CHO_ECOLI
ID CHO_ECOLI Reviewed; 295 AA.
AC P76213; Q2MB42;
DT 16-APR-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Excinuclease cho;
DE EC=3.1.25.-;
DE AltName: Full=Endonuclease cho;
DE AltName: Full=UvrC homolog protein;
GN Name=cho; Synonyms=dinM, sosD, ydjQ; OrderedLocusNames=b1741, JW1730;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [3]
RP REGULATION BY LEXA, AND INDUCTION.
RC STRAIN=K12 / RW118;
RX PubMed=10760155; DOI=10.1046/j.1365-2958.2000.01826.x;
RA Fernandez De Henestrosa A.R., Ogi T., Aoyagi S., Chafin D., Hayes J.J.,
RA Ohmori H., Woodgate R.;
RT "Identification of additional genes belonging to the LexA regulon in
RT Escherichia coli.";
RL Mol. Microbiol. 35:1560-1572(2000).
RN [4]
RP FUNCTION.
RX PubMed=11818552; DOI=10.1073/pnas.032584099;
RA Moolenaar G.F., van Rossum-Fikkert S., van Kesteren M., Goosen N.;
RT "Cho, a second endonuclease involved in Escherichia coli nucleotide
RT excision repair.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:1467-1472(2002).
CC -!- FUNCTION: Incises the DNA at the 3' side of a lesion during nucleotide
CC excision repair. Incises the DNA farther away from the lesion than
CC UvrC. Not able to incise the 5' site of a lesion. In vitro, the
CC incision activity of Cho is UvrA and UvrB dependent. When a lesion
CC remains because UvrC is not able to induce the 3' incision, Cho incises
CC the DNA. Then UvrC makes the 5' incision. The combined action of Cho
CC and UvrC broadens the substrate range of nucleotide excision repair.
CC {ECO:0000269|PubMed:11818552}.
CC -!- INTERACTION:
CC P76213; P0A6F5: groEL; NbExp=3; IntAct=EBI-545155, EBI-543750;
CC -!- INDUCTION: Repressed by LexA, induced by DNA damage.
CC {ECO:0000269|PubMed:10760155}.
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DR EMBL; U00096; AAC74811.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76514.1; -; Genomic_DNA.
DR PIR; E64933; E64933.
DR RefSeq; NP_416255.1; NC_000913.3.
DR RefSeq; WP_000252397.1; NZ_SSZK01000001.1.
DR AlphaFoldDB; P76213; -.
DR SMR; P76213; -.
DR BioGRID; 4261591; 124.
DR BioGRID; 853244; 1.
DR DIP; DIP-28067N; -.
DR IntAct; P76213; 8.
DR STRING; 511145.b1741; -.
DR PaxDb; P76213; -.
DR PRIDE; P76213; -.
DR EnsemblBacteria; AAC74811; AAC74811; b1741.
DR EnsemblBacteria; BAE76514; BAE76514; BAE76514.
DR GeneID; 948996; -.
DR KEGG; ecj:JW1730; -.
DR KEGG; eco:b1741; -.
DR PATRIC; fig|1411691.4.peg.515; -.
DR EchoBASE; EB3749; -.
DR eggNOG; COG0322; Bacteria.
DR HOGENOM; CLU_054721_1_0_6; -.
DR OMA; RVMSHFR; -.
DR PhylomeDB; P76213; -.
DR BioCyc; EcoCyc:G6937-MON; -.
DR PRO; PR:P76213; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0009380; C:excinuclease repair complex; IBA:GO_Central.
DR GO; GO:0004520; F:endodeoxyribonuclease activity; IDA:EcoCyc.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; IEP:EcoliWiki.
DR GO; GO:0033683; P:nucleotide-excision repair, DNA incision; IDA:EcoCyc.
DR GO; GO:0009432; P:SOS response; IEP:EcoCyc.
DR Gene3D; 3.40.1440.10; -; 1.
DR InterPro; IPR000305; GIY-YIG_endonuc.
DR InterPro; IPR035901; GIY-YIG_endonuc_sf.
DR SMART; SM00465; GIYc; 1.
DR SUPFAM; SSF82771; SSF82771; 1.
DR PROSITE; PS50164; GIY_YIG; 1.
PE 1: Evidence at protein level;
KW DNA damage; DNA excision; DNA repair; Excision nuclease; Hydrolase;
KW Reference proteome; SOS response.
FT CHAIN 1..295
FT /note="Excinuclease cho"
FT /id="PRO_0000138370"
FT DOMAIN 33..108
FT /note="GIY-YIG"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00977"
SQ SEQUENCE 295 AA; 33739 MW; DCEEADD895EF4327 CRC64;
MVRRLTSPRL EFEAAAIYEY PEHLRSFLND LPTRPGVYLF HGESDTMPLY IGKSVNIRSR
VLSHLRTPDE AAMLRQSRRI SWICTAGEIG ALLLEARLIK EQQPLFNKRL RRNRQLCALQ
LNEKRVDVVY AKEVDFSRAP NLFGLFANRR AALQALQTIA DEQKLCYGLL GLEPLSRGRA
CFRSALKRCA GACCGKESHE EHALRLRQSL ERLRVVCWPW QGAVALKEQH PEMTQYHIIQ
NWLWLGAVNS LEEATTLIRT PAGFDHDGYK ILCKPLLSGN YEITELDPAN DQRAS