CHRD_RAT
ID CHRD_RAT Reviewed; 951 AA.
AC Q63148;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 20-MAR-2007, sequence version 2.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Chordin;
DE Flags: Precursor;
GN Name=Chrd;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Brown Norway;
RX PubMed=15057822; DOI=10.1038/nature02426;
RA Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J.,
RA Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G.,
RA Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G.,
RA Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G.,
RA Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S.,
RA Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T.,
RA Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D.,
RA Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L.,
RA Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D.,
RA Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M.,
RA Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C.,
RA Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J.,
RA Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H.,
RA Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X.,
RA Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q.,
RA Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P.,
RA Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A.,
RA Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C.,
RA Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J.,
RA Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J.,
RA Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F.,
RA Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A.,
RA Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A.,
RA Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J.,
RA Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E.,
RA Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M.,
RA Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C.,
RA Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L.,
RA Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W.,
RA Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y.,
RA Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V.,
RA Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M.,
RA Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S.,
RA Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B.,
RA Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R.,
RA Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J.,
RA Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D.,
RA Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S.,
RA Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S.,
RA Mockrin S., Collins F.S.;
RT "Genome sequence of the Brown Norway rat yields insights into mammalian
RT evolution.";
RL Nature 428:493-521(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 653-828.
RC STRAIN=Sprague-Dawley; TISSUE=Brain;
RA Kuroda S., Tokunaga C., Konishi H., Kikkawa U.;
RL Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Dorsalizing factor. Key developmental protein that dorsalizes
CC early vertebrate embryonic tissues by binding to ventralizing TGF-beta
CC family bone morphogenetic proteins (BMPs) and sequestering them in
CC latent complexes (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with TWSG1 and/or BMP4. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- PTM: Cleaved by tolloid proteases; cleavage participates in
CC dorsoventral patterning during early development. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the chordin family. {ECO:0000305}.
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DR EMBL; AABR03079283; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; D86581; BAA13128.1; -; mRNA.
DR AlphaFoldDB; Q63148; -.
DR SMR; Q63148; -.
DR STRING; 10116.ENSRNOP00000002394; -.
DR GlyGen; Q63148; 3 sites.
DR PaxDb; Q63148; -.
DR PRIDE; Q63148; -.
DR RGD; 620181; Chrd.
DR eggNOG; ENOG502QR4J; Eukaryota.
DR HOGENOM; CLU_008477_0_0_1; -.
DR InParanoid; Q63148; -.
DR PhylomeDB; Q63148; -.
DR PRO; PR:Q63148; -.
DR Proteomes; UP000002494; Unplaced.
DR Genevisible; Q63148; RN.
DR GO; GO:0009986; C:cell surface; ISO:RGD.
DR GO; GO:0005576; C:extracellular region; ISO:RGD.
DR GO; GO:0005615; C:extracellular space; ISO:RGD.
DR GO; GO:0098793; C:presynapse; IEA:GOC.
DR GO; GO:0036122; F:BMP binding; ISO:RGD.
DR GO; GO:0043395; F:heparan sulfate proteoglycan binding; ISO:RGD.
DR GO; GO:0008201; F:heparin binding; ISO:RGD.
DR GO; GO:0045545; F:syndecan binding; ISO:RGD.
DR GO; GO:0048844; P:artery morphogenesis; ISO:RGD.
DR GO; GO:0030509; P:BMP signaling pathway; ISO:RGD.
DR GO; GO:0061312; P:BMP signaling pathway involved in heart development; ISO:RGD.
DR GO; GO:0021919; P:BMP signaling pathway involved in spinal cord dorsal/ventral patterning; ISO:RGD.
DR GO; GO:0008283; P:cell population proliferation; ISO:RGD.
DR GO; GO:0007417; P:central nervous system development; ISO:RGD.
DR GO; GO:1904888; P:cranial skeletal system development; ISO:RGD.
DR GO; GO:0035906; P:descending aorta development; ISO:RGD.
DR GO; GO:0009953; P:dorsal/ventral pattern formation; ISO:RGD.
DR GO; GO:0000578; P:embryonic axis specification; ISO:RGD.
DR GO; GO:0035050; P:embryonic heart tube development; ISO:RGD.
DR GO; GO:0072148; P:epithelial cell fate commitment; ISO:RGD.
DR GO; GO:0035640; P:exploration behavior; ISO:RGD.
DR GO; GO:0008543; P:fibroblast growth factor receptor signaling pathway; ISO:RGD.
DR GO; GO:0030900; P:forebrain development; ISO:RGD.
DR GO; GO:0001702; P:gastrulation with mouth forming second; ISO:RGD.
DR GO; GO:0001701; P:in utero embryonic development; ISO:RGD.
DR GO; GO:0060291; P:long-term synaptic potentiation; ISO:RGD.
DR GO; GO:0045185; P:maintenance of protein location; ISO:RGD.
DR GO; GO:0014030; P:mesenchymal cell fate commitment; ISO:RGD.
DR GO; GO:0001707; P:mesoderm formation; ISO:RGD.
DR GO; GO:0030514; P:negative regulation of BMP signaling pathway; ISO:RGD.
DR GO; GO:0030336; P:negative regulation of cell migration; ISO:RGD.
DR GO; GO:0045668; P:negative regulation of osteoblast differentiation; ISO:RGD.
DR GO; GO:0014029; P:neural crest formation; ISO:RGD.
DR GO; GO:0048663; P:neuron fate commitment; ISO:RGD.
DR GO; GO:0001649; P:osteoblast differentiation; ISO:RGD.
DR GO; GO:0007389; P:pattern specification process; ISO:RGD.
DR GO; GO:0045785; P:positive regulation of cell adhesion; ISO:RGD.
