ID   CHS2_PEA                Reviewed;         389 AA.
AC   Q01287;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1993, sequence version 1.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=Chalcone synthase 2;
DE            EC=2.3.1.74;
DE   AltName: Full=Naregenin-chalcone synthase 2;
GN   Name=CHS2;
OS   Pisum sativum (Garden pea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Fabeae; Pisum.
OX   NCBI_TaxID=3888;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Midoriusui; TISSUE=Epicotyl;
RX   PubMed=1581561; DOI=10.1007/bf00019221;
RA   Ichinose Y., Kawamata S., Yamada T., An C., Kajiwara T., Shiraishi T.,
RA   Oku H.;
RT   "Molecular cloning of chalcone synthase cDNAs from Pisum sativum.";
RL   Plant Mol. Biol. 18:1009-1012(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-128.
RC   STRAIN=cv. Midoriusui; TISSUE=Epicotyl;
RX   PubMed=8467077; DOI=10.1007/bf00027112;
RA   An C., Ichinose Y., Yamada T., Tanaka Y., Shiraishi T., Oku H.;
RT   "Organization of the genes encoding chalcone synthase in Pisum sativum.";
RL   Plant Mol. Biol. 21:789-803(1993).
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; X63334; CAA44934.1; -; mRNA.
DR   EMBL; D10662; BAA01513.1; -; Genomic_DNA.
DR   PIR; S20932; S20932.
DR   AlphaFoldDB; Q01287; -.
DR   SMR; Q01287; -.
DR   PRIDE; Q01287; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..389
FT                   /note="Chalcone synthase 2"
FT                   /id="PRO_0000216019"
FT   ACT_SITE        164
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ   SEQUENCE   389 AA;  42845 MW;  0585EC37CA4F4072 CRC64;
     MVTVSEIRKA QRAEGPATIL AIGTANPANC VEQSTYPDFY FKITNSEHKT VLKEKFQRMC
     DKSMIKRRYM YLTEDILKEN PSLCEYMAPS LDARQDMVVV EVPRLGKEAA VKAIKEWGQP
     KSKITHLIFC TTSGVDMPGA DYQLTKLLGL RPYVKRYMMY QQGCFAGGTV FRLAKDLAEN
     NKNARVLVVC SEVTAVTFRG PSDTHLDSLV GQALFGDGAA ALIVGSDPVP EIEKPIFEMV
     WTAQTIAPDS EGAIDGHLRE AGLTFHLLKD VPGIVSKNID KALVEAFKPL GISDYNSIFW
     IAHPGGPAIL DQVEQKLALK PEKMRATREV LSEYGNMSSA CVLFILDEMR KKSTQDGLNT
     TGEGLEWGVL FGFGPGLTIE TVVLRSVAI