ID   CHS3_CAMSI              Reviewed;         389 AA.
AC   P48388;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=Chalcone synthase 3;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase 3;
GN   Name=CHS3;
OS   Camellia sinensis (Tea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; Ericales; Theaceae; Camellia.
OX   NCBI_TaxID=4442;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Yabukita; TISSUE=Leaf;
RX   PubMed=7820373;
RA   Takeuchi A., Matsumoto S., Hayatsu M.;
RT   "Chalcone synthase from Camellia sinensis: isolation of the cDNAs and the
RT   organ-specific and sugar-responsive expression of the genes.";
RL   Plant Cell Physiol. 35:1011-1018(1994).
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; D26595; BAA05642.1; -; mRNA.
DR   AlphaFoldDB; P48388; -.
DR   SMR; P48388; -.
DR   PRIDE; P48388; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..389
FT                   /note="Chalcone synthase 3"
FT                   /id="PRO_0000215962"
FT   ACT_SITE        164
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ   SEQUENCE   389 AA;  42815 MW;  827CB97E6246B188 CRC64;
     MVTVEDVWRA QRARGPATVL AIGTATPPNC VDQSTYPDYY FRITNSEHKV ELKEKFKRMC
     DKSMIKKRYM YLTEEILKEN PLVCEYMAPS LDARQDMVVV EVPKLGKEAA TKAIKEWGQP
     KSKITHLVFC TTSGVDMPGA DYQLTKLLGL RPSVKRLMMY QQGCFAGGTV LRLAKDLAEN
     NKGARVLVVC SEITAVTFRG PSDTHLDSLV GQSLFGDGAA AIIIGSDPIP EVEKPLFELV
     SAAQTILPSS DGAIDGHLRE VGLTFHLLKD VPRLISMNVE KSLVEAFQPL GISDWNSLFW
     IAHPGGPAIL DQVELKLGLK EEKLRATRHV LSEYGNMSSA CVLFILDEMR KKSAEEGLKT
     TGEGLEWGVL FGFGPGLTVE TVVLHSLCT