ID   CHS1_CICAR              Reviewed;         389 AA.
AC   Q9SML4;
DT   08-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=Chalcone synthase 1;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase 1;
GN   Name=CHS1;
OS   Cicer arietinum (Chickpea) (Garbanzo).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Cicereae; Cicer.
OX   NCBI_TaxID=3827;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. ILC 3279; TISSUE=Leaf;
RA   Hanselle T., Schwenger-Erger C., Barz W.;
RT   "Isolation of a full length chalcone synthase cDNA from infected chickpea
RT   plants (Cicer arietinum L.).";
RL   (er) Plant Gene Register PGR99-100(1999).
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; AJ012822; CAA10190.1; -; mRNA.
DR   AlphaFoldDB; Q9SML4; -.
DR   SMR; Q9SML4; -.
DR   UniPathway; UPA00154; -.
DR   Proteomes; UP000087171; Genome assembly.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Flavonoid biosynthesis; Reference proteome; Transferase.
FT   CHAIN           1..389
FT                   /note="Chalcone synthase 1"
FT                   /id="PRO_0000215967"
FT   ACT_SITE        164
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ   SEQUENCE   389 AA;  42874 MW;  04CB0D50086168AC CRC64;
     MVSVSEIRKA QRAEGPATIL AIGTANPSNR VEQSTYPDFY FKITNSEHKV ELKQKFQRMC
     DKSMIKSRYM YLTEEILKEN PSVCEYMAPS LDVRQDMVVV EVPRLGKEAA VKAIKEWGQP
     KSKITHLIFC TTSGVDMPGA DYQLTKLLGL RPYVKRYMMY QQGCFAGGTV LRLAKDLAEN
     NKGARVLVVC SEVTAVTFRG PSDTHLDSLV GQALFGDGAA ALIVGSDPIP EIEKPIFEMV
     WTAQTIAPDS EGAIDGHLRE AGLTFHLLKD VPGIVSKNID KALIEAFQPL NISDYNSIFW
     IAHPGGPAIL DQVEEKLALK PEKMRATREV LSEYGNMSSA CVLFILDEMR RKSAKDGLKT
     TGEGLEWGVL FGFGPGLTIE TVVLHSVAI