ID   CHS3_PEA                Reviewed;         389 AA.
AC   O23883;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 87.
DE   RecName: Full=Chalcone synthase 3;
DE            EC=2.3.1.74;
DE   AltName: Full=Naregenin-chalcone synthase 3;
GN   Name=CHS3;
OS   Pisum sativum (Garden pea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Fabeae; Pisum.
OX   NCBI_TaxID=3888;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Midoriusui;
RX   PubMed=9230896; DOI=10.1007/s004380050471;
RA   Ito M., Ichinose Y., Kato H., Shiraishi T., Yamada T.;
RT   "Molecular evolution and functional relevance of the chalcone synthase
RT   genes of pea.";
RL   Mol. Gen. Genet. 255:28-37(1997).
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; D88261; BAA22043.1; -; Genomic_DNA.
DR   AlphaFoldDB; O23883; -.
DR   SMR; O23883; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..389
FT                   /note="Chalcone synthase 3"
FT                   /id="PRO_0000216020"
FT   ACT_SITE        164
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ   SEQUENCE   389 AA;  42802 MW;  70720C8BCC15CAB8 CRC64;
     MVSVSEIRKA QRAEGPATIL AIGTANPANC VEQSTYPDFY FRITNSEHKT ELKQKFQRMC
     DKSMINRRYM YLTEEILKEN PSVCEYMAPS LDARQDMVVV EVPRLGKEAA VKAIKEWGQP
     KSKITHLIFC TTSGVDMPGA DYQLTKLLGL RPYVKRYMMY QQGCFAGGTV LRLAKDLAEN
     NKGARVLVVC SEVTAVTFRG PSDTHLDSLV GQALFGDGAA ALIVGSDPLP EIENPIFEMV
     WTAQTIAPDS EGAIDGHLRE AGLTFHLLKD VPAIVSKNID KALVEAFQPL GISDYNSIFW
     IAHPGGPAIL DQVEQKLALK PEKMKATREV LSEYGNMSSA CVLFILDEMR RKSIQNGLKT
     TGEGLEWGVL FGFGPGLTIE TVVLHSVAI