ID   CHS1_CITSI              Reviewed;         389 AA.
AC   Q9XJ58;
DT   08-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Chalcone synthase 1;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase 1;
GN   Name=CHS1;
OS   Citrus sinensis (Sweet orange) (Citrus aurantium var. sinensis).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Sapindales; Rutaceae; Aurantioideae; Citrus.
OX   NCBI_TaxID=2711;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=10483126; DOI=10.1093/oxfordjournals.pcp.a029589;
RA   Moriguchi T., Kita M., Tomono Y., Endo-Inagaki T., Omura M.;
RT   "One type of chalcone synthase gene expressed during embryogenesis
RT   regulates the flavonoid accumulation in citrus cell cultures.";
RL   Plant Cell Physiol. 40:651-655(1999).
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; AB009350; BAA81663.1; -; mRNA.
DR   AlphaFoldDB; Q9XJ58; -.
DR   SMR; Q9XJ58; -.
DR   STRING; 2711.XP_006489796.1; -.
DR   eggNOG; ENOG502QRSY; Eukaryota.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..389
FT                   /note="Chalcone synthase 1"
FT                   /id="PRO_0000215968"
FT   ACT_SITE        163
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ   SEQUENCE   389 AA;  42547 MW;  4A066BAA5F28DE8F CRC64;
     MVTVDEVRKA QRAQGPATIM AIGTATPPNC VDQSTYPDYY FRITNSEHMT DLKEKFKRMC
     DKSMIKKRYM YLTEEILKEN PNVCAYMAPS LDTRQDMVVV EVPRLGKAAT EAIKEWGQPK
     SKITHLVFCT TSGVDMPGAD YRLTKLLGLR PSVKRLMMYQ QGCFAGGTVL RLAKDLAENN
     KGARVLVVCS EITAVTFRGP SDTHLDSLVG QALFGDGAAA IIIGADPIPE IEKPMFELVS
     TAQTILPDSD GSIDGHLREA GLTFHLLKDV PGLISKNIQK SLTEAFKPLG ISDWNSIFWI
     AHPGGPAILD QVEEKLGLKP EKLRATRHVL SEYGNMSSAC VLFILDEMRK KSAEDGLETA
     GEGLEWGVLF GFGPGLTVET VVLHSVAAA