ID   CHO_ECOL6               Reviewed;         295 AA.
AC   P59439;
DT   28-MAR-2003, integrated into UniProtKB/Swiss-Prot.
DT   28-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=Excinuclease cho;
DE            EC=3.1.25.-;
DE   AltName: Full=Endonuclease cho;
DE   AltName: Full=UvrC homolog protein;
GN   Name=cho; OrderedLocusNames=c2140;
OS   Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=199310;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=12471157; DOI=10.1073/pnas.252529799;
RA   Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D.,
RA   Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F.,
RA   Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T.,
RA   Donnenberg M.S., Blattner F.R.;
RT   "Extensive mosaic structure revealed by the complete genome sequence of
RT   uropathogenic Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002).
CC   -!- FUNCTION: Incises the DNA at the 3' side of a lesion during nucleotide
CC       excision repair. Incises the DNA farther away from the lesion than
CC       UvrC. Not able to incise the 5' site of a lesion. When a lesion remains
CC       because UvrC is not able to induce the 3' incision, Cho incises the
CC       DNA. Then UvrC makes the 5' incision. The combined action of Cho and
CC       UvrC broadens the substrate range of nucleotide excision repair (By
CC       similarity). {ECO:0000250}.
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DR   EMBL; AE014075; AAN80599.1; -; Genomic_DNA.
DR   RefSeq; WP_000252363.1; NC_004431.1.
DR   AlphaFoldDB; P59439; -.
DR   SMR; P59439; -.
DR   STRING; 199310.c2140; -.
DR   EnsemblBacteria; AAN80599; AAN80599; c2140.
DR   KEGG; ecc:c2140; -.
DR   eggNOG; COG0322; Bacteria.
DR   HOGENOM; CLU_054721_1_0_6; -.
DR   OMA; RVMSHFR; -.
DR   BioCyc; ECOL199310:C2140-MON; -.
DR   Proteomes; UP000001410; Chromosome.
DR   GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0009432; P:SOS response; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.1440.10; -; 1.
DR   InterPro; IPR000305; GIY-YIG_endonuc.
DR   InterPro; IPR035901; GIY-YIG_endonuc_sf.
DR   SMART; SM00465; GIYc; 1.
DR   SUPFAM; SSF82771; SSF82771; 1.
DR   PROSITE; PS50164; GIY_YIG; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA excision; DNA repair; Excision nuclease; Hydrolase;
KW   SOS response.
FT   CHAIN           1..295
FT                   /note="Excinuclease cho"
FT                   /id="PRO_0000138371"
FT   DOMAIN          33..108
FT                   /note="GIY-YIG"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00977"
SQ   SEQUENCE   295 AA;  33694 MW;  9278739AB3E9130E CRC64;
     MVRRLTSPRL EFEAAAIYEY PEHLRSFLND LPTRPGVYLF HGESDTMPLY IGKSINIRSR
     VLSHLRTPDE AAMLRQSRRI SWICTAGEIG ALLLEARLIK EQQPLFNKRL RRNRQLCALQ
     LNEKRVDVVY AKEVDFSRAP NLFGLFANRR AALQALQTIA DEQKLCYGLL GLEPLSRGRA
     CFRSALKRCA GACCGKESHD DHALRLRQSL ERLRVVCWPW KGAVALKEQH PEMTQYHIIQ
     NWLWLGAVNS LKEATTLIRA PAGFDHDGYK ILCKPLLSGN YEITELDPVN DQQAS