CHS1_ORYSJ
ID CHS1_ORYSJ Reviewed; 398 AA.
AC Q2R3A1; A0A0P0Y338; P48405; P93710; Q9AVC2;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 24-JAN-2006, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Chalcone synthase 1;
DE Short=OsCHS1;
DE EC=2.3.1.74;
DE AltName: Full=Naregenin-chalcone synthase;
GN Name=CHS1; Synonyms=CHS; OrderedLocusNames=Os11g0530600, LOC_Os11g32650;
GN ORFNames=OsJ_032788;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Nipponbare; TISSUE=Leaf;
RA Minami E., He D., Minami N., Shibuya N.;
RT "Sequence analysis and some characterization of single-copied chalcone
RT synthase from rice.";
RL Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RA Ichikawa H., Okano E., Shimizu T., Nakamura S., Kawasaki S., Minami E.;
RT "Molecular cloning and characterization of chalcone synthase (CHS) gene
RT from rice (Oryza sativa L).";
RL Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16188032; DOI=10.1186/1741-7007-3-20;
RG The rice chromosomes 11 and 12 sequencing consortia;
RT "The sequence of rice chromosomes 11 and 12, rich in disease resistance
RT genes and recent gene duplications.";
RL BMC Biol. 3:20-20(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [6]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
CC -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC which can under specific conditions spontaneously isomerize into
CC naringenin.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC synthases family. {ECO:0000305}.
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DR EMBL; AB000801; BAA19186.2; -; mRNA.
DR EMBL; AB058397; BAB39764.1; -; Genomic_DNA.
DR EMBL; DP000010; ABA94123.1; -; Genomic_DNA.
DR EMBL; AP008217; BAF28372.1; -; Genomic_DNA.
DR EMBL; AP014967; BAT14279.1; -; Genomic_DNA.
DR EMBL; CM000148; EAZ18579.1; -; Genomic_DNA.
DR RefSeq; XP_015618054.1; XM_015762568.1.
DR AlphaFoldDB; Q2R3A1; -.
DR SMR; Q2R3A1; -.
DR STRING; 4530.OS11T0530600-01; -.
DR PaxDb; Q2R3A1; -.
DR PRIDE; Q2R3A1; -.
DR EnsemblPlants; Os11t0530600-01; Os11t0530600-01; Os11g0530600.
DR GeneID; 4350636; -.
DR Gramene; Os11t0530600-01; Os11t0530600-01; Os11g0530600.
DR KEGG; osa:4350636; -.
DR eggNOG; ENOG502QRSY; Eukaryota.
DR HOGENOM; CLU_034992_2_0_1; -.
DR InParanoid; Q2R3A1; -.
DR OMA; NFMGCYA; -.
DR OrthoDB; 950070at2759; -.
DR PlantReactome; R-OSA-1119531; Flavonoid biosynthesis.
DR UniPathway; UPA00154; -.
DR Proteomes; UP000000763; Chromosome 11.
DR Proteomes; UP000007752; Chromosome 11.
DR Proteomes; UP000059680; Chromosome 11.
DR Genevisible; Q2R3A1; OS.
DR GO; GO:0016747; F:acyltransferase activity, transferring groups other than amino-acyl groups; IBA:GO_Central.
DR GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0030639; P:polyketide biosynthetic process; IBA:GO_Central.
DR Gene3D; 3.40.47.10; -; 2.
DR InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR InterPro; IPR011141; Polyketide_synthase_type-III.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR11877; PTHR11877; 1.
DR Pfam; PF02797; Chal_sti_synt_C; 1.
DR Pfam; PF00195; Chal_sti_synt_N; 1.
DR PIRSF; PIRSF000451; PKS_III; 1.
DR SUPFAM; SSF53901; SSF53901; 2.
DR PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE 2: Evidence at transcript level;
KW Acyltransferase; Flavonoid biosynthesis; Reference proteome; Transferase.
FT CHAIN 1..398
FT /note="Chalcone synthase 1"
FT /id="PRO_0000216017"
FT ACT_SITE 167
FT /note="Acyl-thioester intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
FT BINDING 58..65
FT /ligand="CoA"
FT /ligand_id="ChEBI:CHEBI:57287"
FT /evidence="ECO:0000250"
FT BINDING 200
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 219..220
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 311
FT /ligand="CoA"
FT /ligand_id="ChEBI:CHEBI:57287"
FT /evidence="ECO:0000250"
SQ SEQUENCE 398 AA; 43265 MW; FD5D8C28FE60FAE3 CRC64;
MAAAVTVEEV RRAQRAEGPA TVLAIGTATP ANCVYQADYP DYYFRITKSE HMVELKEKFK
RMCDKSQIRK RYMHLTEEIL QENPNMCAYM APSLDARQDI VVVEVPKLGK AAAQKAIKEW
GQPRSRITHL VFCTTSGVDM PGADYQLAKM LGLRPNVNRL MMYQQGCFAG GTVLRVAKDL
AENNRGARVL AVCSEITAVT FRGPSESHLD SMVGQALFGD GAAAVIVGSD PDEAVERPLF
QMVSASQTIL PDSEGAIDGH LREVGLTFHL LKDVPGLISK NIERALGDAF TPLGISDWNS
IFWVAHPGGP AILDQVEAKV GLDKERMRAT RHVLSEYGNM SSACVLFILD EMRKRSAEDG
HATTGEGMDW GVLFGFGPGL TVETVVLHSV PITAGAAA
