ID   CHS1_CASGL              Reviewed;         389 AA.
AC   Q9ZRR8;
DT   08-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Chalcone synthase;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase;
GN   Name=CHS1;
OS   Casuarina glauca (Swamp oak).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fagales; Casuarinaceae; Casuarina.
OX   NCBI_TaxID=3522;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Root nodule;
RX   PubMed=10482666; DOI=10.1104/pp.121.1.113;
RA   Laplaze L., Gherbi H., Frutz T., Pawlowski K., Franche C., Macheix J.J.,
RA   Auguy F., Bogusz D., Duhoux E.;
RT   "Flavan-containing cells delimit Frankia infected compartments in Casuarina
RT   glauca nodules.";
RL   Plant Physiol. 121:113-122(1999).
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; AJ132323; CAA10641.1; -; mRNA.
DR   AlphaFoldDB; Q9ZRR8; -.
DR   SMR; Q9ZRR8; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..389
FT                   /note="Chalcone synthase"
FT                   /id="PRO_0000215964"
FT   ACT_SITE        164
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ   SEQUENCE   389 AA;  42603 MW;  185BE6F345EFA7DD CRC64;
     MVTVEEVRKA QRAEGPATVL AIGTATPPNC LDQSTYPDYY FRITNSEHKT ELKEKFQRMC
     DKSMIKKRYM YLTEEILKEH PNMCAYMAPS LDARQDMVVV EIPKLGKEAA VKAIKEWGQP
     KSKITHLVFC TTSGVDMPGA DYQLTKLLGL RPSVKRLMMY QQGCFAGGTV LRLAKDLAEN
     NRGARVLVVC SEITAVTFRG PSDTHLDSLV GQALFGDGAA AIIVGADPLP EVEKPLFEVV
     STAQTILPDS DGAIDGHLRE VGVTFHLLKD VPGLISKNIE KSLVEAFQPL GISDWNSLFW
     IAHPGGPAIL DQVEEKLALK PEKLGATRHV LSEYGNMSSA CVLFILDEMR RKSAEKGLKT
     TGEGLDWGVL FGFGPGLTVE TVVLHSLTT