CHS1_ORYSI
ID CHS1_ORYSI Reviewed; 398 AA.
AC A2ZEX7; P48405; P93710; Q9AVC2;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 20-MAR-2007, sequence version 1.
DT 03-AUG-2022, entry version 77.
DE RecName: Full=Chalcone synthase 1;
DE Short=OsCHS1;
DE EC=2.3.1.74;
DE AltName: Full=Naregenin-chalcone synthase;
GN Name=CHS1; Synonyms=CHS; ORFNames=OsI_035120;
OS Oryza sativa subsp. indica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39946;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Purple Puttu; TISSUE=Leaf;
RA Scheffler B.E., Reddy A.R., Hoffmann I., Wienand U.;
RT "Chalcone synthase cDNA from Oryza sativa.";
RL (er) Plant Gene Register PGR95-071(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. 93-11;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
CC -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC which can under specific conditions spontaneously isomerize into
CC naringenin.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC synthases family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X89859; CAA61955.1; -; mRNA.
DR EMBL; CM000136; EAY81161.1; -; Genomic_DNA.
DR PIR; S58190; S58190.
DR PDB; 4YJY; X-ray; 1.86 A; A/B=1-398.
DR PDBsum; 4YJY; -.
DR AlphaFoldDB; A2ZEX7; -.
DR SMR; A2ZEX7; -.
DR STRING; 39946.A2ZEX7; -.
DR EnsemblPlants; BGIOSGA033874-TA; BGIOSGA033874-PA; BGIOSGA033874.
DR Gramene; BGIOSGA033874-TA; BGIOSGA033874-PA; BGIOSGA033874.
DR HOGENOM; CLU_034992_2_0_1; -.
DR OMA; NFMGCYA; -.
DR UniPathway; UPA00154; -.
DR Proteomes; UP000007015; Chromosome 11.
DR GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.47.10; -; 2.
DR InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR InterPro; IPR011141; Polyketide_synthase_type-III.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR11877; PTHR11877; 1.
DR Pfam; PF02797; Chal_sti_synt_C; 1.
DR Pfam; PF00195; Chal_sti_synt_N; 1.
DR PIRSF; PIRSF000451; PKS_III; 1.
DR SUPFAM; SSF53901; SSF53901; 2.
DR PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acyltransferase; Flavonoid biosynthesis; Reference proteome;
KW Transferase.
FT CHAIN 1..398
FT /note="Chalcone synthase 1"
FT /id="PRO_0000293085"
FT ACT_SITE 167
FT /note="Acyl-thioester intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
FT BINDING 58..65
FT /ligand="CoA"
FT /ligand_id="ChEBI:CHEBI:57287"
FT /evidence="ECO:0000250"
FT BINDING 200
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 219..220
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 311
FT /ligand="CoA"
FT /ligand_id="ChEBI:CHEBI:57287"
FT /evidence="ECO:0000250"
FT HELIX 7..14
FT /evidence="ECO:0007829|PDB:4YJY"
FT STRAND 21..28
FT /evidence="ECO:0007829|PDB:4YJY"
FT STRAND 33..35
FT /evidence="ECO:0007829|PDB:4YJY"
FT HELIX 36..38
FT /evidence="ECO:0007829|PDB:4YJY"
FT HELIX 39..46
FT /evidence="ECO:0007829|PDB:4YJY"
FT HELIX 53..65
FT /evidence="ECO:0007829|PDB:4YJY"
FT STRAND 70..72
FT /evidence="ECO:0007829|PDB:4YJY"
FT HELIX 77..82
FT /evidence="ECO:0007829|PDB:4YJY"
FT HELIX 84..86
FT /evidence="ECO:0007829|PDB:4YJY"
FT STRAND 88..90
FT /evidence="ECO:0007829|PDB:4YJY"
FT HELIX 94..120
FT /evidence="ECO:0007829|PDB:4YJY"
FT HELIX 124..126
FT /evidence="ECO:0007829|PDB:4YJY"
FT STRAND 129..136
FT /evidence="ECO:0007829|PDB:4YJY"
FT HELIX 143..151
FT /evidence="ECO:0007829|PDB:4YJY"
FT STRAND 158..164
FT /evidence="ECO:0007829|PDB:4YJY"
FT HELIX 169..182
FT /evidence="ECO:0007829|PDB:4YJY"
FT STRAND 188..195
FT /evidence="ECO:0007829|PDB:4YJY"
FT HELIX 197..199
FT /evidence="ECO:0007829|PDB:4YJY"
FT HELIX 209..217
FT /evidence="ECO:0007829|PDB:4YJY"
FT STRAND 221..230
FT /evidence="ECO:0007829|PDB:4YJY"
FT TURN 233..235
FT /evidence="ECO:0007829|PDB:4YJY"
FT STRAND 240..249
FT /evidence="ECO:0007829|PDB:4YJY"
FT STRAND 256..262
FT /evidence="ECO:0007829|PDB:4YJY"
FT STRAND 265..270
FT /evidence="ECO:0007829|PDB:4YJY"
FT HELIX 274..290
FT /evidence="ECO:0007829|PDB:4YJY"
FT HELIX 291..293
FT /evidence="ECO:0007829|PDB:4YJY"
FT HELIX 298..300
FT /evidence="ECO:0007829|PDB:4YJY"
FT STRAND 301..305
FT /evidence="ECO:0007829|PDB:4YJY"
FT HELIX 310..320
FT /evidence="ECO:0007829|PDB:4YJY"
FT HELIX 324..327
FT /evidence="ECO:0007829|PDB:4YJY"
FT HELIX 328..337
FT /evidence="ECO:0007829|PDB:4YJY"
FT HELIX 341..343
FT /evidence="ECO:0007829|PDB:4YJY"
FT HELIX 344..358
FT /evidence="ECO:0007829|PDB:4YJY"
FT TURN 364..367
FT /evidence="ECO:0007829|PDB:4YJY"
FT STRAND 369..377
FT /evidence="ECO:0007829|PDB:4YJY"
FT TURN 378..380
FT /evidence="ECO:0007829|PDB:4YJY"
FT STRAND 381..389
FT /evidence="ECO:0007829|PDB:4YJY"
SQ SEQUENCE 398 AA; 43238 MW; 583FC50C65BB9755 CRC64;
MAAAVTVEEV RRAQRAEGPA TVLAIGTATP ANCVYQADYP DYYFRITKSE HMVELKEKFK
RMCDKSQIRK RYMHLTEEIL QENPNMCAYM APSLDARQDI VVVEVPKLGK AAAQKAIKEW
GQPRSRITHL VFCTTSGVDM PGADYQLAKM LGLRPNVSRL MMYQQGCFAG GTVLRVAKDL
AENNRGARVL AVCSEITAVT FRGPSESHLD SMVGQALFGD GAAAVIVGSD PDEAVERPLF
QMVSASQTIL PDSEGAIDGH LREVGLTFHL LKDVPGLISK NIERALGDAF TPLGISDWNS
IFWVAHPGGP AILDQVEAKV GLDKERMRAT RHVLSEYGNM SSACVLFILD EMRKRSAEDG
HATTGEGMDW GVLFGFGPGL TVETVVLHSV PITAGAAA