DR GO; GO:0002053; P:positive regulation of mesenchymal cell proliferation; ISO:RGD.
DR GO; GO:0099171; P:presynaptic modulation of chemical synaptic transmission; ISO:RGD.
DR GO; GO:0017038; P:protein import; ISO:RGD.
DR GO; GO:0048168; P:regulation of neuronal synaptic plasticity; ISO:RGD.
DR GO; GO:1990926; P:short-term synaptic potentiation; ISO:RGD.
DR GO; GO:0001501; P:skeletal system development; ISO:RGD.
DR GO; GO:0007224; P:smoothened signaling pathway; ISO:RGD.
DR GO; GO:0050808; P:synapse organization; ISO:RGD.
DR GO; GO:0048845; P:venous blood vessel morphogenesis; ISO:RGD.
DR GO; GO:0008542; P:visual learning; ISO:RGD.
DR InterPro; IPR016353; Chordin.
DR InterPro; IPR010895; CHRD.
DR InterPro; IPR001007; VWF_dom.
DR Pfam; PF07452; CHRD; 2.
DR Pfam; PF00093; VWC; 3.
DR PIRSF; PIRSF002496; Chordin; 1.
DR SMART; SM00754; CHRD; 4.
DR SMART; SM00214; VWC; 4.
DR PROSITE; PS50933; CHRD; 4.
DR PROSITE; PS01208; VWFC_1; 2.
DR PROSITE; PS50184; VWFC_2; 2.
PE 2: Evidence at transcript level;
KW Developmental protein; Glycoprotein; Reference proteome; Repeat; Secreted;
KW Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..951
FT /note="Chordin"
FT /id="PRO_0000219088"
FT DOMAIN 49..126
FT /note="VWFC 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT DOMAIN 171..280
FT /note="CHRD 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00230"
FT DOMAIN 282..401
FT /note="CHRD 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00230"
FT DOMAIN 402..523
FT /note="CHRD 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00230"
FT DOMAIN 529..649
FT /note="CHRD 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00230"
FT DOMAIN 702..762
FT /note="VWFC 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT DOMAIN 782..848
FT /note="VWFC 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT DOMAIN 870..930
FT /note="VWFC 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT REGION 144..170
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 669..701
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 932..951
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 145..170
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 350
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 433
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 880
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 746..749
FT /note="SCPH -> RCSQ (in Ref. 2; BAA13128)"
FT /evidence="ECO:0000305"
FT CONFLICT 757
FT /note="C -> W (in Ref. 2; BAA13128)"
FT /evidence="ECO:0000305"
FT CONFLICT 762
FT /note="P -> S (in Ref. 2; BAA13128)"
FT /evidence="ECO:0000305"
FT CONFLICT 771
FT /note="P -> S (in Ref. 2; BAA13128)"
FT /evidence="ECO:0000305"
FT CONFLICT 810
FT /note="A -> G (in Ref. 2; BAA13128)"
FT /evidence="ECO:0000305"
FT CONFLICT 817..818
FT /note="AT -> VN (in Ref. 2; BAA13128)"
FT /evidence="ECO:0000305"
FT CONFLICT 823
FT /note="C -> S (in Ref. 2; BAA13128)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 951 AA; 102007 MW; F7E2F509718C0EBE CRC64;
MPSLPAPPAP RLLLGLLLLG SRPAGGTGPE APALPIRSEK EPLPVRGAAG CSFGGKVYAL
DETWHPDLGE PFGVMRCVLC ACEAPQWARR GRGPGRVSCK NIKPQCPTLA CRQPRQLPGH
CCQTCPQDAE RSNLDPQPAG LVFEYPRDPE HRSYSDRGEP GTGERTRGDG HTEDFVALLT
GPRTQAVARA RVSLLRSSLR FSISYQRLDR PSRVRFTDPT GNILFEHPAA PTQDGLVCGV
WRAVPRLSVR LLRAEQLRVA LVTPTHPSEE VWGPLIWQGA LTAETFSAIL TLEDPLQRGV
GGIALLTLSD TEDALHFLLL FRGLLGGLAH VPLKLQILHQ GQLLRELQAN ASAQEPGFAE
VLPSLTDQEM DWLVLGELQM VLEKMGGPEL RISGYITTRQ SCDVLQSVLC GADALIPVQT
GAAGSASFIL LGNGSLIYQV QVIGTGSEVV AMTLETKPQR KNQRTVLCHM AGLQLGGHMA
VGVCSGLGAR GAHMLLQNEL FLNIGTKDFP DGELRGHVTA LCYSGHSAHY DRLPVPLAGA
LVLPPVRSQA AGHAWLSLDT HCHLHYEVLL AGLGGSEQGT VTAHLLGPPG MPGPQRLLKG
FYGSEAQGVV KDLEPVLLRH LTQGTASLLI TTKSNPRGEL RGQVHIASQC EVGGLRLASE
GVRMSLAPNG EAATSPMLPA GPGPEAPVPA KHGSSGRPRD PNTCFFEGQQ RPHGARWAPN
YDPLCSLCTC QRRTVICDPV VCPPPSCPHP VQALDQCCPV CPEKQRSRDL PSLPNLEPGE
GCYFDGDRSW RAAGTRWHPV VPPFGLIKCA VCTCKGATGE VHCEKVQCPR LACAQPVRAN
PTDCCKQCPV GSGTHAKLGD PMQADGPRGC RFAGQWFPEN QSWHPSVPPF GEMSCITCRC
GAGVPHCERD DCSPPLSCGS GKESRCCSHC TAQRSSETRT LPELEKEAQR S
