ID   CHS2_DAUCA              Reviewed;         397 AA.
AC   Q9ZS40;
DT   08-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Chalcone synthase 2;
DE            EC=2.3.1.74;
DE   AltName: Full=DcCHS2;
DE   AltName: Full=Naringenin-chalcone synthase 2;
GN   Name=CHS2;
OS   Daucus carota (Wild carrot).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; campanulids; Apiales; Apiaceae; Apioideae; Scandiceae; Daucinae;
OC   Daucus; Daucus sect. Daucus.
OX   NCBI_TaxID=4039;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=10872233; DOI=10.1007/s004250050708;
RA   Hirner A.A., Seitz H.U.;
RT   "Isoforms of chalcone synthase in Daucus carota L. and their differential
RT   expression in organs from the European wild carrot and in ultraviolet-A-
RT   irradiated cell cultures.";
RL   Planta 210:993-998(2000).
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; AJ006780; CAA07245.1; -; mRNA.
DR   AlphaFoldDB; Q9ZS40; -.
DR   SMR; Q9ZS40; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..397
FT                   /note="Chalcone synthase 2"
FT                   /id="PRO_0000215971"
FT   ACT_SITE        168
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ   SEQUENCE   397 AA;  43559 MW;  9386F44B9132EDEE CRC64;
     MANHNAEIEE IRKRQRAQGP ANILAIGTAT PSNCVYQADY PDYYFRITNS EHMSDLKLKF
     KRMCEKSMIR KRYMHITEEY LKENPNVCAY EAPSLDARQD LVVVEVPRLG KEAAAKAIKE
     WGHPKSKITH LIFCTTSGVD MPGADYQLTK LLGLRPSVKR FMMYQQGCFA GGTVLRLAKD
     LAENNTGARV LVVCSEITAV TFRGPSDSHL DSLVGQALFG DGAAAVIVGS DPDLSVERPL
     FQLISAAQTI LPDSDGAIDG HLREVGLTFH LLKDVPGLIS KNIEKSLKEA FGPIGISDWN
     SLFWIAHPGG PAILDQVELK LGLKEEKMRA TRQVLSDYGN MSSACVLFIL DEMRKKSIEE
     GKATTGDGLD WGVLFGFGPG LTVETVVLHS VPATITH