CHRB_DROME
ID CHRB_DROME Reviewed; 299 AA.
AC Q9VTI8; Q9NHN5;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 2.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Protein charybde;
DE AltName: Full=Protein charybdis;
DE AltName: Full=Protein regulated in development and DNA damage response 1;
DE Short=REDD-1;
GN Name=chrb; Synonyms=char; ORFNames=CG7533;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Chauvet S., Maurel-Zaffran C., Miassod R., Jullien N., Pradel J.,
RA Aragnol D.;
RT "Characterization of charybde and scylla, two paralogous target genes of
RT Hox and cofactors proteins in Drosophila.";
RL Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP IDENTIFICATION.
RX PubMed=12453409; DOI=10.1016/s1097-2765(02)00706-2;
RA Ellisen L.W., Ramsayer K.D., Johannessen C.M., Yang A., Beppu H., Minda K.,
RA Oliner J.D., McKeon F., Haber D.A.;
RT "REDD1, a developmentally regulated transcriptional target of p63 and p53,
RT links p63 to regulation of reactive oxygen species.";
RL Mol. Cell 10:995-1005(2002).
RN [6]
RP FUNCTION, AND INDUCTION.
RX PubMed=15545626; DOI=10.1101/gad.322704;
RA Reiling J.H., Hafen E.;
RT "The hypoxia-induced paralogs Scylla and Charybdis inhibit growth by down-
RT regulating S6K activity upstream of TSC in Drosophila.";
RL Genes Dev. 18:2879-2892(2004).
RN [7]
RP FUNCTION.
RX PubMed=15632201; DOI=10.1074/jbc.c400557200;
RA Corradetti M.N., Inoki K., Guan K.-L.;
RT "The stress-inducted proteins RTP801 and RTP801L are negative regulators of
RT the mammalian target of rapamycin pathway.";
RL J. Biol. Chem. 280:9769-9772(2005).
RN [8]
RP FUNCTION, INDUCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=16423342; DOI=10.1016/j.ydbio.2005.12.014;
RA Scuderi A., Simin K., Kazuko S.G., Metherall J.E., Letsou A.;
RT "scylla and charybde, homologues of the human apoptotic gene RTP801, are
RT required for head involution in Drosophila.";
RL Dev. Biol. 291:110-122(2006).
CC -!- FUNCTION: Inhibits cell growth by regulating the Tor pathway upstream
CC of the Tsc1-Tsc2 complex and downstream of Akt1. Acts as cell death
CC activator during head development. {ECO:0000269|PubMed:15545626,
CC ECO:0000269|PubMed:15632201, ECO:0000269|PubMed:16423342}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- DEVELOPMENTAL STAGE: Expression peaks 2-8 hours after egg laying.
CC Expressed in the dorsal domains of gastrulation stage embryos. During
CC mid-embryonic development, expressed in the central nervous system, the
CC three thoracic segments and the cardiac precursor cells.
CC {ECO:0000269|PubMed:16423342}.
CC -!- INDUCTION: Expression is controlled by homeobox transcription factors.
CC At larval stages, induced by starvation, and mildly by hypoxia in
CC midgut but not fat body. {ECO:0000269|PubMed:15545626,
CC ECO:0000269|PubMed:16423342}.
CC -!- SIMILARITY: Belongs to the DDIT4 family. {ECO:0000305}.
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DR EMBL; AF221109; AAF59840.1; -; mRNA.
DR EMBL; AE014296; AAF50060.2; -; Genomic_DNA.
DR EMBL; AY060367; AAL25406.1; -; mRNA.
DR RefSeq; NP_648470.2; NM_140213.5.
DR AlphaFoldDB; Q9VTI8; -.
DR SMR; Q9VTI8; -.
DR BioGRID; 64656; 5.
DR IntAct; Q9VTI8; 6.
DR STRING; 7227.FBpp0304225; -.
DR PaxDb; Q9VTI8; -.
DR PRIDE; Q9VTI8; -.
DR DNASU; 39284; -.
DR EnsemblMetazoa; FBtr0076093; FBpp0099774; FBgn0036165.
DR GeneID; 39284; -.
DR KEGG; dme:Dmel_CG7533; -.
DR CTD; 39284; -.
DR FlyBase; FBgn0036165; chrb.
DR VEuPathDB; VectorBase:FBgn0036165; -.
DR eggNOG; ENOG502R3EE; Eukaryota.
DR GeneTree; ENSGT00530000063652; -.
DR InParanoid; Q9VTI8; -.
DR OrthoDB; 1588396at2759; -.
DR PhylomeDB; Q9VTI8; -.
DR Reactome; R-DME-5628897; TP53 Regulates Metabolic Genes.
DR SignaLink; Q9VTI8; -.
DR BioGRID-ORCS; 39284; 0 hits in 3 CRISPR screens.
DR ChiTaRS; chrb; fly.
DR GenomeRNAi; 39284; -.
DR PRO; PR:Q9VTI8; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0036165; Expressed in antenna and 64 other tissues.
DR ExpressionAtlas; Q9VTI8; baseline and differential.
DR Genevisible; Q9VTI8; DM.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0006915; P:apoptotic process; IMP:UniProtKB.
DR GO; GO:0008219; P:cell death; IMP:FlyBase.
DR GO; GO:0008258; P:head involution; IMP:UniProtKB.
DR GO; GO:0045926; P:negative regulation of growth; IGI:FlyBase.
DR GO; GO:0009968; P:negative regulation of signal transduction; IEA:InterPro.
DR GO; GO:0032006; P:regulation of TOR signaling; IMP:UniProtKB.
DR GO; GO:0006979; P:response to oxidative stress; IMP:UniProtKB.
DR Gene3D; 3.90.470.40; -; 1.
DR InterPro; IPR012918; RTP801-like.
DR InterPro; IPR038281; RTP801-like_C_sf.
DR PANTHER; PTHR12478; PTHR12478; 1.
DR Pfam; PF07809; RTP801_C; 1.
PE 2: Evidence at transcript level;
KW Apoptosis; Cytoplasm; Developmental protein; Reference proteome.
FT CHAIN 1..299
FT /note="Protein charybde"
FT /id="PRO_0000307209"
FT REGION 73..103
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 154
FT /note="C -> G (in Ref. 1; AAF59840)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 299 AA; 32179 MW; CD224D3BFBF315E7 CRC64;
MKMEVLSVQN HIQGKFGVNK IKDWQASTAP LEEEEELTAG VNGNTAAGEG ILDVDVVDGH
PASVLHMRQH QALNTRPSAT PPSAGGGGPL AGGGSVGMTT PKQATSPVAA ASFEAPLSGG
SAAAYHHAYM TNVLSSTAQQ HHPLPASPLQ STACARFGAA DNLDDVSASA VRELSQQLQA
QLRDAKRRHL ACTEVTLPND LTQRIAAEII RMSEREPCGE RACTLFIEFE SEPNKVKRIA
YFKVDPDTVS IFELYLTLRQ DKSGWSSLVP QFIKNLTRSN TINISPDFTL TKKKLYSSE
