ID   CHS2_CITSI              Reviewed;         391 AA.
AC   Q9XJ57;
DT   08-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=Chalcone synthase 2;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase 2;
GN   Name=CHS2;
OS   Citrus sinensis (Sweet orange) (Citrus aurantium var. sinensis).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Sapindales; Rutaceae; Aurantioideae; Citrus.
OX   NCBI_TaxID=2711;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=10483126; DOI=10.1093/oxfordjournals.pcp.a029589;
RA   Moriguchi T., Kita M., Tomono Y., Endo-Inagaki T., Omura M.;
RT   "One type of chalcone synthase gene expressed during embryogenesis
RT   regulates the flavonoid accumulation in citrus cell cultures.";
RL   Plant Cell Physiol. 40:651-655(1999).
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; AB009351; BAA81664.1; -; mRNA.
DR   RefSeq; NP_001306986.1; NM_001320057.1.
DR   AlphaFoldDB; Q9XJ57; -.
DR   SMR; Q9XJ57; -.
DR   STRING; 2711.XP_006468926.1; -.
DR   GeneID; 102607309; -.
DR   KEGG; cit:102607309; -.
DR   eggNOG; ENOG502QRSY; Eukaryota.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..391
FT                   /note="Chalcone synthase 2"
FT                   /id="PRO_0000215969"
FT   ACT_SITE        164
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ   SEQUENCE   391 AA;  42592 MW;  F59029AC36890C54 CRC64;
     MATVQEIRNA QRADGPATVL AIGTATPAHS VNQADYPDYY FRITKSEHMT ELKEKFKRMC
     DKSMIKKRYM YLTEEILKEN PNMCAYMAPS LDARQDIVVV EVPKLGKEAA TKAIKEWGQP
     KSKITHLIFC TTSGVDMPGA DYQLTKLIGL RPSVKRFMMY QQGCFAGGTV LRLAKDLAEN
     NKGARVLVVC SEITAVTFRG PADTHLDSLV GQALFGDGAA AVIVGADPDT SVERPLYQLV
     STSQTILPDS DGAIDGHLRE VGLTFHLLKD VPGLISKNIE KSLSEAFAPL GISDWNSIFW
     IAHPGGPAIL DQVESKLGLK GEKLKATRQV LSEYGNMSSA CVLFILDEMR KKSVEEAKAT
     TGEGLDWGVL FGFGPGLTVE TVVLHSVPIK A