ACEK_SALTY
ID ACEK_SALTY Reviewed; 583 AA.
AC P51067;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2002, sequence version 2.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Isocitrate dehydrogenase kinase/phosphatase {ECO:0000255|HAMAP-Rule:MF_00747};
DE Short=IDH kinase/phosphatase {ECO:0000255|HAMAP-Rule:MF_00747};
DE Short=IDHK/P {ECO:0000255|HAMAP-Rule:MF_00747};
DE EC=2.7.11.5 {ECO:0000255|HAMAP-Rule:MF_00747};
DE EC=3.1.3.- {ECO:0000255|HAMAP-Rule:MF_00747};
GN Name=aceK {ECO:0000255|HAMAP-Rule:MF_00747}; OrderedLocusNames=STM4185;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=S2978, S2979, S2980, S2983, S2993, S2995, S3013, S3014, S3015,
RC S3027, S3041, S3044, S3057, S3333, and S4194;
RX PubMed=9409817; DOI=10.1093/genetics/147.4.1509;
RA Nelson K., Wang F.S., Boyd E.F., Selander R.K.;
RT "Size and sequence polymorphism in the isocitrate dehydrogenase
RT kinase/phosphatase gene (aceK) and flanking regions in Salmonella enterica
RT and Escherichia coli.";
RL Genetics 147:1509-1520(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
CC -!- FUNCTION: Bifunctional enzyme which can phosphorylate or
CC dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine
CC residue. This is a regulatory mechanism which enables bacteria to
CC bypass the Krebs cycle via the glyoxylate shunt in response to the
CC source of carbon. When bacteria are grown on glucose, IDH is fully
CC active and unphosphorylated, but when grown on acetate or ethanol, the
CC activity of IDH declines drastically concomitant with its
CC phosphorylation. {ECO:0000255|HAMAP-Rule:MF_00747}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[isocitrate dehydrogenase] = ADP + H(+) + O-
CC phospho-L-seryl-[isocitrate dehydrogenase]; Xref=Rhea:RHEA:43540,
CC Rhea:RHEA-COMP:10605, Rhea:RHEA-COMP:10606, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:29999, ChEBI:CHEBI:30616, ChEBI:CHEBI:83421,
CC ChEBI:CHEBI:456216; EC=2.7.11.5; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_00747};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00747}.
CC -!- SIMILARITY: Belongs to the AceK family. {ECO:0000255|HAMAP-
CC Rule:MF_00747}.
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DR EMBL; U43344; AAC43868.1; -; Genomic_DNA.
DR EMBL; U43345; AAC43870.1; -; Genomic_DNA.
DR EMBL; U43347; AAC43873.1; -; Genomic_DNA.
DR EMBL; U43348; AAC43875.1; -; Genomic_DNA.
DR EMBL; U43349; AAC43877.1; -; Genomic_DNA.
DR EMBL; U43350; AAC43879.1; -; Genomic_DNA.
DR EMBL; U43351; AAC43881.1; -; Genomic_DNA.
DR EMBL; U43352; AAC43883.1; -; Genomic_DNA.
DR EMBL; U43353; AAC43885.1; -; Genomic_DNA.
DR EMBL; U43354; AAC43887.1; -; Genomic_DNA.
DR EMBL; U43355; AAC43889.1; -; Genomic_DNA.
DR EMBL; U43356; AAC43891.1; -; Genomic_DNA.
DR EMBL; U43357; AAC43893.1; -; Genomic_DNA.
DR EMBL; U43358; AAC43895.1; -; Genomic_DNA.
DR EMBL; U43359; AAC43897.1; -; Genomic_DNA.
DR EMBL; AE006468; AAL23009.1; -; Genomic_DNA.
DR PIR; T11210; T11210.
DR RefSeq; NP_463050.1; NC_003197.2.
DR RefSeq; WP_001137266.1; NC_003197.2.
DR AlphaFoldDB; P51067; -.
DR SMR; P51067; -.
DR STRING; 99287.STM4185; -.
DR PaxDb; P51067; -.
DR PRIDE; P51067; -.
