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CHS1_ORYSI
ID   CHS1_ORYSI              Reviewed;         398 AA.
AC   A2ZEX7; P48405; P93710; Q9AVC2;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   20-MAR-2007, sequence version 1.
DT   03-AUG-2022, entry version 77.
DE   RecName: Full=Chalcone synthase 1;
DE            Short=OsCHS1;
DE            EC=2.3.1.74;
DE   AltName: Full=Naregenin-chalcone synthase;
GN   Name=CHS1; Synonyms=CHS; ORFNames=OsI_035120;
OS   Oryza sativa subsp. indica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39946;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Purple Puttu; TISSUE=Leaf;
RA   Scheffler B.E., Reddy A.R., Hoffmann I., Wienand U.;
RT   "Chalcone synthase cDNA from Oryza sativa.";
RL   (er) Plant Gene Register PGR95-071(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. 93-11;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; X89859; CAA61955.1; -; mRNA.
DR   EMBL; CM000136; EAY81161.1; -; Genomic_DNA.
DR   PIR; S58190; S58190.
DR   PDB; 4YJY; X-ray; 1.86 A; A/B=1-398.
DR   PDBsum; 4YJY; -.
DR   AlphaFoldDB; A2ZEX7; -.
DR   SMR; A2ZEX7; -.
DR   STRING; 39946.A2ZEX7; -.
DR   EnsemblPlants; BGIOSGA033874-TA; BGIOSGA033874-PA; BGIOSGA033874.
DR   Gramene; BGIOSGA033874-TA; BGIOSGA033874-PA; BGIOSGA033874.
DR   HOGENOM; CLU_034992_2_0_1; -.
DR   OMA; NFMGCYA; -.
DR   UniPathway; UPA00154; -.
DR   Proteomes; UP000007015; Chromosome 11.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Acyltransferase; Flavonoid biosynthesis; Reference proteome;
KW   Transferase.
FT   CHAIN           1..398
FT                   /note="Chalcone synthase 1"
FT                   /id="PRO_0000293085"
FT   ACT_SITE        167
FT                   /note="Acyl-thioester intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
FT   BINDING         58..65
FT                   /ligand="CoA"
FT                   /ligand_id="ChEBI:CHEBI:57287"
FT                   /evidence="ECO:0000250"
FT   BINDING         200
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         219..220
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         311
FT                   /ligand="CoA"
FT                   /ligand_id="ChEBI:CHEBI:57287"
FT                   /evidence="ECO:0000250"
FT   HELIX           7..14
FT                   /evidence="ECO:0007829|PDB:4YJY"
FT   STRAND          21..28
FT                   /evidence="ECO:0007829|PDB:4YJY"
FT   STRAND          33..35
FT                   /evidence="ECO:0007829|PDB:4YJY"
FT   HELIX           36..38
FT                   /evidence="ECO:0007829|PDB:4YJY"
FT   HELIX           39..46
FT                   /evidence="ECO:0007829|PDB:4YJY"
FT   HELIX           53..65
FT                   /evidence="ECO:0007829|PDB:4YJY"
FT   STRAND          70..72
FT                   /evidence="ECO:0007829|PDB:4YJY"
FT   HELIX           77..82
FT                   /evidence="ECO:0007829|PDB:4YJY"
FT   HELIX           84..86
FT                   /evidence="ECO:0007829|PDB:4YJY"
FT   STRAND          88..90
FT                   /evidence="ECO:0007829|PDB:4YJY"
FT   HELIX           94..120
FT                   /evidence="ECO:0007829|PDB:4YJY"
FT   HELIX           124..126
FT                   /evidence="ECO:0007829|PDB:4YJY"
FT   STRAND          129..136
FT                   /evidence="ECO:0007829|PDB:4YJY"
FT   HELIX           143..151
FT                   /evidence="ECO:0007829|PDB:4YJY"
FT   STRAND          158..164
FT                   /evidence="ECO:0007829|PDB:4YJY"
FT   HELIX           169..182
FT                   /evidence="ECO:0007829|PDB:4YJY"
FT   STRAND          188..195
FT                   /evidence="ECO:0007829|PDB:4YJY"
FT   HELIX           197..199
FT                   /evidence="ECO:0007829|PDB:4YJY"
FT   HELIX           209..217
FT                   /evidence="ECO:0007829|PDB:4YJY"
FT   STRAND          221..230
FT                   /evidence="ECO:0007829|PDB:4YJY"
FT   TURN            233..235
FT                   /evidence="ECO:0007829|PDB:4YJY"
FT   STRAND          240..249
FT                   /evidence="ECO:0007829|PDB:4YJY"
FT   STRAND          256..262
FT                   /evidence="ECO:0007829|PDB:4YJY"
FT   STRAND          265..270
FT                   /evidence="ECO:0007829|PDB:4YJY"
FT   HELIX           274..290
FT                   /evidence="ECO:0007829|PDB:4YJY"
FT   HELIX           291..293
FT                   /evidence="ECO:0007829|PDB:4YJY"
FT   HELIX           298..300
FT                   /evidence="ECO:0007829|PDB:4YJY"
FT   STRAND          301..305
FT                   /evidence="ECO:0007829|PDB:4YJY"
FT   HELIX           310..320
FT                   /evidence="ECO:0007829|PDB:4YJY"
FT   HELIX           324..327
FT                   /evidence="ECO:0007829|PDB:4YJY"
FT   HELIX           328..337
FT                   /evidence="ECO:0007829|PDB:4YJY"
FT   HELIX           341..343
FT                   /evidence="ECO:0007829|PDB:4YJY"
FT   HELIX           344..358
FT                   /evidence="ECO:0007829|PDB:4YJY"
FT   TURN            364..367
FT                   /evidence="ECO:0007829|PDB:4YJY"
FT   STRAND          369..377
FT                   /evidence="ECO:0007829|PDB:4YJY"
FT   TURN            378..380
FT                   /evidence="ECO:0007829|PDB:4YJY"
FT   STRAND          381..389
FT                   /evidence="ECO:0007829|PDB:4YJY"
SQ   SEQUENCE   398 AA;  43238 MW;  583FC50C65BB9755 CRC64;
     MAAAVTVEEV RRAQRAEGPA TVLAIGTATP ANCVYQADYP DYYFRITKSE HMVELKEKFK
     RMCDKSQIRK RYMHLTEEIL QENPNMCAYM APSLDARQDI VVVEVPKLGK AAAQKAIKEW
     GQPRSRITHL VFCTTSGVDM PGADYQLAKM LGLRPNVSRL MMYQQGCFAG GTVLRVAKDL
     AENNRGARVL AVCSEITAVT FRGPSESHLD SMVGQALFGD GAAAVIVGSD PDEAVERPLF
     QMVSASQTIL PDSEGAIDGH LREVGLTFHL LKDVPGLISK NIERALGDAF TPLGISDWNS
     IFWVAHPGGP AILDQVEAKV GLDKERMRAT RHVLSEYGNM SSACVLFILD EMRKRSAEDG
     HATTGEGMDW GVLFGFGPGL TVETVVLHSV PITAGAAA
 
 
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