CHS2_SCHPO
ID CHS2_SCHPO Reviewed; 926 AA.
AC O74756; Q9US88;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 25-MAY-2022, entry version 141.
DE RecName: Full=Chitin synthase-like protein 2;
GN Name=chs2; ORFNames=SPBC1709.01, SPBC1734.17;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 720-925, AND SUBCELLULAR
RP LOCATION.
RC STRAIN=ATCC 38364 / 968;
RX PubMed=10759889; DOI=10.1046/j.1365-2443.2000.00317.x;
RA Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T.,
RA Hiraoka Y.;
RT "Large-scale screening of intracellular protein localization in living
RT fission yeast cells by the use of a GFP-fusion genomic DNA library.";
RL Genes Cells 5:169-190(2000).
RN [3]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=12914947; DOI=10.1016/s0014-5793(03)00812-3;
RA Martin-Garcia R., Duran A., Valdivieso M.H.;
RT "In Schizosaccharomyces pombe chs2p has no chitin synthase activity but is
RT related to septum formation.";
RL FEBS Lett. 549:176-180(2003).
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=15449309; DOI=10.1002/yea.1145;
RA Matsuo Y., Matsuura Y., Tanaka K., Matsuda H., Kawamukai M.;
RT "Chr4, a Schizosaccharomyces pombe homologue of the Saccharomyces
RT cerevisiae Chs4p/Skt5p protein, is related to septum formation and is
RT required for the proper localization of Chs2.";
RL Yeast 21:1005-1019(2004).
CC -!- FUNCTION: Plays a role in septum formation. Has no chitin synthase
CC activity. {ECO:0000269|PubMed:12914947}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000269|PubMed:10759889,
CC ECO:0000269|PubMed:12914947, ECO:0000269|PubMed:15449309}; Multi-pass
CC membrane protein {ECO:0000269|PubMed:10759889,
CC ECO:0000269|PubMed:12914947, ECO:0000269|PubMed:15449309}. Note=Septum.
CC -!- SIMILARITY: Belongs to the chitin synthase family. {ECO:0000305}.
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DR EMBL; CU329671; CAA21311.1; -; Genomic_DNA.
DR EMBL; AB027967; BAA87271.1; -; Genomic_DNA.
DR PIR; T39629; T39629.
DR PIR; T39664; T39664.
DR RefSeq; NP_595434.1; NM_001021342.2.
DR AlphaFoldDB; O74756; -.
DR BioGRID; 276709; 21.
DR STRING; 4896.SPBC1709.01.1; -.
DR CAZy; GT2; Glycosyltransferase Family 2.
DR PaxDb; O74756; -.
DR EnsemblFungi; SPBC1709.01.1; SPBC1709.01.1:pep; SPBC1709.01.
DR GeneID; 2540176; -.
DR KEGG; spo:SPBC1709.01; -.
DR PomBase; SPBC1709.01; chs2.
DR VEuPathDB; FungiDB:SPBC1709.01; -.
DR eggNOG; KOG2571; Eukaryota.
DR HOGENOM; CLU_004760_3_1_1; -.
DR InParanoid; O74756; -.
DR OMA; MYEFTST; -.
DR PhylomeDB; O74756; -.
DR PRO; PR:O74756; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0032153; C:cell division site; HDA:PomBase.
DR GO; GO:0071944; C:cell periphery; IBA:GO_Central.
DR GO; GO:0030428; C:cell septum; IBA:GO_Central.
DR GO; GO:0000935; C:division septum; IDA:PomBase.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0004100; F:chitin synthase activity; IGI:PomBase.
DR GO; GO:0006038; P:cell wall chitin biosynthetic process; IBA:GO_Central.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0140278; P:mitotic division septum assembly; IC:PomBase.
DR InterPro; IPR004835; Chitin_synth.
DR InterPro; IPR004834; Chitin_synth_fun.
DR InterPro; IPR013616; Chitin_synth_N.
DR PANTHER; PTHR22914; PTHR22914; 1.
DR Pfam; PF01644; Chitin_synth_1; 1.
DR Pfam; PF08407; Chitin_synth_1N; 1.
PE 3: Inferred from homology;
KW Cell cycle; Cell division; Cell wall biogenesis/degradation; Membrane;
KW Reference proteome; Septation; Transmembrane; Transmembrane helix.
FT CHAIN 1..926
FT /note="Chitin synthase-like protein 2"
FT /id="PRO_0000193717"
FT TRANSMEM 564..584
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 599..619
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 641..661
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 671..691
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 721..741
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 853..873
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 885..905
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..56
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 21..56
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 734..737
FT /note="CMDP -> VWIL (in Ref. 2; BAA87271)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 926 AA; 105579 MW; 7B880751AD53115E CRC64;
MSFQNPSYIN AKHRSFLQPK DTQDSQDLRN WVSHSSVDEE TAYSSSTLSS SSSKSFPCYD
EYDEIKSPDD QKVEYMKTLR TLEEDAFSYT DSVYDFEERS FDEHEPPIPP LHKTGVFSVP
LQPTHTVNSN SDDGYENSSK NEYLDFNSEI SASPVNEPMT HSQSYTSIDR LNSSSSHYSK
DVPLLCGSLT IDCPTPIDLR GMLGPFMQKN PDEASFLRYS AITCQPEDMN NNGLQLRTWS
TGRDIQIAVC LTLSDEDLAS FAISLSSIMN NLKHLCSRSK SRVWGNESWE KVLVCVVIDG
RNTVHQNVLD LLASIGVYQP HIAKGRVNGK RTLSHMYEFT STINVDEKLN LTTATGDGNV
PMQMLLCVKD RRLGTYNSHR WFLNGIASLA RPKVCLFVRN GARLGPTSIY HAWKAFDVDS
TIGGMCGKTS IDTGKFGFRL LNPFIASQHF DQMIHNNLRL PYDSCMGYIS NALNAIYGFR
YVALQDSYPN PGPLADYFEQ DQYEIPRRGI LQSNAFLAQE QLLFWKVITR KDAKWHLQYV
PEACATIEAP NSMAGILESK KSEINSSFSL AVYVIVDFFS LWTTRHKFFR FLLLTVQSLV
FAIEKLVNFF SMANFFLAFY FVCNATSYSS LNPYGNWARP LFLVFEYILI CLIFSQFMLA
MGNRPRSCRV LLFISTALFS IIMIYFVFCV FYISLIPLHN SDNSEIVLGN NYFTTLIFSS
LLILACYAFV SLICMDPFFI FTCIVQYILL MPTRIYTEQI YALCHLDDAS ISKDDNQQEF
NFDTGITHCS MNDEGYTTIS IPKANVLNSV YNSSLKNFSS SNDTSVYHDV QDHCYRKPQS
YEDHYQDIRT RYVLVWAVSN LILAIVLIQV FDGMRFINNG YMKYIFWSIV AFTAWKTMGA
VTFIATKIIS RIANCVKSKL YIFNDP
