CHS2_ORYSJ
ID CHS2_ORYSJ Reviewed; 403 AA.
AC Q8H4L3; A0A0P0X3Z1;
DT 03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Chalcone synthase 2;
DE Short=OsCHS2;
DE EC=2.3.1.74;
DE AltName: Full=Naregenin-chalcone synthase;
GN Name=CHS2; OrderedLocusNames=Os07g0214900, LOC_Os07g11440;
GN ORFNames=OJ1116_C08.125;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
CC -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC which can under specific conditions spontaneously isomerize into
CC naringenin. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC synthases family. {ECO:0000305}.
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DR EMBL; AP004002; BAC20662.1; -; Genomic_DNA.
DR EMBL; AP008213; BAF21101.1; -; Genomic_DNA.
DR EMBL; AP014963; BAT00622.1; -; Genomic_DNA.
DR RefSeq; XP_015646206.1; XM_015790720.1.
DR AlphaFoldDB; Q8H4L3; -.
DR SMR; Q8H4L3; -.
DR STRING; 4530.OS07T0214900-00; -.
DR PaxDb; Q8H4L3; -.
DR PRIDE; Q8H4L3; -.
DR EnsemblPlants; Os07t0214900-00; Os07t0214900-00; Os07g0214900.
DR GeneID; 4342725; -.
DR Gramene; Os07t0214900-00; Os07t0214900-00; Os07g0214900.
DR KEGG; osa:4342725; -.
DR eggNOG; ENOG502QRSY; Eukaryota.
DR HOGENOM; CLU_034992_2_0_1; -.
DR InParanoid; Q8H4L3; -.
DR OMA; PCETHLD; -.
DR OrthoDB; 19161at2759; -.
DR PlantReactome; R-OSA-1119513; Pinobanksin biosynthesis.
DR PlantReactome; R-OSA-1119531; Flavonoid biosynthesis.
DR PlantReactome; R-OSA-1119630; Resveratrol biosynthesis.
DR UniPathway; UPA00154; -.
DR Proteomes; UP000000763; Chromosome 7.
DR Proteomes; UP000059680; Chromosome 7.
DR GO; GO:0016747; F:acyltransferase activity, transferring groups other than amino-acyl groups; IBA:GO_Central.
DR GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0030639; P:polyketide biosynthetic process; IBA:GO_Central.
DR Gene3D; 3.40.47.10; -; 2.
DR InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR InterPro; IPR011141; Polyketide_synthase_type-III.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR11877; PTHR11877; 1.
DR Pfam; PF02797; Chal_sti_synt_C; 1.
DR Pfam; PF00195; Chal_sti_synt_N; 1.
DR PIRSF; PIRSF000451; PKS_III; 1.
DR SUPFAM; SSF53901; SSF53901; 2.
DR PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE 3: Inferred from homology;
KW Acyltransferase; Flavonoid biosynthesis; Reference proteome; Transferase.
FT CHAIN 1..403
FT /note="Chalcone synthase 2"
FT /id="PRO_0000407327"
FT ACT_SITE 168
FT /note="Acyl-thioester intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
FT BINDING 59..66
FT /ligand="CoA"
FT /ligand_id="ChEBI:CHEBI:57287"
FT /evidence="ECO:0000250"
FT BINDING 220..221
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 313
FT /ligand="CoA"
FT /ligand_id="ChEBI:CHEBI:57287"
FT /evidence="ECO:0000250"
SQ SEQUENCE 403 AA; 43925 MW; 1E2622C4769F7B62 CRC64;
MVTSTVKLEE VRRMQRAEGM AAVLAIGTAT PANCVYQTDY PDYYFRVTNS EHLTNLKERF
QRMCESSQIR KRYTHLTEEI LQENPSMCVF TAPSLDARQD MVVAEVPKLG KAAAEEAIKE
WGQPMSRITH LVFCTTNGVD MPGADYQVAK MLGLPTSVKR LMMYQQGCFA GGTVLRVAKD
LAENNRGARV LVVCSEIMAM AFRGPSESHL DSLVGHALFG DGAAAVIVGS DPDEAADERP
LFQIVSASQT ILPGTEDAIV GHLREVGLTF HLPKDVPEFI SDSVEGALTD AFMPLGVHDW
NSIFWVVHPG GPAILDQVEE KVALHKARMR ASRNVLSEYG NMASATVLFV LDEMRKLSAD
DGHATTGEGM DWGVLFGFGP GLTVETIVLH SVPITAAAPL IMQ
