ID   CHS1_DAUCA              Reviewed;         389 AA.
AC   Q9ZS41;
DT   08-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=Chalcone synthase 1;
DE            EC=2.3.1.74;
DE   AltName: Full=DcCHS1;
DE   AltName: Full=Naringenin-chalcone synthase 1;
GN   Name=CHS1;
OS   Daucus carota (Wild carrot).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; campanulids; Apiales; Apiaceae; Apioideae; Scandiceae; Daucinae;
OC   Daucus; Daucus sect. Daucus.
OX   NCBI_TaxID=4039;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=10872233; DOI=10.1007/s004250050708;
RA   Hirner A.A., Seitz H.U.;
RT   "Isoforms of chalcone synthase in Daucus carota L. and their differential
RT   expression in organs from the European wild carrot and in ultraviolet-A-
RT   irradiated cell cultures.";
RL   Planta 210:993-998(2000).
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; AJ006779; CAA07244.1; -; mRNA.
DR   AlphaFoldDB; Q9ZS41; -.
DR   SMR; Q9ZS41; -.
DR   EnsemblPlants; KZM83217; KZM83217; DCAR_030786.
DR   Gramene; KZM83217; KZM83217; DCAR_030786.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..389
FT                   /note="Chalcone synthase 1"
FT                   /id="PRO_0000215970"
FT   ACT_SITE        164
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ   SEQUENCE   389 AA;  42707 MW;  CDBD13EF4D6D53B9 CRC64;
     MVTVNEFRKA QRAEGPATVL AIGTATPPNC VDQSAYADYY FRITNSEDKP ELKEKFRRMC
     EKSMINTRYM HLTEDLLKQN PSFCEYMASS LDARQDIVVN EVPKLGKEAA LRAIKEWGQP
     KSKITHLIFC TTSGVDMPGA DFRLTKLLGL RPSVKRFMMY QQGCFAGGTV LRLAKDLAEN
     NKNARVLVVC SEITVITFRG PNDTHLDSLV GQALFGDGAG AVIVGSDPVI GIEKPLFEIV
     SAAQTILPDS DGAIDGHLRE VGLTFHLLKD VPGLISKNIR KSLVEAFKPL GISDWNSIFW
     IAHPGGPAIL DQVETELSLK PEKLKSTRQV LRDYGNMSSA CVLFILDEMR KASAKDGHRT
     TGEGLDWGVL FGFGPGLTVE TVVLHSVPP