DR EnsemblBacteria; AAL23009; AAL23009; STM4185.
DR GeneID; 1255711; -.
DR KEGG; stm:STM4185; -.
DR PATRIC; fig|99287.12.peg.4398; -.
DR HOGENOM; CLU_033804_1_1_6; -.
DR OMA; EPWYSVG; -.
DR PhylomeDB; P51067; -.
DR BioCyc; SENT99287:STM4185-MON; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008772; F:[isocitrate dehydrogenase (NADP+)] kinase activity; IBA:GO_Central.
DR GO; GO:0016208; F:AMP binding; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IBA:GO_Central.
DR GO; GO:0004721; F:phosphoprotein phosphatase activity; IBA:GO_Central.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0006006; P:glucose metabolic process; IEA:InterPro.
DR GO; GO:0006097; P:glyoxylate cycle; IEA:UniProtKB-UniRule.
DR GO; GO:0018105; P:peptidyl-serine phosphorylation; IBA:GO_Central.
DR GO; GO:0006470; P:protein dephosphorylation; IBA:GO_Central.
DR GO; GO:0050790; P:regulation of catalytic activity; IBA:GO_Central.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00747; AceK; 1.
DR InterPro; IPR010452; Isocitrate_DH_AceK.
DR PANTHER; PTHR39559; PTHR39559; 1.
DR Pfam; PF06315; AceK; 1.
DR PIRSF; PIRSF000719; AceK; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cytoplasm; Glyoxylate bypass; Hydrolase; Kinase;
KW Nucleotide-binding; Protein phosphatase; Reference proteome;
KW Serine/threonine-protein kinase; Transferase; Tricarboxylic acid cycle.
FT CHAIN 1..583
FT /note="Isocitrate dehydrogenase kinase/phosphatase"
FT /id="PRO_0000057909"
FT ACT_SITE 371
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00747"
FT BINDING 315..321
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00747"
FT BINDING 336
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00747"
FT VARIANT 2
FT /note="P -> S (in strain: S2980 and S2983)"
FT VARIANT 4
FT /note="G -> C (in strain: S2978 and S2979)"
FT VARIANT 20
FT /note="Q -> R (in strain: S3333)"
FT VARIANT 41
FT /note="H -> Q (in strain: S2978 and S2979)"
FT VARIANT 73
FT /note="S -> N (in strain: S2980 and S2983)"
FT VARIANT 77
FT /note="D -> N (in strain: S2980, S2983, S3013, S3014, S3015
FT and S3027)"
FT VARIANT 146..147
FT /note="DH -> EQ (in strain: S3041 and S3044)"
FT VARIANT 151
FT /note="P -> L (in strain: S2980 and S2983)"
FT VARIANT 151
FT /note="P -> S (in strain: S2978, S2979 and S2993)"
FT VARIANT 154
FT /note="M -> V (in strain: S3041 and S3044)"
FT VARIANT 156
FT /note="I -> M (in strain: S3044)"
FT VARIANT 163
FT /note="R -> C (in strain: S3333)"
FT VARIANT 167
FT /note="Q -> L (in strain: S3015 and S3027)"
FT VARIANT 170
FT /note="S -> R (in strain: S3013, S3014, S3015 and S3027)"
FT VARIANT 174
FT /note="R -> H (in strain: S3041 and S3044)"
FT VARIANT 181
FT /note="T -> A (in strain: S3015 and S3027)"
FT VARIANT 183
FT /note="T -> A (in strain: S3015 and S3027)"
FT VARIANT 183
FT /note="T -> I (in strain: S2980 and S2983)"
