ID   CHS1_GERHY              Reviewed;         398 AA.
AC   P48390;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   25-MAY-2022, entry version 87.
DE   RecName: Full=Chalcone synthase 1;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase 1;
GN   Name=CHS1;
OS   Gerbera hybrida (Daisy).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; campanulids; Asterales; Asteraceae; Mutisioideae; Mutisieae;
OC   Gerbera.
OX   NCBI_TaxID=18101;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Corolla;
RX   PubMed=7787187; DOI=10.1007/bf00042037;
RA   Helariutta Y., Elomaa P., Kotilainen M., Griesbach R.J., Schroeder J.,
RA   Teeri T.H.;
RT   "Chalcone synthase-like genes active during corolla development are
RT   differentially expressed and encode enzymes with different catalytic
RT   properties in Gerbera hybrida (Asteraceae).";
RL   Plant Mol. Biol. 28:47-60(1995).
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; Z38096; CAA86218.1; -; mRNA.
DR   PIR; S56699; S56699.
DR   AlphaFoldDB; P48390; -.
DR   SMR; P48390; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..398
FT                   /note="Chalcone synthase 1"
FT                   /id="PRO_0000215977"
FT   ACT_SITE        167
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ   SEQUENCE   398 AA;  43494 MW;  A5D095314D6F9A1D CRC64;
     MASSVDMKAI RDAQRAEGPA TILAIGTATP ANCVYQADYP DYYFRITKSE HMVDLKEKFK
     RMCDKSMIRK RYMHITEEYL KQNPNMCAYM APSLDVRQDL VVVEVPKLGK EAAMKAIKEW
     GHPKSKITHL IFCTTSGVDM PGADYQLTKL LGLRPSVKRF MMYQQGCFAG GTVLRLAKDL
     AENNKGARVL VVCSEITAVT FRGPNDTHLD SLVGQALFGD GAAAVIVGSD PDLTTERPLF
     EMVSAAQTIL PDSEGAIDGH LREVGLTFHL LKDVPGLISK NIEKALTTAF SPLGINDWNS
     IFWIAHPGGP AILDQVELKL GLKEEKLRAT RHVLSEYGNM SSACVLFIID EMRKKSSENG
     AGTTGEGLEW GVLFGFGPGL TVETVVLHSV PTTVTVAV