CHPB_ECOLI
ID CHPB_ECOLI Reviewed; 116 AA.
AC P33647; Q2M680;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Endoribonuclease toxin ChpB;
DE EC=3.1.-.-;
DE AltName: Full=Toxin ChpB;
DE AltName: Full=mRNA interferase ChpB;
GN Name=chpB; Synonyms=chpBK, yjfE; OrderedLocusNames=b4225, JW4184;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND PROBABLE OPERON STRUCTURE.
RC STRAIN=K12 / MC1000 / ATCC 39531;
RX PubMed=8226627; DOI=10.1128/jb.175.21.6850-6856.1993;
RA Masuda Y., Miyakawa K., Nishimura Y., Ohtsubo E.;
RT "chpA and chpB, Escherichia coli chromosomal homologs of the pem locus
RT responsible for stable maintenance of plasmid R100.";
RL J. Bacteriol. 175:6850-6856(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=7610040; DOI=10.1093/nar/23.12.2105;
RA Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.;
RT "Analysis of the Escherichia coli genome VI: DNA sequence of the region
RT from 92.8 through 100 minutes.";
RL Nucleic Acids Res. 23:2105-2119(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-30.
RC STRAIN=K12;
RX PubMed=3525538; DOI=10.1016/s0021-9258(18)67610-0;
RA Weiss D.L., Johnson D.I., Weith H.L., Somerville R.L.;
RT "Structural analysis of the ileR locus of Escherichia coli K12.";
RL J. Biol. Chem. 261:9966-9971(1986).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 20-116.
RC STRAIN=K12;
RX PubMed=2848015; DOI=10.1128/jb.170.12.5901-5907.1988;
RA Lahti R., Pitkaeranta T., Valve E., Ilta I., Kukko-Kalske E., Heinonen J.;
RT "Cloning and characterization of the gene encoding inorganic
RT pyrophosphatase of Escherichia coli K-12.";
RL J. Bacteriol. 170:5901-5907(1988).
RN [7]
RP GENE MAPPING, AND DISRUPTION PHENOTYPE.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8083180; DOI=10.1128/jb.176.18.5861-5863.1994;
RA Masuda Y., Ohtsubo E.;
RT "Mapping and disruption of the chpB locus in Escherichia coli.";
RL J. Bacteriol. 176:5861-5863(1994).
RN [8]
RP FUNCTION, RNA CLEAVAGE, AND DISRUPTION PHENOTYPE.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=12972253; DOI=10.1016/s0022-2836(03)00922-7;
RA Christensen S.K., Pedersen K., Hansen F.G., Gerdes K.;
RT "Toxin-antitoxin loci as stress-response-elements: ChpAK/MazF and ChpBK
RT cleave translated RNAs and are counteracted by tmRNA.";
RL J. Mol. Biol. 332:809-819(2003).
RN [9]
RP FUNCTION AS AN RNA ENDORIBONUCLEASE, SUBUNIT, AND INTERACTION WITH CHPS.
RX PubMed=16413033; DOI=10.1016/j.jmb.2005.12.033;
RA Kamphuis M.B., Bonvin A.M., Monti M.C., Lemonnier M., Munoz-Gomez A.,
RA van den Heuvel R.H., Diaz-Orejas R., Boelens R.;
RT "Model for RNA binding and the catalytic site of the RNase Kid of the
RT bacterial parD toxin-antitoxin system.";
RL J. Mol. Biol. 357:115-126(2006).
RN [10]
RP FUNCTION, AND ACTIVITY REGULATION.
RX PubMed=21419338; DOI=10.1016/j.molcel.2011.02.023;
RA Belitsky M., Avshalom H., Erental A., Yelin I., Kumar S., London N.,
RA Sperber M., Schueler-Furman O., Engelberg-Kulka H.;
RT "The Escherichia coli extracellular death factor EDF induces the
RT endoribonucleolytic activities of the toxins MazF and ChpBK.";
RL Mol. Cell 41:625-635(2011).
RN [11]
RP RETRACTED PAPER.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=21788497; DOI=10.1073/pnas.1100186108;
RA Maisonneuve E., Shakespeare L.J., Joergensen M.G., Gerdes K.;
RT "Bacterial persistence by RNA endonucleases.";
RL Proc. Natl. Acad. Sci. U.S.A. 108:13206-13211(2011).
RN [12]
RP RETRACTION NOTICE OF PUBMED:21788497.
RX PubMed=29531044; DOI=10.1073/pnas.1803278115;
RA Maisonneuve E., Shakespeare L.J., Joergensen M.G., Gerdes K.;
RL Proc. Natl. Acad. Sci. U.S.A. 115:E2901-E2901(2018).
CC -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system.