FT VARIANT 184
FT /note="L -> F (in strain: S3041 and S3044)"
FT VARIANT 186..188
FT /note="EDA -> AEG (in strain: S3041 and S3044)"
FT VARIANT 188
FT /note="A -> G (in strain: S3014)"
FT VARIANT 194
FT /note="V -> L (in strain: S2980, S2983, S3041 and S3044)"
FT VARIANT 197
FT /note="A -> T (in strain: S3041)"
FT VARIANT 213..214
FT /note="LT -> FI (in strain: S3041 and S3044)"
FT VARIANT 214
FT /note="T -> A (in strain: S2980 and S2983)"
FT VARIANT 303
FT /note="C -> G (in strain: S3041 and S3044)"
FT VARIANT 307
FT /note="E -> N (in strain: S3041 and S3044)"
FT VARIANT 348
FT /note="M -> V (in strain: S3041 and S3044)"
FT VARIANT 383..384
FT /note="RQ -> QR (in strain: S3041 and S3044)"
FT VARIANT 391
FT /note="A -> T (in strain: S2995 and S3057)"
FT VARIANT 394
FT /note="R -> Q (in strain: S2980, S2983, S3041 and S3044)"
FT VARIANT 407
FT /note="H -> Q (in strain: S2978, S2979, S2993, S2995,
FT S3013, S3014, S3015, S3027, S3041, S3044 and S3057)"
FT VARIANT 409
FT /note="V -> A (in strain: S3041 and S3044)"
FT VARIANT 411
FT /note="R -> C (in strain: S2993)"
FT VARIANT 429
FT /note="V -> A (in strain: S3041 and S3044)"
FT VARIANT 486
FT /note="N -> H (in strain: S3041 and S3044)"
FT VARIANT 492
FT /note="P -> T (in strain: S3044)"
FT VARIANT 501
FT /note="A -> S (in strain: S2979)"
FT VARIANT 502
FT /note="S -> C (in strain: S3041 and S3044)"
FT VARIANT 510..511
FT /note="PG -> RC (in strain: S2979)"
FT VARIANT 547
FT /note="A -> G (in strain: S3041 and S3044)"
FT VARIANT 554
FT /note="E -> N (in strain: S3041 and S3044)"
FT VARIANT 557
FT /note="V -> I (in strain: S2978, S2979 and S2993)"
FT VARIANT 570
FT /note="S -> G (in strain: S2978, S2979, S2995 and S3057)"
FT VARIANT 574..583
FT /note="GAISSTANSS -> DIDRRLDKAPAPAGNVCRTA (in strain: S2980
FT and S2983)"
FT VARIANT 574..583
FT /note="GAISSTANSS -> ETDRRPDKAFAPPSGNVRRA (in strain:
FT S3333)"
FT VARIANT 576..583
FT /note="ISSTANSS -> KEASPL (in strain: S2995 and S3057)"
FT VARIANT 578
FT /note="S -> T (in strain: S3041 and S3044)"
FT VARIANT 580
FT /note="A -> V (in strain: S3015, S3027, S3041 and S3044)"
SQ SEQUENCE 583 AA; 67956 MW; 6214DEB7D8ACD04E CRC64;
MPRGLELLIA QTILQGFDAQ YGRFLEVTSG AQQRFEQADW HAVQQAMKSR IHLYDHHVGL
VVEQLRCITD GKSTDADFLL RVKEHYTRLL PDYPRFEIAE SFFNSVYCRL FDHRSLTPER
LFIFSSQPER RFRTIPRPLA KDFFPDHGWE PLLMRILSDL PLRLPWQNKS RDIRYIIAHL
TETLGEDALP RCHVQVANEL FYRNKAAWLV GKLTTPDGTL PFLLPIHRTD EGELFVDTCL
TTTAEASIVF GFARSYFMVY APLPAALVEW LREILPGKTT AELYMAIGCQ KHAKTESYRE
YLCYLAESDE KFIEAPGIRG MVMLVFTLPG FDRVFKIIKD KFAPQKEMSA AHVRACYQLV
KEHDRVGRMA DTQEFENFVL DKRQIDPALM ALLRQEAPEK ITDLGEHIVI RHLYIERRMV
PLNIWLEQVE GQQLRDAIEE YGNAIRQLAA ANIFPGDMLF KNFGVTRHGR VVFYDYDEIC
YMTEVNFRDI PPARYPEDEL ASEPWYSVSP GDVFPEEFRH WLCADPRIGP LFEEMHADLF
RADYWRALQT RIKEGHVEDV YAYRRRQRFS VRYGAISSTA NSS
