ID   CHS1_HORVU              Reviewed;         398 AA.
AC   P26018;
DT   01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1992, sequence version 1.
DT   25-MAY-2022, entry version 93.
DE   RecName: Full=Chalcone synthase 1;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase 1;
GN   Name=CHS1; Synonyms=CHS;
OS   Hordeum vulgare (Barley).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX   NCBI_TaxID=4513;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. C2; TISSUE=Leaf;
RX   PubMed=1863766; DOI=10.1007/bf00016087;
RA   Rohde W., Doerr S., Salamini F., Becker D.;
RT   "Structure of a chalcone synthase gene from Hordeum vulgare.";
RL   Plant Mol. Biol. 16:1103-1106(1991).
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; X58339; CAA41250.1; -; Genomic_DNA.
DR   PIR; S16275; S16275.
DR   AlphaFoldDB; P26018; -.
DR   SMR; P26018; -.
DR   UniPathway; UPA00154; -.
DR   ExpressionAtlas; P26018; baseline and differential.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..398
FT                   /note="Chalcone synthase 1"
FT                   /id="PRO_0000215979"
FT   ACT_SITE        167
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ   SEQUENCE   398 AA;  43532 MW;  01E7AC1656FCA70D CRC64;
     MAATMTVEEV RNAQRAEGPA TVLAIGTATP ANCVYQADYP DYYFKITKSD HMADLKEKFK
     RMCDKSQIRK RYMHLTEEIL EENPNMCAYM APSLDARQDI VVVEVPKLGK AAAQKAIKEW
     GQPRSKITHL VFCTTSGVDM PGADYQLTKM LGLRPSVKRL MMYQQGCFAG GTVLRLAKDL
     AENNRGARVL VVCSEITAVT FRGPHESHLD SLVGQALFGD GAAAVIIGAD PDLSVERPLF
     QLVSASQTIL PDSEGAIDGH LREVGLTFHL LKDVPGLISK NIERALEEAF KPLGIDHWNS
     VFWIAHQGGP AILDMVEAKV NLNKERMRAT RHVLSEYGNM SSACVLFIMD EMRKRSAEDG
     HATTGEGMDW GVLFGFGPGL TVETVVLHSV PISAGATA