ID   CHS2_SOYBN              Reviewed;         388 AA.
AC   P17957;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1990, sequence version 1.
DT   25-MAY-2022, entry version 119.
DE   RecName: Full=Chalcone synthase 2;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase 2;
GN   Name=CHS2;
OS   Glycine max (Soybean) (Glycine hispida).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC   Glycine subgen. Soja.
OX   NCBI_TaxID=3847;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Williams;
RX   PubMed=2356130; DOI=10.1093/nar/18.11.3398;
RA   Akada S., Kung S.D., Dube S.K.;
RT   "Nucleotide sequence of one member of soybean chalcone synthase multi-gene
RT   family.";
RL   Nucleic Acids Res. 18:3398-3398(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Williams;
RX   PubMed=8108500; DOI=10.1104/pp.102.1.317;
RA   Akada S., Kung S.D., Dube S.K.;
RT   "Nucleotide sequence and putative regulatory elements of gene 2 of the
RT   soybean (Glycine max) chalcone synthase multigene family.";
RL   Plant Physiol. 102:317-319(1993).
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; X52097; CAA36317.1; -; Genomic_DNA.
DR   EMBL; X65636; CAA46590.1; -; Genomic_DNA.
DR   PIR; JQ2249; JQ2249.
DR   PIR; S16338; SYSYCN.
DR   RefSeq; XP_003524935.1; XM_003524887.3.
DR   AlphaFoldDB; P17957; -.
DR   SMR; P17957; -.
DR   PRIDE; P17957; -.
DR   InParanoid; P17957; -.
DR   UniPathway; UPA00154; -.
DR   Proteomes; UP000008827; Unplaced.
DR   GO; GO:0016747; F:acyltransferase activity, transferring groups other than amino-acyl groups; IBA:GO_Central.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0030639; P:polyketide biosynthetic process; IBA:GO_Central.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Flavonoid biosynthesis; Reference proteome; Transferase.
FT   CHAIN           1..388
FT                   /note="Chalcone synthase 2"
FT                   /id="PRO_0000216063"
FT   ACT_SITE        164
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
FT   CONFLICT        9
FT                   /note="K -> Q (in Ref. 2; CAA46590)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        100
FT                   /note="M -> V (in Ref. 2; CAA46590)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        299
FT                   /note="R -> W (in Ref. 2; CAA46590)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        387
FT                   /note="T -> S (in Ref. 2; CAA46590)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   388 AA;  42504 MW;  634D3F1CEDC5F973 CRC64;
     MVSVEEIRKA QRAEGPATVM AIGTATPPNC VDQSTYPDYY FRITNSEHMT ELKEKFKRMC
     DKSMIKKRYM YLNEEILKEN PSVCAYMAPS LDARQDMVVM EVPKLGKEAA TKAIKEWGQP
     KSKITHLIFC TTSGVDMPGA DYQLTKLLGL RPSVKRYMMY QQGCFAGGTV LRLAKDLAEN
     NKGARVLVVC SEITAVTFRG PTDTHLDSLV GQALFGDGAA AVIVGSDPLP VEKPLFQLVW
     TAQTILPDSE GAIDGHLREV GLTFHLLKDV PGLISKNIEK ALVEAFQPLG ISDYNSIFRI
     AHPGGPAILD QVEAKLGLKP EKMEATRHVL SEYGNMSSAC VLFILDQMRK KSIENGLGTT
     GEGLDWGVLF GFGPGLTVET VVLRSVTV