ID   CHS1_SECCE              Reviewed;         392 AA.
AC   P53414;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=Chalcone synthase 1;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase 1;
GN   Name=CHS1;
OS   Secale cereale (Rye).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Hordeinae; Secale.
OX   NCBI_TaxID=4550;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Kustro;
RA   Haussuehl K.K., Rohde W., Weissenboeck G.;
RT   "Expression of chalcone synthase genes in coleoptiles and primary leaves of
RT   Secale cereale L. after induction by UV radiation: evidence for a UV-
RT   protective role of the coleoptile.";
RL   Bot. Acta 109:229-238(1996).
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; X92548; CAA63306.1; -; Genomic_DNA.
DR   AlphaFoldDB; P53414; -.
DR   SMR; P53414; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..392
FT                   /note="Chalcone synthase 1"
FT                   /id="PRO_0000216048"
FT   ACT_SITE        167
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ   SEQUENCE   392 AA;  43006 MW;  B543EDAE67A861E6 CRC64;
     MAATMTVEEV RKAQRAEGPA TVLAIGTATP ANCVYQADYP DYYFKITKSD HMADLKEKFK
     RMCDKSQIRK RYMHLTEEIL QDNPNMCAYM APSLDARQDI VVVEVPKLGK AAAQKAIKEW
     GQPRSKITHL VFCTTSGVDM PGADYQLTKM LGLRPSVKRL MMYQQGCFAG GTVLRLAKDL
     AENNRGARVL VVCSEITAVT FRGPHEFDSL VGQALFGDGA AAVIVGADPD ESVERPLFQL
     VSASQTILPD SEGAIDGHLR EVGLTFHLLK DVPGLISKNI ERALEDAFKP LGIDDWNSVF
     WIAHPGGPAI LDMVEAKVNL NKERMRATRH VLSEYGNMSS ACVLFIMDEM RKRSAEDGHT
     TTGEGMDWGV LFGFGPGLTV ETVVLHSVPV TA