ID   CHS3_RUTGR              Reviewed;         393 AA.
AC   Q9FSB7;
DT   25-MAR-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Chalcone synthase 3;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase 3;
GN   Name=CHS3;
OS   Ruta graveolens (Common rue).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Sapindales; Rutaceae; Rutoideae; Ruta.
OX   NCBI_TaxID=37565;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Immature flower;
RX   PubMed=11054106; DOI=10.1046/j.1432-1327.2000.01746.x;
RA   Springob K., Lukacin R., Ernwein C., Groening I., Matern U.;
RT   "Specificities of functionally expressed chalcone and acridone synthases
RT   from Ruta graveolens.";
RL   Eur. J. Biochem. 267:6552-6559(2000).
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; AJ297791; CAC14061.2; -; mRNA.
DR   AlphaFoldDB; Q9FSB7; -.
DR   SMR; Q9FSB7; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..393
FT                   /note="Chalcone synthase 3"
FT                   /id="PRO_0000216047"
FT   ACT_SITE        166
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ   SEQUENCE   393 AA;  43120 MW;  3FAAA0E499BCD14A CRC64;
     MAAVTVEEIR KAQRADGPAT VLAIGTATPA NYVTQADYPD YYFRITKSEH MTDLKEKFKR
     MCDKSMIRKR YMHLTEEILK ENPNMCAYMA PSLDARQDIV VVEVPKLGKE AAAKAIKEWG
     QPKSKITHLI FCTTSGVDMP GCDYQLTKLL GLRPSVKRFM MYQQGCFAGG TVLRLAKDLA
     ENNRGARVLV VCSEITAVTF RGPVDTHLDS LVGQALFGDG AAAVIVGADP DESIERPLYQ
     LVSAAQTILP DSDGAIDGHL REVGLTFHLL KDVPGLISKN IEKSLKEAFG PIGISDWNSI
     FWIAHPGGPA ILDQVEEKLE LKEEKLRATR HVLSEYGNMS SACVLFILDE MRKKCAEEGM
     ATTGEGLEWG VLFGFGPGLT VETVVLRSVP IKA