CHRA1_CUPMC
ID CHRA1_CUPMC Reviewed; 401 AA.
AC P17551; Q1L9W5; Q5NV00;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1990, sequence version 1.
DT 25-MAY-2022, entry version 129.
DE RecName: Full=Chromate transport protein {ECO:0000305};
GN Name=chrA1 {ECO:0000312|EMBL:CAC42411.1};
GN Synonyms=chrA {ECO:0000303|PubMed:2180932};
GN OrderedLocusNames=Rmet_6202 {ECO:0000312|EMBL:ABF13061.1};
GN ORFNames=RMe0090 {ECO:0000312|EMBL:CAI30234.1};
OS Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 /
OS CH34) (Ralstonia metallidurans).
OG Plasmid pMOL28.
OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Cupriavidus.
OX NCBI_TaxID=266264;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX PubMed=2180932; DOI=10.1016/s0021-9258(19)39411-6;
RA Nies A., Nies D.H., Silver S.;
RT "Nucleotide sequence and expression of a plasmid-encoded chromate
RT resistance determinant from Alcaligenes eutrophus.";
RL J. Biol. Chem. 265:5648-5653(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA van der Lelie D., Monchy S., Taghavi S., McCorkle S., Dunn J.,
RA Benotmane M., Vallaeys T., Lapidus A., Mergeay M.;
RT "Sequence and features of the Ralstonia metallidurans CH34 heavy metal
RT plasmids pMOL28 and pMOL30.";
RL Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43123 / DSM 2839 / NBRC 102507 / CH34;
RX PubMed=20463976; DOI=10.1371/journal.pone.0010433;
RA Janssen P.J., Van Houdt R., Moors H., Monsieurs P., Morin N., Michaux A.,
RA Benotmane M.A., Leys N., Vallaeys T., Lapidus A., Monchy S., Medigue C.,
RA Taghavi S., McCorkle S., Dunn J., van der Lelie D., Mergeay M.;
RT "The complete genome sequence of Cupriavidus metallidurans strain CH34, a
RT master survivalist in harsh and anthropogenic environments.";
RL PLoS ONE 5:E10433-E10433(2010).
RN [4]
RP INDUCTION.
RX PubMed=12471500; DOI=10.1007/s00203-002-0492-5;
RA Juhnke S., Peitzsch N., Huebener N., Grosse C., Nies D.H.;
RT "New genes involved in chromate resistance in Ralstonia metallidurans
RT strain CH34.";
RL Arch. Microbiol. 179:15-25(2002).
CC -!- FUNCTION: This protein reduces chromate accumulation and is essential
CC for chromate resistance. {ECO:0000269|PubMed:2180932}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000255}.
CC -!- INDUCTION: By chromate; induction increases when cells are grown in the
CC presence of high sulfate concentrations (3 mM SO4(2-)).
CC {ECO:0000269|PubMed:12471500}.
CC -!- SIMILARITY: Belongs to the chromate ion transporter (CHR) (TC 2.A.51)
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAI30234.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AJ313327; CAC42411.1; -; Genomic_DNA.
DR EMBL; X90708; CAI30234.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP000355; ABF13061.1; -; Genomic_DNA.
DR PIR; B35177; B35177.
DR RefSeq; WP_011514877.1; NC_007972.2.
DR RefSeq; YP_161712.1; NC_006525.1.
DR AlphaFoldDB; P17551; -.
DR TCDB; 2.A.51.1.1; the chromate ion transporter (chr) family.
DR EnsemblBacteria; ABF13061; ABF13061; Rmet_6202.
DR GeneID; 60825781; -.
DR KEGG; rme:Rmet_6202; -.
DR HOGENOM; CLU_018106_0_0_4; -.
DR OMA; CMFFGCL; -.
DR OrthoDB; 1884249at2; -.
DR Proteomes; UP000002429; Plasmid pMOL28.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015109; F:chromate transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0046687; P:response to chromate; IEA:UniProtKB-KW.
DR InterPro; IPR014047; Chr_Tranpt_l_chain.
DR InterPro; IPR003370; Chromate_transpt.
DR Pfam; PF02417; Chromate_transp; 2.
DR PIRSF; PIRSF004810; ChrA; 1.
DR TIGRFAMs; TIGR00937; 2A51; 1.
PE 2: Evidence at transcript level;
KW Cell inner membrane; Cell membrane; Chromate resistance; Membrane; Plasmid;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..401
FT /note="Chromate transport protein"
FT /id="PRO_0000089654"
FT TRANSMEM 26..46
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 67..87
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 93..113
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 124..144
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 172..192
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 214..234
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 237..257
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 272..294
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 299..321
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 330..350
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 356..376
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 379..399
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 401 AA; 42642 MW; 6DC963FBADA6DBC5 CRC64;
MNSPQPPDTT AAGSVHTAPT YTLRQLVMYF LRLGTLGFGG PVALAGYMHR DLVEAKQWIT
DADYKEGLAL AQLAPGPLAA QLAIYLGYVH YRIVGATLVG VAFVLPSFLM VLALGWAYVR
FGGLTWMQSV FYGVGAAVIG IIAISAYKLT KKSVGNDKLL WFIYLVLVAV TVITESEVAW
LFLAAGVLVW FWRAPPKWLR QGKMNAFAAT PLPAASGMMS TLDWPLLSQI GVFFAKAGAF
VFGSGLAIVP FLYGGVVTEY HWLNDKQFVD AVAVAMITPG PVVITVGFIG YLVAGLPGAC
VAAAATFLPC YLFTVLPAPY FKKYGKLPAI LAFVDGVTAA AIGAITGAVI VLAKRSIVDI
PTALLALVTV ALLLKFKKLS EPMIVAGAAL IGLVAYPLLH H
