CHRY3_GIBZE
ID CHRY3_GIBZE Reviewed; 379 AA.
AC A0A098E171; A0A0E0SN27;
DT 17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT 07-JAN-2015, sequence version 1.
DT 25-MAY-2022, entry version 30.
DE RecName: Full=Oxidoreductase chry3 {ECO:0000303|PubMed:28708398};
DE EC=1.-.-.- {ECO:0000305|PubMed:28708398};
DE AltName: Full=Chrysogine biosynthesis cluster protein 3 {ECO:0000303|PubMed:28708398};
GN Name=chry3 {ECO:0000303|PubMed:28708398};
GN ORFNames=FG11397, FGRAMPH1_01T21963;
OS Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084
OS / PH-1) (Wheat head blight fungus) (Fusarium graminearum).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium.
OX NCBI_TaxID=229533;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1;
RX PubMed=17823352; DOI=10.1126/science.1143708;
RA Cuomo C.A., Gueldener U., Xu J.-R., Trail F., Turgeon B.G., Di Pietro A.,
RA Walton J.D., Ma L.-J., Baker S.E., Rep M., Adam G., Antoniw J., Baldwin T.,
RA Calvo S.E., Chang Y.-L., DeCaprio D., Gale L.R., Gnerre S., Goswami R.S.,
RA Hammond-Kosack K., Harris L.J., Hilburn K., Kennell J.C., Kroken S.,
RA Magnuson J.K., Mannhaupt G., Mauceli E.W., Mewes H.-W., Mitterbauer R.,
RA Muehlbauer G., Muensterkoetter M., Nelson D., O'Donnell K., Ouellet T.,
RA Qi W., Quesneville H., Roncero M.I.G., Seong K.-Y., Tetko I.V., Urban M.,
RA Waalwijk C., Ward T.J., Yao J., Birren B.W., Kistler H.C.;
RT "The Fusarium graminearum genome reveals a link between localized
RT polymorphism and pathogen specialization.";
RL Science 317:1400-1402(2007).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1;
RX PubMed=20237561; DOI=10.1038/nature08850;
RA Ma L.-J., van der Does H.C., Borkovich K.A., Coleman J.J., Daboussi M.-J.,
RA Di Pietro A., Dufresne M., Freitag M., Grabherr M., Henrissat B.,
RA Houterman P.M., Kang S., Shim W.-B., Woloshuk C., Xie X., Xu J.-R.,
RA Antoniw J., Baker S.E., Bluhm B.H., Breakspear A., Brown D.W.,
RA Butchko R.A.E., Chapman S., Coulson R., Coutinho P.M., Danchin E.G.J.,
RA Diener A., Gale L.R., Gardiner D.M., Goff S., Hammond-Kosack K.E.,
RA Hilburn K., Hua-Van A., Jonkers W., Kazan K., Kodira C.D., Koehrsen M.,
RA Kumar L., Lee Y.-H., Li L., Manners J.M., Miranda-Saavedra D.,
RA Mukherjee M., Park G., Park J., Park S.-Y., Proctor R.H., Regev A.,
RA Ruiz-Roldan M.C., Sain D., Sakthikumar S., Sykes S., Schwartz D.C.,
RA Turgeon B.G., Wapinski I., Yoder O., Young S., Zeng Q., Zhou S.,
RA Galagan J., Cuomo C.A., Kistler H.C., Rep M.;
RT "Comparative genomics reveals mobile pathogenicity chromosomes in
RT Fusarium.";
RL Nature 464:367-373(2010).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1;
RX PubMed=26198851; DOI=10.1186/s12864-015-1756-1;
RA King R., Urban M., Hammond-Kosack M.C.U., Hassani-Pak K.,
RA Hammond-Kosack K.E.;
RT "The completed genome sequence of the pathogenic ascomycete fungus Fusarium
RT graminearum.";
RL BMC Genomics 16:544-544(2015).
RN [4]
RP FUNCTION, AND PATHWAY.
RX PubMed=28708398; DOI=10.1021/acs.jnatprod.6b00822;
RA Wollenberg R.D., Saei W., Westphal K.R., Klitgaard C.S., Nielsen K.L.,
RA Lysoee E., Gardiner D.M., Wimmer R., Sondergaard T.E., Soerensen J.L.;
RT "Chrysogine biosynthesis is mediated by a two-module nonribosomal peptide
RT synthetase.";
RL J. Nat. Prod. 80:2131-2135(2017).
CC -!- FUNCTION: Oxidoreductase; part of the gene cluster that mediates the
CC biosynthesis of the yellow pigment chrysogine (PubMed:28708398).
CC Pyruvic acid and anthranilic acid are likely substrates for the
CC nonribosomal peptide synthetase chry1/NRPS14, with pyruvic acid
CC adenylated by the first A domain and anthranilic acid by the second
CC (Probable). If pyruvic acid and anthranilic acid are merged and
CC released from chry1/NRPS14 by hydrolysis, a subsequent amidation would
CC lead to 2-pyruvoylaminobenzamide (Probable). This process is probably
CC catalyzed by the amidotransferase chry2 using glutamine as amino donor
CC (Probable). The dehydrogenase chry5 that has a terminal berberine
CC bridge domain for C-N cyclization could catalyze the cyclization of 2-
CC pyruvoylaminobenzamide to yield acetyl-4(3H)-quinazolidinone
CC (Probable). A final reduction of acetyl-4(3H)-quinazolidinone catalyzed
CC by the oxidoreductase chry4 would result in chrysogine (Probable).
CC {ECO:0000269|PubMed:28708398, ECO:0000305|PubMed:28708398}.
CC -!- PATHWAY: Pigment biosynthesis. {ECO:0000305|PubMed:28708398}.
CC -!- SIMILARITY: Belongs to the asaB hydroxylase/desaturase family.
CC {ECO:0000305}.
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DR EMBL; HG970334; CEF87840.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A098E171; -.
DR SMR; A0A098E171; -.
DR VEuPathDB; FungiDB:FGRAMPH1_01G21963; -.
DR eggNOG; ENOG502SKC4; Eukaryota.
DR Proteomes; UP000070720; Chromosome 3.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR InterPro; IPR044053; AsaB-like.
DR PANTHER; PTHR34598; PTHR34598; 1.
PE 3: Inferred from homology;
KW Oxidoreductase; Reference proteome.
FT CHAIN 1..379
FT /note="Oxidoreductase chry3"
FT /id="PRO_0000450174"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 126..150
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 379 AA; 42272 MW; 8B563DAE25F81303 CRC64;
MTTATKPHQG RFRYLTRGSQ PTPSKEAYLL PSLSEFGDIV TLPLTDLRPS LDLGQDSPYK
LDVHGFTARR HDSALHSAPY SRASWNNEKL LRQIYFPEVE EFVKNVTGCK KAVVSAAVVR
NRLYSEGDDS APAEEEADTQ ASSSDDTSHM FPPIFGNSVK DGVCPAPKVH LDCTPKGARH
HIRRYHSEVA LAAEKVIEAE NRLLESGVEW NDLKDHSGKV PHFALFSIWR PLKTVHRDPL
ALSSAASFPV SDYVPCDQRE PTDHSIPSHL YRIVGRDDEN IDKNDDTYQT QSYLAYAPRD
AEKTSHAWHY ISEQQPSDVL VIQLFDNEME GHARAPLEGG KGKSDLGVGG AVHSAFELVD
QDEDAEARES IEVRVAAFW
