ID   CHS1_MEDSA              Reviewed;         389 AA.
AC   P30073;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1993, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Chalcone synthase 1;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase 1;
GN   Name=CHS1;
OS   Medicago sativa (Alfalfa).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX   NCBI_TaxID=3879;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=8507827; DOI=10.1007/bf00014932;
RA   Junghans H., Dalkin K., Dixon R.A.;
RT   "Stress responses in alfalfa (Medicago sativa L.). 15. Characterization and
RT   expression patterns of members of a subset of the chalcone synthase
RT   multigene family.";
RL   Plant Mol. Biol. 22:239-253(1993).
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; L02901; AAA02823.1; -; mRNA.
DR   PIR; S35163; S35163.
DR   AlphaFoldDB; P30073; -.
DR   SMR; P30073; -.
DR   PRIDE; P30073; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..389
FT                   /note="Chalcone synthase 1"
FT                   /id="PRO_0000216006"
FT   ACT_SITE        164
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ   SEQUENCE   389 AA;  42767 MW;  8C13260B8B730F5A CRC64;
     MVSVAEIRQA QRAEGPATIM AIGTANPANC VEQSTYPDFY FKITNSEHKV ELKEKFQRMC
     DKSMIKRRYM YLTEEILKDN PRVCEYMAPS LAARQDMAVV VVPRLGKEAA VKAIKEWGQP
     KSKITHLIFC TTSGVDMPGA DYQLTKLLGL RPYVKRYMMY QQGCFAGGTV LRLAKDLAEN
     NKGARVLVVC SEETPVTFRG PSDTHLDSLV GQALFGDGAA ALIVGSDPIP EIEKPIFEMV
     WTAHTIAPDS EGAIDGHLRE AGLTFHLLKD VPGIVSKNID KALIEAFQPL NISDYNSIFW
     IAHPGGPAIL DQVEEKLGLK PEKMKATREV LSEYGNMSSA CVLFILDEMR KKSVQAGLKT
     TGEGLDWGVL FGFGPGLTIE TVVLHSVAI