CC ChpB is a sequence-specific mRNA and (weak) tmRNA endoribonuclease that
CC inhibits protein synthesis and induces bacterial stasis. Cleavage is
CC independent of the ribosome. Cleavage occurs at ACY sequences where Y
CC is not C. The endoribonuclease activity is not as strong as that of
CC MazF. The endoribonuclease activity (a toxin) is inhibited by its
CC labile cognate antitoxin ChpS. Toxicity results when the levels of ChpS
CC decrease in the cell, leading to mRNA degradation. Both ChpS and ChpB
CC probably bind to the promoter region of the chpS-chpB operon to
CC autoregulate their synthesis. {ECO:0000269|PubMed:12972253,
CC ECO:0000269|PubMed:16413033, ECO:0000269|PubMed:21419338,
CC ECO:0000269|PubMed:8226627}.
CC -!- ACTIVITY REGULATION: Stimulated in vitro in a concentration-dependent
CC fashion by extracellular death factor (EDF, a quorum sensing
CC pentapeptide sequence NNWNN, probably produced from the zwf gene
CC product glucose-6-phosphate 1-dehydrogenase), which is able to overcome
CC inhibition by cognate antitoxin ChpS. {ECO:0000269|PubMed:21419338}.
CC -!- SUBUNIT: Homodimer, interacts with ChpS, which inhibits the
CC endoribonuclease activity. {ECO:0000269|PubMed:16413033}.
CC -!- INDUCTION: Part of the chpS-chpB operon. {ECO:0000269|PubMed:8226627}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype (PubMed:8083180,
CC PubMed:12972253). {ECO:0000269|PubMed:12972253,
CC ECO:0000269|PubMed:8083180}.
CC -!- SIMILARITY: Belongs to the PemK/MazF family. {ECO:0000305}.
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DR EMBL; D16451; BAA03920.1; -; Genomic_DNA.
DR EMBL; M14018; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; U14003; AAA97122.1; -; Genomic_DNA.
DR EMBL; U00096; AAC77182.1; -; Genomic_DNA.
DR EMBL; M23550; -; NOT_ANNOTATED_CDS; Unassigned_DNA.
DR EMBL; AP009048; BAE78226.1; -; Genomic_DNA.
DR PIR; D49339; D49339.
DR RefSeq; NP_418646.1; NC_000913.3.
DR RefSeq; WP_000239577.1; NZ_LN832404.1.
DR AlphaFoldDB; P33647; -.
DR SMR; P33647; -.
DR BioGRID; 4259311; 4.
DR ComplexPortal; CPX-4063; ChpBS toxin-antitoxin complex.
DR STRING; 511145.b4225; -.
DR jPOST; P33647; -.
DR PaxDb; P33647; -.
DR PRIDE; P33647; -.
DR EnsemblBacteria; AAC77182; AAC77182; b4225.
DR EnsemblBacteria; BAE78226; BAE78226; BAE78226.
DR GeneID; 948747; -.
DR KEGG; ecj:JW4184; -.
DR KEGG; eco:b4225; -.
DR PATRIC; fig|511145.12.peg.4357; -.
DR EchoBASE; EB2020; -.
DR eggNOG; COG2337; Bacteria.
DR HOGENOM; CLU_121823_2_1_6; -.
DR InParanoid; P33647; -.
DR OMA; RVISMID; -.
DR PhylomeDB; P33647; -.
DR BioCyc; EcoCyc:EG12096-MON; -.
DR BioCyc; MetaCyc:EG12096-MON; -.
DR PRO; PR:P33647; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0110001; C:toxin-antitoxin complex; IPI:ComplexPortal.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0004521; F:endoribonuclease activity; IDA:UniProtKB.
DR GO; GO:0016891; F:endoribonuclease activity, producing 5'-phosphomonoesters; IDA:EcoCyc.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006402; P:mRNA catabolic process; IBA:GO_Central.
DR GO; GO:0030308; P:negative regulation of cell growth; IMP:EcoCyc.
DR GO; GO:0040008; P:regulation of growth; IC:ComplexPortal.
DR GO; GO:0043488; P:regulation of mRNA stability; IDA:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IC:ComplexPortal.
DR GO; GO:0016075; P:rRNA catabolic process; IBA:GO_Central.
DR GO; GO:0044010; P:single-species biofilm formation; IC:ComplexPortal.
DR Gene3D; 2.30.30.110; -; 1.
DR InterPro; IPR003477; PemK-like.
DR InterPro; IPR011067; Plasmid_toxin/cell-grow_inhib.
DR PANTHER; PTHR33988; PTHR33988; 1.
DR Pfam; PF02452; PemK_toxin; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Endonuclease; Hydrolase; Nuclease; Reference proteome;
KW RNA-binding; Toxin-antitoxin system.
FT CHAIN 1..116
FT /note="Endoribonuclease toxin ChpB"
FT /id="PRO_0000201899"
SQ SEQUENCE 116 AA; 12492 MW; 4D641F3E302FCF58 CRC64;
MVKKSEFERG DIVLVGFDPA SGHEQQGAGR PALVLSVQAF NQLGMTLVAP ITQGGNFARY
AGFSVPLHCE EGDVHGVVLV NQVRMMDLHA RLAKRIGLAA DEVVEEALLR LQAVVE