ID   CHS3_GERHY              Reviewed;         403 AA.
AC   P48392;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Chalcone synthase 3;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase 3;
GN   Name=CHS3;
OS   Gerbera hybrida (Daisy).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; campanulids; Asterales; Asteraceae; Mutisioideae; Mutisieae;
OC   Gerbera.
OX   NCBI_TaxID=18101;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Corolla;
RX   PubMed=7787187; DOI=10.1007/bf00042037;
RA   Helariutta Y., Elomaa P., Kotilainen M., Griesbach R.J., Schroeder J.,
RA   Teeri T.H.;
RT   "Chalcone synthase-like genes active during corolla development are
RT   differentially expressed and encode enzymes with different catalytic
RT   properties in Gerbera hybrida (Asteraceae).";
RL   Plant Mol. Biol. 28:47-60(1995).
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; Z38098; CAA86220.1; -; mRNA.
DR   PIR; S56701; S55464.
DR   AlphaFoldDB; P48392; -.
DR   SMR; P48392; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..403
FT                   /note="Chalcone synthase 3"
FT                   /id="PRO_0000215978"
FT   ACT_SITE        170
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ   SEQUENCE   403 AA;  44129 MW;  819E10C6E4255140 CRC64;
     MATSPAVIDV ETIRKAQRAE GPATILAIGT ATPANCVYQA DYPDYYFRVT ESEHMVDLKE
     KFQRMCDKSM IRKRYMHITE EFLKENPSMC KFMAPSLDAR QDLVVVEVPK LGKEAATKAI
     KEWGFPKSKI THLVFCTTSG VDMPGADYQL TKLLGLRPSV KRLMMYQQGC FAGGTVLRLA
     KDLAENNKGA RVLVVCSEIT AVTFRGPNEG HLDSLVGQAL FGDGAAAVII GSDPDLSVER
     PLFEMVSAAQ TILPDSEGAI DGHLKEVGLT FHLLKDVPAL IAKNIEKALI QAFSPLNIND
     WNSIFWIAHP GGPAILDQVE FKLGLREEKL RASRHVLSEY GNMSSACVLF ILDEMRKKSI
     KDGKTTTGEG LEWGVLFGFG PGLTVETVVL HSLPATISVA TQN