNA at the 3' side of a lesion during nucleotide
CC       excision repair. Incises the DNA farther away from the lesion than
CC       UvrC. Not able to incise the 5' site of a lesion. When a lesion remains
CC       because UvrC is not able to induce the 3' incision, Cho incises the
CC       DNA. Then UvrC makes the 5' incision. The combined action of Cho and
CC       UvrC broadens the substrate range of nucleotide excision repair (By
CC       similarity). {ECO:0000250}.
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DR   EMBL; AE005174; AAG56727.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB35870.1; -; Genomic_DNA.
DR   PIR; C85783; C85783.
DR   PIR; G90934; G90934.
DR   RefSeq; NP_310474.1; NC_002695.1.
DR   RefSeq; WP_000252388.1; NZ_SWKA01000004.1.
DR   AlphaFoldDB; Q8XDZ7; -.
DR   SMR; Q8XDZ7; -.
DR   STRING; 155864.EDL933_2700; -.
DR   EnsemblBacteria; AAG56727; AAG56727; Z2771.
DR   EnsemblBacteria; BAB35870; BAB35870; ECs_2447.
DR   GeneID; 912511; -.
DR   KEGG; ece:Z2771; -.
DR   KEGG; ecs:ECs_2447; -.
DR   PATRIC; fig|386585.9.peg.2561; -.
DR   eggNOG; COG0322; Bacteria.
DR   HOGENOM; CLU_054721_1_0_6; -.
DR   OMA; RVMSHFR; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0009432; P:SOS response; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.1440.10; -; 1.
DR   InterPro; IPR000305; GIY-YIG_endonuc.
DR   InterPro; IPR035901; GIY-YIG_endonuc_sf.
DR   SMART; SM00465; GIYc; 1.
DR   SUPFAM; SSF82771; SSF82771; 1.
DR   PROSITE; PS50164; GIY_YIG; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA excision; DNA repair; Excision nuclease; Hydrolase;
KW   Reference proteome; SOS response.
FT   CHAIN           1..295
FT                   /note="Excinuclease cho"
FT                   /id="PRO_0000138372"
FT   DOMAIN          33..108
FT                   /note="GIY-YIG"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00977"
SQ   SEQUENCE   295 AA;  33725 MW;  7CBD768E4EEF4321 CRC64;
     MVRRLTSPRL EFEAAAIYEY PEHLRSFLND LPTRPGVYLF HGESDTMPLY IGKSVNIRSR
     VLSHLRTPDE AAMLRQSRRI SWICTAGEIG ALLLEARLIK EQQPLFNKRL RRNRQLCALQ
     LNEKRVDVVY AKEVDFSRAP NLFGLFANRR AALQALQSIA DEQKLCYGLL GLEPLSRGRA
     CFRSALKRCA GACCGKESHE EHALRLRQSL ERLRVVCWPW QGAVALKEQH PEMTQYHIIQ
     NWLWLGAVNS LEEATTLIRT PAGFDHDGYK ILCKPLLSGN YEITELDPAN DQRAS