ID   CHS2_SORBI              Reviewed;         401 AA.
AC   Q9SBL7;
DT   27-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=Chalcone synthase 2;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase 2;
GN   Name=CHS2;
OS   Sorghum bicolor (Sorghum) (Sorghum vulgare).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Andropogonodae; Andropogoneae; Sorghinae; Sorghum.
OX   NCBI_TaxID=4558;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Lo S.-C.C., Nicholson R.L.;
RT   "Molecular cloning of chalcone synthase genes from sorghum.";
RL   Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF152549; AAD41874.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9SBL7; -.
DR   SMR; Q9SBL7; -.
DR   STRING; 4558.Sb05g020170.1; -.
DR   eggNOG; ENOG502QRSY; Eukaryota.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..401
FT                   /note="Chalcone synthase 2"
FT                   /id="PRO_0000216056"
FT   ACT_SITE        168
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ   SEQUENCE   401 AA;  43718 MW;  6C61A33960F27CB5 CRC64;
     MAGATVTVEE VRKAQRATGP ATVLAIGTAT PANCVHQADY PDYYFRITKS EHMTELKEKF
     KRMCDKSQIR KRYMHLTEEY LAENPNMCAY MAPSLDARQD IVVVEVPKLG KAAAQKAIKE
     WGQPKSKITH LVFCTTSGVD MPGADYQLTK MLGLRPSVNR LMMYQQGCFA GGTVLRVAKD
     LAENNRGARV LVVCSEITAV TFRGPSESHL DSMVGQALFG DGAAAVIVGA DPDERVERPL
     FQLVSASQTI LPDSEGAIDG HLREVGLTFH LLKDVPGLIS KNIERSLEEA FKPLGITDYN
     SIFWVAHPGG PAILDQVEAK VGLEKERMRA TRHVLSEYGN MSSACVLFIL DEMRKRSAED
     GRATTGEGFE WGVLFGFGPG LTVETVVLHS VPITTGAAIT A